Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay.

Digital nucleic acid assays, known for their high sensitivity and specificity, typically rely on fluorescent readouts and expensive and complex nanowell manufacturing, which constrain their broader use in point-of-care (POC) application. Here, we introduce an alternative digital molecular diagnostics, termed dCRISTOR, by seamlessly integrating deactivated Cas9 (dCas9)-engineered micromotors, extraction-free loop-mediated isothermal amplification (LAMP), low-cost bright field microscopy, and deep learning-enabled image processing. The micromotor, composed of a polystyrene sphere attached to a magnetic bead, incorporates a dCas9 ribonucleoprotein complex.

A Diffusion Model Based on Network Intrusion Detection Method for Industrial Cyber-Physical Systems.

Industrial Cyber-Physical Systems (ICPS) connect intelligent manufacturing equipment equipped with sensors, wireless and RFID communication technologies through data interaction, which makes the interior of the factory, even between factories, become a whole. However, intelligent factories will suffer information leakage and equipment damage when being attacked by ICPS intrusion. Therefore, the network security of ICPS cannot be ignored, and researchers have conducted in-depth research on network intrusion detection for ICPS.