Evaluation of microgaps and microleakage at the implant-abutment interface of individualized abutments in the maxillary anterior region under functional loading: A pragmatic clinical trial.

Purpose: This study aimed to evaluate the effect of functional loading on microgaps and microleakage at implant-abutment interfaces (IAIs) in the aesthetic zone when using different abutments and to provide a clinical reference for abutment selection.

Methods: This study included 30 patients with 36 implants divided into three groups: zirconia (Zr)-one-piece custom abutment, titanium (Ti)-custom abutment, and Ti-original abutment. Scanning electron microscopy was used to examine alterations in the microgaps at the IAIs under functional loading.

Down-regulation of E2F1 attenuates UVB-induced human lens epithelial cell oxidative stress and pyroptosis through inhibiting NLRP3.

Background: It is well-known that ultraviolet B (UVB) causes cataracts by inducing pyroptosis and the production of reactive oxygen species (ROS) in human lens epithelial cells (HLECs). The transcription factor E2F1 (E2F1) serves as a positive regulator of disrupted pathways involved in histone modification and cell cycle regulation. However, its function in UVB-treated HLECs remains unknown.

The promoting effect of the POU3F2/METTL16/PFKM cascade on glycolysis and tumorigenesis of hepatocellular carcinoma: Role of the POU3F2/METTL16/PFKM axis in HCC.

Introduction And Objectives: Deregulation of mA methylation, the most prevailing RNA modification, participates in cancer pathogenesis. METTL16, an atypical methyltransferase, functions as a pro-tumorigenic factor in hepatocellular carcinoma (HCC). Here, we explored the action of METTL16 on HCC glycolysis and the associated mechanism.

Evolutionary insights and expression patterns of sex-related gene families in the zig-zag eel Mastacembelus armatus.

The zig-zag eel exhibits both sexual dimorphism and sex reversal, making it crucial to understand the mechanisms of sex determination and differentiation. Additionally, the wild populations of the zig-zag eel are significantly declining, emphasizing the need for urgent conservation efforts. In this study, we identified 7 Dmrt, 62 HMG-box, and 73 TGF-β family members in the zig-zag eel genome.

Dioxin-like effects of an emerging contaminant 1,3,6,8-tetrabromocarbazole on the myogenic differentiation of mouse C2C12 cells.

1,3,6,8-Tetrabromocarbazole (1368-BCZ) has been proposed as an emerging environmental contaminant which has aryl hydrocarbon receptor (AhR) activating properties analogous to those of dioxins. Skeletal muscle development is a critical target of dioxin toxicity. However, the impact of 1368-BCZ on muscle development is inadequately understood.

The synthesis of an iron tailings-based geopolymer with synergistic electromagnetic wave consumption property.

In this study, combination of wave absorption materials with different loss mechanisms are added into iron ore tailings-blast furnace slag (IOT-BFS) based geopolymers. The employed materials are hollow glass microsphere (HGM), carbon nanotubes (CNT) and carbonyl iron powder (CIP). Microstructures of the geopolymers are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and concrete porous structure analyzer.

Development of a sodium hyaluronate-enriched therapeutic formulation with stevia glycoside and mogroside V for the comprehensive management of diabetes and its complications.

Diabetes prevalence continues to increase as a result of people's increasing sugar intake. Diabetes mellitus and its complications (dry skin, constipation, depression, and dental caries), as well as the prohibition of sweets ingestion, seriously affect patients' physical and mental health. Therefore, it is crucial to develop a long-term food for special medical purposes (FSMP) that aids in managing diabetes and its complications.

Soy protein isolate/dextran glycation conjugates: Fabrication through ultrasound-assisted cyclic continuous reaction and their applications as carriers of anthocyanins.

The precise control of browning and enhancement of Maillard reaction kinetics to improve the surface functionality and nutrient encapsulation efficiency of soy proteins remains a significant challenge. This research presents an ultrasound-assisted cyclic reaction method (1-7 cycles) to synthesize soy protein isolate/dextran (SPI/D) conjugates with enhanced grafting degree and functionality during the Maillard reaction. The technique significantly increased the grafting degree to 65.

A novel emulsifier for Pickering emulsion composed of whey protein and OSA-pectin loaded with Monascus pigments.

Protein-polysaccharide complex carrier can solve the problem of insufficient stability of Monascus pigments (MPs), a kind of natural pigments, against heat and light. It also has the function to stabilize Pickering emulsion (PE) that can be used as fat replacer in meat products. In this study, heat denatured whey protein (HWP) and pectin modified by octenyl succinic anhydride (OSA-pectin) were prepared into complex by adding Ca loaded with MPs.

Lignin fractionation and condensation in aromatic-additive-assisted acidic pretreatment and their influence on lignin's effect on the enzymatic hydrolysis.

Factors influencing inhibition of lignin on the enzymatic hydrolysis have not been fully elucidated. This study aims to elucidate the effects of lignin fractionation and condensation on its inhibition on enzymatic hydrolysis in aromatic-additive-assisted acidic pretreatment using 2-naphthol (2 N), 2-naphthol-7-sulfonate (NS), and resorcinol (RS). Through simulation reactions of pretreatment and physiochemical analyses of ethanol-extractable lignins (ELs) and cellulolytic enzyme lignins (CELs) from pretreatment, it was observed that 2 N addition in the acidic pretreatment could suppress lignin condensation.

Ascorbic acid transporter MmSLC23A2 functions to inhibit apoptosis via ROS scavenging in hard clam (Mercenaria mercenaria) under acute hypo-salinity stress.

Solute carrier family 23 (SLC23) mediates cellular uptake of ascorbic acid, a crucial antioxidant protecting organisms against oxidative stress. Despite advances in understanding SLC23 in mammals, its physiological roles in bivalves remain poorly understood. Notably, euryhaline bivalves exhibit a significant expansion and positive selection of SLC23, highlighting the need for deeper investigation.

Solvent induced method for preparation of multi-advantage carrageenan films.

Carrageenan has good film-forming characteristics, but it is difficult to simultaneously improve its multiple performances, such as water-resistance, light transmittance and thermal stability. In this study, multi-advantage composite films were prepared by iota-carrageenan and quaternary ammonium surfactants according to solvent induced method. The weight change, FTIR and thermogravimetric analyses of the films before and after solvent inducement indicated that the inorganic counterions of iota-carrageenan were replaced by quaternary ammonium ions.

Deficiency of hepatokine orosomucoid1 aggravates NAFLD progression in mice.

Orosomucoid (ORM) is an important hepatokine that regulates metabolism. Previous report showed that isoform ORM2 but not ORM1 could downregulate lipogenic genes and ameliorate hepatic steatosis in obese mice, thereby categorizing ORM2 as a promising candidate for therapeutic intervention in nonalcoholic fatty liver disease (NAFLD). However, our previous studies found that mice lacking ORM1 gradually developed an obese phenotype with severe hepatic steatosis at the age of 24 weeks.

Serinc2 antagonizes pressure overload-induced cardiac hypertrophy via regulating the amino acid/mTORC1 signaling pathway.

Background: Cardiac hypertrophy is characterized by the upregulation of fetal genes, increased protein synthesis, and enlargement of cardiac myocytes. The mechanistic target of rapamycin complex 1 (mTORC1), which responds to fluctuations in cellular nutrient and energy levels, plays a pivotal role in regulating protein synthesis and cellular growth. While attempts to inhibit mTORC1 activity, such as through the application of rapamycin and its analogs, have demonstrated limited efficacy, further investigation is warranted.

Biomimetic reactive oxygen/nitrogen nanoscavengers inhibit "ferroptosis storm" and modulate immune targeting for acute kidney injury.

Cisplatin (Cis), a potent chemotherapeutic agent, often causes acute kidney injury (AKI), limiting its clinical efficacy. RONS flares at the AKI site are a key factor in its progression. In this study, leveraging the advantages of cell membrane-coated biomimetic nanocarriers, we developed a multifunctional biomimetic nanodelivery system nano-RONS-sacrificial agent for AKI treatment.

Combating cisplatin-resistant lung cancer using a coiled-coil peptides modified membrane fused drug delivery system.

Drug resistance to chemotherapy in treating cancers becomes an increasingly serious challenge, which leads to treatment failure and poor patient survival. Drug-resistant cancer cells normally reduce intracellular accumulation of drugs by controlling drug uptake and promoting drug efflux, which severely limits the efficacy of chemotherapy. To overcome this problem, a membrane fused drug delivery system (MF-DDS) was constructed to treat cisplatin (DDP)-resistant lung cancer (A549-DDP) by delivering DDP via membrane fusion using a complementary coiled-coil forming peptides (CPK/CPE).

Construction and verification of an infectious cDNA clone of encephalomyocarditis virus from pigs.

In this study, a novel Encephalomyocarditis virus (EMCV) reverse genetic operating system was developed utilizing CMV promoters, enabling EMCV genome expression under the transcriptional control of the CMV immediate early promoter and BGH polyA transcriptional-termination signal. The full-length cDNA of EMCV BJC3 was ligated to the pRK5 vector, incorporating the CMV eukaryotic promoter sequence, resulting in the construction of recombinant plasmid EMCV (pEMCV). Subsequently, the recombinant plasmid was transfected into BHK-21 cells to generate the rescue virus.

Novel insights into seasonal airborne bacterial interactions and potential threats to human health in a northwest city, China.

Bioaerosols significantly influence air quality and human health. This study investigated the diversity, structure, and interaction of bacterial communities in particulate matter (PM) across four seasons in Xi'an. The results revealed that operational taxonomic units (OTUs) were the highest in autumn, reaching levels comparable to those in winter, but were 3.

Microplastics and Dechlorane Plus co-exposure amplifies their impacts on soybean plant.

The co-existence of microplastics (MPs) and organic pollutants on agricultural ecosystems pose potential implications for both food safety and environmental integrity. The combined effects of MPs with Dechlorane Plus (DP), a newly listed banned flame retardant, remain unknown. This study explores the biological responses of soybean plants to exposure from polyethylene (PE) and polyvinyl chloride (PVC) MPs and DP.

Utilizing zebrafish models to elucidate mechanisms and develop therapies for skeletal muscle atrophy.

Skeletal muscle atrophy, resulting from an imbalance in muscle protein synthesis and degradation, compromises muscle quality and function, imposing significant burdens on movement and metabolic stability. Animal models are crucial for understanding the mechanisms of skeletal muscle atrophy and developing clinical prevention and treatment strategies. Zebrafish, as small aquatic vertebrates, exhibit high genetic homology with humans and offer advantages such as rapid reproduction, development, and transparent embryos.