Publications by authors named "Yufan Xian"
Considerable effort has been invested in developing salicylic acid (SA) biosensors for various application purposes. Here, by engineering the sensing modules and host cell chassis, we have gradually optimized the NahR-Psal/Pr-based SA biosensor, increasing the sensitivity and maximum output by 17.2-fold and 9.
View Article and Find Full Text PDFPolysaccharide hydrolases are enzymes capable of hydrolyzing polysaccharides to generate oligosaccharides that have diverse applications in the food, feed and pharmaceutical industries. However, the detailed mechanisms governing the compositions of their hydrolysates remain poorly understood. Previously, we identified a novel neopullulase Amy117, which exclusively converts pullulan to panose by specifically cleaving α-1,4-glycosidic bonds.
View Article and Find Full Text PDFArticle Synopsis
- SUMO modification is an important regulatory process in eukaryotes that involves a specific protease called Ulp1, which cleaves the SUMO protein at the C-terminal Gly-Gly motif.
- Research revealed that Ulp1 can still cleave substrates even when the Gly-Gly motif is mutated, suggesting this motif isn't strictly necessary for the cleavage process.
- The study's structural analysis showed that Ulp1's unique active site allows it to specialize in cleaving small residues, and the P1' position, particularly an Ala residue, plays a crucial role in ensuring accurate cleavage, highlighting possible areas for drug discovery related to SUMOylation-related diseases.