A dual-emission fluorescent biosensing method was developed for simultaneous determination of CaMV35S and NOS in genetically modified (GM) plants. Two designed hairpin DNA (H1, H2) sequences were used as templates to synthesize H1-AgNCs (λ = 570 nm, λ = 625 nm) and H2-AgNCs (λ = 470 nm, λ = 555 nm). By using H1-AgNCs and H2-AgNCs as dual-signal tags, combined with signal amplification strategy of magnetic separation to reduce background signal and an enzyme-free catalytic hairpin assembly (CHA) signal amplification strategy, a novel multi-target fluorescent biosensor was fabricated to detect multiple targets based on FRET between signal tags (donors) and magnetic FeO modified graphene oxide (FeO@GO, acceptors).
View Article and Find Full Text PDFSimultaneous detection of multiple foodborne pathogens is of great importance for ensuring food safety. Herein, we present a sensitive dual-channel electrochemical biosensor based on copper metal organic frameworks (CuMOF) and lead metal organic framework (PbMOF) for simultaneous detection of Salmonella typhimurium (S. typhimurium) and Listeria monocytogenes (L.
View Article and Find Full Text PDFObjective: Microscopy was used to characterize platelet--infected erythrocyte interactions in patients infected with , , or , and to investigate the relationship between platelet-associated parasite killing and parasite clearance.
Methods: Data from 244 malaria patients admitted to the Fourth People's Hospital of Nanning between 1 January 2011 and 30 September 2022, and 45 healthy controls, were collected prospectively and assessed retrospectively. Characteristics of platelet-erythrocyte interactions were visualized by microscopy, and blood cell count and clinical profiles of these participants were obtained from the electronic medical records.
Front Cell Infect Microbiol
November 2022
Objectives: This study aimed to investigate the clinical and biochemical profiles of patients with imported malaria infection between 1 January 2011 and 30 April 2022 and admitted to the Fourth People's Hospital of Nanning.
Methods: This cohort study enrolled 170 patients with conformed imported malaria infection. The clinical and biochemical profiles of these participants were analyzed with malaria parasite clearance, and signs and symptoms related to malaria disappearance were defined as the primary outcome.
In this work, we demonstrated an in situ approach for doping CsPbBr nanocrystals (NCs) with In and Cl with a ligand-assisted precipitation method at room temperature. The In and Cl co-doped NCs are characterized by the powder x-ray diffraction patterns, ultraviolet-visible, photoluminescence (PL) spectroscopy, time-resolved PL (TRPL), ultraviolet photoelectron spectroscopy, x-ray photoelectron spectroscopy, and transmission electron microscopy. Based on PL and TRPL results, the non-radiative nature of In-doping induced localized impurity states is revealed.
View Article and Find Full Text PDFMitochondrial DNA B Resour
January 2020
(Bleeker), is a small-sized benthopelagic fish with ornamental value. In the present study, the complete mitochondrial genome of was sequenced and determined. The complete mitogenome of was 16,683 bp in length, consisting of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 2 non-coding regions.
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