Preparation and immunogenicity evaluation of C-HapS-P6 fusion protein vaccine against nontypeable Haemophilus influenzae in mice.

Nontypeable Haemophilus influenzae (NTHi) is the dominant pathogen in several infectious diseases. Currently the use of antibiotics is the main intervention to prevent NTHi infections, however with the emergence of drug resistant strains, it has compromised the treatment of respiratory infections with antibiotics. Therefore there is an urgent need to develop a safe and effective vaccine to prevent NTHi infections.

Mucosal immunity of mannose-modified chitosan microspheres loaded with the nontyepable Haemophilus influenzae outer membrane protein P6 in BALB/c mice.

Nontypeable Haemophilus influenzae (NTHi) is a common opportunistic pathogen that colonizes the nasopharynx. NTHi infections result in enormous global morbidity in two clinical settings: otitis media in children and acute exacerbation of chronic obstructive pulmonary disease (COPD) in adults. Thus, there is an urgent need to design and develop effective vaccines to prevent morbidity and reduce antibiotic use.

sp. nov., isolated from rhizosphere soil of .

A psychrotolerant actinobacterium, designated strain J5903, was isolated from an alkaline soil sample from the rhizosphere of collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China. Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci. Strain J5903 grew optimally at 20‒25 °C, at pH 7.

[Mouse macrophage-derived chemokine as an adjuvant enhances the protective effect of P6 protein vaccine of nontypeable Haemophilus influenzae].

Objective: To express P6 protein of nontypeable Haemophilus influenzae (NTHi) in prokaryotic cells and observe the immune effect of macrophage-derived chemokine (MDC) as an adjuvant on NTHi-P6 protein vaccine.

Methods: The encoding sequence of NTHi-P6 protein was inserted into plasmid PGEX-6P2, and the recombinant plasmid PGEX-6P2/P6 was transformed into E.coli XL1-Blue.

Genome sequence of Streptomyces sp. strain TOR3209, a rhizosphere microecology regulator isolated from tomato rhizosphere.

Streptomyces sp. strain TOR3209, isolated from tomato rhizosphere, can regulate the rhizosphere microecology of a variety of crops. Strain TOR3209 could improve plant systemic resistance and promote plant growth.