Enhancing academic engagement through students' perceptions of teacher expectations: the mediating role of intentional self-regulation in middle school.

In the context of evolving educational standards, enhancing students' academic engagement has emerged as a critical factor in mitigating the risks of school aversion among middle school students. This study examines the longitudinal effect of middle school students' perceptions of teacher expectations on their academic engagement, as well as the mediating role of intentional self-regulation in this dynamic. A six-month longitudinal survey was conducted with 702 Chinese middle school students through three waves of questionnaires.

Designer cellular spheroids with DNA origami for drug screening.

Current in vitro models struggle to balance the complexity of human diseases with suitability for large-scale drug tests. While 3D cultures simulate human tissues, they lack cellular intricacy, and integrating these models with high-throughput drug screening remains a challenge. Here, we introduce a method that uses self-assembling nucleic acid nanostructures decorated living cells, termed NACs, to create spheroids with a customizable 3D layout.

Engineered geranyl diphosphate methyltransferase produces 2-methyl-dimethylallyl diphosphate as a noncanonical C unit for terpenoid biosynthesis.

Terpenoids constitute the largest class of natural products with complex structures, essential functions, and versatile applications. Creation of new building blocks beyond the conventional five-carbon (C) units, dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate, expands significantly the chemical space of terpenoids. Structure-guided engineering of an -adenosylmethionine-dependent geranyl diphosphate (GPP) C2-methyltransferase from yielded variants converting DMAPP to a new C unit, 2-methyl-DMAPP.

DNA origami-based artificial antigen-presenting cells for adoptive T cell therapy.

Nanosized artificial antigen-presenting cells (aAPCs) with efficient signal presentation hold great promise for in vivo adoptive cell therapy. Here, we used DNA origami nanostructures as two-dimensional scaffolds to regulate the spatial presentation of activating ligands at nanoscale to construct high-effective aAPCs. The DNA origami-based aAPC comprises costimulatory ligands anti-CD28 antibody anchored at three vertices and T cell receptor (TCR) ligands peptide-major histocompatibility complex (pMHC) anchored at three edges with varying density.

Experimental and Numerical Study of a Rebar-Prestressed Cylinder Concrete Pipe (RPCCP) under Internal Load.

In order to study the load-bearing failure characteristics of a RPCCP under internal load, a field prototype test was designed, and a finite element model was established. An internal load was applied up to 2.0 MPa step by step and the force variation law of each part was obtained.

The impact of job-related stress on township teachers' professional well-being: A moderated mediation analysis.

This study aimed to explore the relationship between job-related stress and township teachers' professional well-being. Based on Job Demand-Resource Model, this study examined the mediating role of teachers' professional identity and the moderating role of perceived organizational support in this relationship. A total of 24,276 township teachers in China responded to the Teacher Stress Scale, the Teachers' Professional Identity Scale, the Teachers' Professional Well-Being Structure Questionnaire, and the Perceived Organizational Support Scale.

Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8 T cell detection.

Peptide-MHC (pMHC) multimers have excelled in the detection of antigen-specific T cells and have allowed phenotypic analysis using other reagents, but their use for detection of low-affinity T cells remains a challenge. Here we develop a multimeric T cell identifying reagent platform using two-dimensional DNA origami scaffolds to spatially organize pMHCs (termed as dorimers) with nanoscale control. We show that these dorimers enhance the binding avidity for low-affinity antigen-specific T cell receptors (TCRs).

Protective effects of apple polyphenols on bone loss in mice with high fat diet-induced obesity.

Obesity-induced inflammation can lead to an imbalance in bone formation and resorption. Our previous studies have demonstrated that apple polyphenols (APs) can reduce body weight and inflammation. But their effect on bone is still unclear.

The Genome-Wide Identification of Long Non-Coding RNAs Involved in Floral Thermogenesis in Gaertn.

The sacred lotus ( Gaertn.) can maintain a stable floral chamber temperature when blooming, despite ambient temperature fluctuations; however, the long non-coding RNAs (lncRNAs) involved in floral thermogenesis remain unclear. In the present study, we obtain comprehensive lncRNAs expression profiles from receptacles at five developmental stages by strand-specific RNA sequencing to reveal the lncRNAs regulatory mechanism of the floral thermogenesis of .

Nucleic Acid-Based Cell Surface Engineering Strategies and Their Applications.

The cell membrane is a biological interface regulating the communications between cells and their environment. The ability to functionalize the cell membrane with molecules or nanomaterials allows us to manipulate cellular behaviors and to expand cellular functions. Due to their unique merits of synthetic accessibility, flexible design, and precise programmability, nucleic acids provide an emerging and promising molecular toolkit for cell surface engineering.

DIA-Based Quantitative Proteomics Reveals the Protein Regulatory Networks of Floral Thermogenesis in .

The sacred lotus () can maintain a stable floral chamber temperature between 30 and 35 °C when blooming despite fluctuations in ambient temperatures between about 8 and 45 °C, but the regulatory mechanism of floral thermogenesis remains unclear. Here, we obtained comprehensive protein profiles from receptacle tissue at five developmental stages using data-independent acquisition (DIA)-based quantitative proteomics technology to reveal the molecular basis of floral thermogenesis of . A total of 6913 proteins were identified and quantified, of which 3513 differentially abundant proteins (DAPs) were screened.

DNA-Scaffolded Disulfide Redox Network for Programming Drug-Delivery Kinetics.

In response to specific stimuli, dynamic covalent materials enable the generation of new structures by reversibly forming/breaking chemical bonds, thus showing great potential for application in controlled drug release. However, using dynamic covalent chemistry to program drug-delivery kinetics remains challenging. Herein, an in situ polymerization-generated DNA-scaffolded disulfide redox network (DdiSRN) is reported in which nucleic acids are used as a scaffold for dynamic disulfide bonds.

Fe, N-doped carbon spheres prepared by electrospinning method as high efficiency oxygen reduction catalyst.

Electrocatalysts for the oxygen reduction reaction (ORR) are crucial in metal-air batteries, fuel cells and other electrochemical devices. In this study, iron and nitrogen co-doped carbon sphere electrocatalysts were synthesized by electrospinning and thermal treatment. According to the results, the catalyst marked as Fe-N/MCS-181 (Fe, N-doped mesoporous carbon spheres, iron nitrate nonahydrate as the iron source) has not only the highest iron content, which reaches up to 0.

The Effect of Inner Friction on Concrete Fracture Behavior under Biaxial Compression: A 3D Mesostructure Study.

The mechanical behavior of concrete under biaxial loading condition (especially biaxial compression) is one of the most important indexes to evaluate the quality of concrete. To study the mechanical behavior of concrete under biaxial compression at mesoscale, we adopted our recently developed 3D numerical model based on Voronoi tessellation and cohesive elements. A constitutive model considering the friction effect is used in the model to characterize the fracture behavior of all potential fracture surfaces inside the concrete.

The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of Ub expression.

The thermosensitive genic male sterile 5 (tms5) mutation causes thermosensitive genic male sterility in rice (Oryza sativa) through loss of RNase Z function, which influences ubiquitin fusion ribosomal protein L40 (Ub ) messenger RNA levels during male development. Here, we used ATAC-seq, combined with analysis of H3K9ac and H3K4me2, to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wuxiang S (WXS) derived from a mutant tms5 allele. Furthermore, RNA-seq and bioinformatic analyses identified specific transcription factors (TFs) in differentially accessible chromatin regions.

Circular RNA profiling of the rice photo-thermosensitive genic male sterile line Wuxiang S reveals circRNA involved in the fertility transition.

Background: Circular RNAs (circRNAs) are known to play an important role in the regulation of gene expression in eukaryotes. Photo-thermosensitive genic male sterile (PTGMS) is a very important germplasm resource in two-line hybrid rice breeding. Although many circRNAs have been identified in rice (Oryza sativa L.

[Study on interaction between ginsenosides Rg_1,Rb_1 and Ro and bovine serum albumin].

Small molecules with physiological or pharmacological activities need to interact with biological macromolecules in order to function in the body. As the protein with the highest proportion of plasma protein,serum albumin is the main protein binding to various endogenous or exogenous small molecules. Serum albumin interacts with small molecules in a reversible non-covalent manner and transports small molecules to target sites.

Fracture Behavior and Energy Analysis of 3D Concrete Mesostructure under Uniaxial Compression.

In order to investigate the fracture behavior of concrete mesostructure and reveal the inner failure mechanisms which are hard to obtain from experiments, we develop a 3D numerical model based on the Voronoi tessellation and cohesive elements. Specifically, the Voronoi tessellation is used to generate the aggregates, and the cohesive elements are applied to the interface transition zone (ITZ) and the potential fracture surfaces in the cement matrix. Meanwhile, the mechanical behavior of the fracture surfaces is described by a modified constitutive which considers the slips and friction between fracture surfaces.

[Prevalence of spp. infections in large - scale pig farms in north of Yangtze River in Anhui Province].

Objective: To characterize the prevalence and species/genotypes of spp. in farmed pigs in the north of the Yangtze River in Anhui Province.

Methods: A total of 500 samples of pig feces were obtained from seven largescale pig farms in the north of the Yangtze River in Anhui Province.

Extracting Cross-Sectional Clinical Images Based on Their Principal Axes of Inertia.

Cross-sectional imaging is considered the gold standard in diagnosing a range of diseases. However, despite its widespread use in clinical practice and research, no widely accepted method is available to reliably match cross-sectional planes in several consecutive scans. This deficiency can impede comparison between cross-sectional images and ultimately lead to misdiagnosis.