[ degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit].

Objective: To investigate the degradation and histocompatibility of modified chitosan based on conductive composite nerve conduit, so as to provide a new scaffold material for the construction of tissue engineered nerve.

Methods: The nano polypyrrole (PPy) was synthesized by microemulsion polymerization, blended with chitosan, and then formed conduit by injecting the mixed solution into a customized conduit formation model. After freeze-drying and deacidification, the nano PPy/chitosan composite conduit (CP conduit) was prepared.

[Preparation and biocompatibility of nano polypyrrole/chitin composite membrane].

Objective: To prepare nano polypyrrole (PPy)/chitin composite membrane and observe their biocompatibility.

Methods: The nano PPy was synthesized by microemulsion polymerization, blended with chitosan and then formed membranes. The membranes were then modified by acetylation to get the experimental membranes (nano PPy/chitin composite membranes, group A).

[Effect of short-term low-frequency electrical stimulation on nerve regeneration of delayed nerve defect during operation].

Objective: To explore the effect of short-term low-frequency electrical stimulation (SLES) during operation on nerve regeneration in delayed peripheral nerve injury with long gap.

Methods: Thirty female adult Sprague Dawley rats, weighing 160-180 g, were used to prepare 13-mm defect model by trimming the nerve stumps. Then all rats were randomly divided into 2 groups, 15 rats in each group.

Morphological Proof of nerve regeneration after long-term defects of rat sciatic nerves.

Unsatisfactory efficacy of clinical cure for long-term delayed injuries and other disadvantages such as the low regeneration rate and speed of axotomized neurons and the questionable reinnervation ability of atrophic target organ lead to inaction to the long-term delayed injuries. Here we attempted to use autologous nerve to bridge a long-term delayed 10-mm defect in SD rats based on some previous positive messages of basic and clinical research. In this study, for experimental groups, the rat sciatic nerve had been transected leaving a 10-mm defect, which was maintained for 3 or 6 months before implantation with the autologous graft.

Cerebellar interpositus nuclear and gastric vagal afferent inputs reach and converge onto glycemia-sensitive neurons of the ventromedial hypothalamic nucleus in rats.

The glycemia-sensitive neurons of the ventromedial hypothalamic nucleus (VMN) have traditionally been implicated in feeding regulation. Some studies reported that the neuronal activity of the VMN could be modulated by inputs from the gastric vagal afferent, and the cerebellum might participate in regulating non-somatic visceral activities via the cerebellohypothalamic projections. The present study was therefore undertaken to investigate whether the inputs from the gastric vagal nerves and the cerebellar interpositus nucleus (IN) could reach and converge onto single VMN neurons, especially those glycemia-sensitive ones.