Preparation and characterization of aspirin-fucoidan complex and its admirable antitumor activity on human non-small cell lung cancer cells.

The purpose of this work is to prepare a novel acetylated derivative of Undaria pinnatifida fucoidan (UPFUC) with admirable antitumor activity. Fucoidan was first acetylated by acetylsalicylic acid (aspirin, ASA) to form the ASA-UPFUC complex. The antitumor efficacy results stated that ASA-UPFUC inhibited the proliferation of human non-small cell lung cancer A549 cells in a dose-dependent manner, with an IC value of 49.

Purification and Identification of Peptides from with Cytoprotective and Antioxidative Effect against HO-Treated HepG2 Cells.

Antioxidant peptides were purified from () protein hydrolysate by ultrafiltration, gel filtration chromatography, and semipreparative reversed-phase HPLC and identified by UPLC-ESI-MS/MS. Therein, TCLGPK and TCLGER were selected to be synthesized, and they displayed desirable radical-scavenging activity to ABTS (99.20 ± 0.

Oroxylin a promoted apoptotic extracellular vesicles transfer of glycolytic kinases to remodel immune microenvironment in hepatocellular carcinoma model.

Although oroxylin A, a natural flavonoid compound, suppressed progression of hepatocellular carcinoma, whether the tumor microenvironment especially the communication between cancer cells and immune cells was under its modulation remained obscure. Here we investigated the effect of extracellular vesicles from cancer cells elicited by oroxylin A on macrophages in vitro. The data shows oroxylin A elicits apoptosis-related extracellular vesicles through caspase-3-mediated activation of ROCK1in HCC cells, which regulates M1-like polarization of macrophage.

Single-cell RNA sequencing reveals the heterogeneity and microenvironment in one adenoid cystic carcinoma sample.

Adenoid cystic carcinoma (ACC) is one of the most common malignancy of the major salivary glands with a high recurrence rate and poor prognosis. Determining tumor heterogeneity and factors in the microenvironment may provide novel therapeutic targets for ACC. We performed single-cell RNA sequencing of one ACC sample and normal salivary gland tissues from a patient to analyze tumor heterogeneity, immunosuppressive landscape, and intercellular communication networks.

Transcriptomic insights into adenoid cystic carcinoma via RNA sequencing.

The aim of this study was to investigate the underlying mechanisms of adenoid cystic carcinoma (ACC) at the transcriptome level. We obtained paired tumor and normal salivary gland tissues from 15 ACC patients, which were prepared for RNA sequencing. Gene enrichment analysis revealed that the upregulated pathways were mainly involved in axonogenesis, and the downregulated pathways were mainly related to leukocyte migration, the adaptive immune response, lymphocyte-mediated immunity, and the humoral immune response.

Production and characterization of exopolysaccharides from salinity-induced Auxenochlorella protothecoides and the analysis of anti-inflammatory activity.

The set scenario of this work was to investigate the production, physicochemical characteristics, and anti-inflammatory activities of exopolysaccharides from salinity-induced Auxenochlorella protothecoides. The results demonstrated that 10 ‰ salinity manipulation endowed preferable exopolysaccharide production by A. protothecoides.

Human umbilical cord mesenchymal stromal cell-derived exosomes protect against MCD-induced NASH in a mouse model.

Background And Aims: Human umbilical cord mesenchymal stem cells (hUC-MSCs) are increasingly being studied in clinical trials of end-stage liver disease because of their good tissue repair and anti-inflammatory effects. hUC-MSC exosomes are vesicles with spherical structures secreted by cells that produce them. The diameter of exosomes is much smaller than that of hUC-MSCs, suggesting that exosomes might be a novel and safer therapeutic product of mesenchymal stem cells.

Rhizopus oryzae fermentation wastewater nutrient removal coupling protein fodder production by employing Chlorella pyrenoidosa.

The scenario was to investigate feasibilities of employing Chlorella pyrenoidosa for Rhizopus oryzae fermentation wastewater nutrient removal coupling protein fodder production. Results stated that TN, TP, NH-N, COD, BOD removal reached 99.79%, 94.

A preliminary study on preparation, characterization, and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava.

Polysaccharides derived from edible mushrooms were sources of new prebiotic compounds. Limited studies of their prebiotic effects, as well as the presence of residual dark colors, impede their use as prebiotics in the food industry. To boost the prebiotic value of polysaccharides from the edible mushroom Ramaria flava, a decolorization method, and the physicochemical characterization and prebiotic potential of the decolorized polysaccharide (DRFP) were investigated in this study.

Reconstruction and analysis of the aberrant lncRNA-miRNA-mRNA network based on competitive endogenous RNA in adenoid cystic carcinoma of the salivary gland.

Background: The aim of this work was to investigate the competing endogenous RNA (ceRNA) network in adenoid cystic carcinoma of the salivary gland (SACC).

Methods: Differentially expressed lncRNAs (DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs) between cancer tissues and normal salivary gland (NSG) in ACC were identified using data from the Gene Expression Omnibus (GEO) database. Functional annotation and pathway enrichment analysis of DEmRNAs were performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.

Limited Clinical Utility of Lipid-Laden Macrophage Index of Induced Sputum in Predicting Gastroesophageal Reflux-Related Cough.

Gastroesophageal reflux disease (GERD) is a common cause of chronic cough (CC). However, the diagnosis of GERD associated with CC based on 24-hour esophageal pH-monitoring or favorable response to empirical anti-reflux trials is invasive and time-consuming. Lipid-laden macrophages (LLMs) are supposed to be a biomarker for micro-aspiration of gastric content in the respiratory tract.

Ginsenoside Rb3 Alleviates the Toxic Effect of Cisplatin on the Kidney during Its Treatment to Oral Cancer via TGF--Mediated Mitochondrial Apoptosis.

Objective: The research aimed to confirm the role of the transforming growth factor- (TGF-) in cisplatin- (CPT-) evoked kidney toxicity and elucidate the mechanism that ginsenoside Rb3 (Rb3) could alleviate the kidney toxicity by CPT during its treatment to oral cancer via TGF--mediated mitochondrial apoptosis.

Methods: The model of xenograft nude mice bearing oral carcinoma cells ACC83 was established and treated with CPT and/or Rb3, respectively. Bodyweights of the treated mice were weighed, and the kidney tissues were collected; following, the histopathology and the expression of TGF- were examined using H&E staining and immunohistochemistry.

Super and selective adsorption of cationic dyes using carboxylate-modified lignosulfonate by environmentally friendly solvent-free esterification.

Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and XPS were used to confirm successful preparation of M-LSP. The result is that: M-LSP is apt to adsorb cationic dye.

The growth and lutein accumulation in heterotrophic Chlorella protothecoides provoked by waste Monascus fermentation broth feeding.

Although the potential of heterotrophic microalgae served as a sustainable source for lutein, it was still crucial to formulate a suitable medium to offset the cost involved in algal biomass cultivation while improve inherent lutein productivity. The objective of this study was to investigate the feasibilities of waste Monascus fermentation broth medium (MFBM) toward heterotrophic Chlorella protothecoides-enriched lutein. The results indicated that C.

The potential growth and lipid accumulation in Coccomyxa subellipsoidea triggered by glucose combining with sodium acetate.

Carbon sources whether types or magnitudes were fateful in terms of stimulating growth and lipids accumulation in microalgae applied for biodiesel production. The set scenario of this work was to investigate the feasibilities of glucose (G) combining with sodium acetate (SA) carbon sources in enhancing biomass and lipid accumulation in Coccomyxa subellipsoidea. The results demonstrated that C.

Robust block sparse discriminative classification framework.

In this paper, a block sparse discriminative classification framework (BSDC) is proposed under the assumption that a block or group structure exists in sparse coefficients on classification. First, we propose a block discriminative dictionary-learning (BDDL) algorithm, which learns class-specific subdictionaries and forces the sparse coefficients to be block sparse. An efficient gradient-based optimization strategy of BDDL also is developed, and the block sparse constraint of the sparse coefficient leads to a least-squares solution of nonzero entries in the sparse coding stage of dictionary learning.

Determination of trace amount of formaldehyde base on a bromate-Malachite Green system.

A novel catalytic kinetic spectrophotometric method for determination of trace amount of formaldehyde (FA) has been established, based on catalytic effect of trace amount of FA on the oxidation of Malachite Green (MG) by potassium bromate in presence of sulfuric acid medium, and was reported for the first time. The method was monitored by measuring the decrease in absorbance of MG at 617 nm and allowed a precise determination of FA in the range of 0.003-0.

The catalytic kinetic method for the determination of trace formaldehyde (FA) base on a bromate-eosin Y system.

A new simple and highly sensitive catalytic kinetic method for the determination of trace amount of FA in food sample has been established. The method was based on the catalytic effect of FA on the oxidation of eosin Y by potassium bromate in present of phosphoric acid. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of eosin Y at 518 nm.

EDTA and electricity synergetic catalyzed Fe(3+)/H2O2 process for amoxicillin oxidation.

Three oxidation processes for amoxicillin wastewater pretreatment such as Electro-Fe(3+)(EDTA)/H2O2 (EDTA: ethylenediaminetetraacetic acid), Fe(3+)(EDTA)/H2O2 and Electro-Fe(3+)/H2O2 were simultaneously discussed at pH of 7.0 (+/-0.1).

Isoform-specific inhibition of TRPC4 channel by phosphatidylinositol 4,5-bisphosphate.

Full-length transient receptor potential (TRP) cation channel TRPC4alpha and shorter TRPC4beta lacking 84 amino acids in the cytosolic C terminus are expressed in smooth muscle and endothelial cells where they regulate membrane potential and Ca(2+) influx. In common with other "classical" TRPCs, TRPC4 is activated by G(q)/phospholipase C-coupled receptors, but the underlying mechanism remains elusive. Little is also known about any isoform-specific channel regulation.

p53 down-regulates phosphatase and tensin homologue deleted on chromosome 10 protein stability partially through caspase-mediated degradation in cells with proteasome dysfunction.

There has been intense investigation regarding the interaction between the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) and p53 tumor suppressors. p53 has been shown to up-regulate PTEN expression as a transcriptional activator. However, clinical observations by immunohistochemistry studies indicate that significant increases in p53 protein levels coexist with reduced or absent expression of PTEN protein in a variety of neoplasias.

PTEN autoregulates its expression by stabilization of p53 in a phosphatase-independent manner.

PTEN (phosphatase and tensin homologue, deleted on chromosome 10) is a tumor suppressor with dual phosphatase activity and mutations of its gene, PTEN, have been associated with many sporadic cancers and heritable neoplasia syndromes, including Cowden syndrome and Bannayan-Riley-Ruvalcaba syndrome. However, accumulating evidence now shows that PTEN may have novel functions other than as a phosphatase. In the present study, we show that PTEN is able to autoregulate its expression through the stabilization of another tumor suppressor p53.