Calnexin promotes glioblastoma progression by inducing protective mitophagy through the MEK/ERK/BNIP3 pathway.

Glioblastoma multiforme (GBM), one of the most malignant tumors of the central nervous system, has a poor prognosis, mainly because of its high recurrence caused by the rapid development of drug resistance to postoperative chemotherapy. Although macroautophagy/autophagy is believed to be a fundamental factor in tumor survival during chemotherapy, there is still a lack of autophagy biomarkers for predicting patient prognosis and chemotherapeutic efficacy in clinical practice. We combined transcriptomic and single-cell sequencing data to identify differentially expressed autophagy-related genes in gliomas.

A Pathway-Level Information ExtractoR (PLIER) framework to gain mechanistic insights into obesity in Down syndrome.

Down syndrome (DS), caused by the triplication of chromosome 21 (T21), is a prevalent genetic disorder with a higher incidence of obesity. Traditional approaches have struggled to differentiate T21-specific molecular dysregulation from general obesity-related processes. This study introduces the omni-PLIER framework, combining the Pathway-Level Information ExtractoR (PLIER) with the omnigenic model, to uncover molecular mechanisms underlying obesity in DS.

Domestic violence and depression among Chinese adolescents: The role of self-evaluation.

Background: Depression of adolescents seriously affects their mental health as well as the formation and development of sound personality. According to Family Systems Theory, individuals' mental health is closely related with external (e.g.

A hybrid constrained continuous optimization approach for optimal causal discovery from biological data.

Motivation: Understanding causal effects is a fundamental goal of science and underpins our ability to make accurate predictions in unseen settings and conditions. While direct experimentation is the gold standard for measuring and validating causal effects, the field of causal graph theory offers a tantalizing alternative: extracting causal insights from observational data. Theoretical analysis has shown that this is indeed possible, given a large dataset and if certain conditions are met.

A neutral polysaccharide from Persicaria hydropiper (L.) Spach ameliorates lipopolysaccharide-induced intestinal barrier injury via regulating the gut microbiota and modulating AKT/PI3K/mTOR and MAPK signaling pathways.

Ethnopharmacological Relevance: Persicaria hydropiper (L.) Spach, a herb that is prevalent across Asia and Europe, finds utility as both a culinary ingredient and medicinal herb. In China, P.

How does national development zone policy affect carbon emissions in China? New evidence from a quasi-natural experiment.

The expansion of China's development zones has made great contributions to economic development, as well as provided practical guidance for other developing countries to implement development zone policies. However, in the context of global advocacy of low carbon, literature about how the development zone policy affect carbon emissions is poor, especially in China at the urban level. Therefore, this study takes China's development zone policy as a quasi-natural experiment, using the panel data of 285 cities in China from 2003 to 2020, and adopting the DID model to analyze its impact on carbon emissions.

RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats.

Background: MicroRNAs (miRNAs) are a family of short non-coding RNA molecules and play important roles in various biological processes. However, knowledge of the expression profiles and function of miRNAs on the regulation of brown adipose tissue (BAT) thermogenesis remains largely unknown.

Results: In this study, we found that brown adipose tissue (BAT) existed within the perirenal fat at 1 day after birth (D1) and transferred into white adipose tissue (WAT) at 30 days after birth (D30) by UCP1 protein expression and immunohistochemistry analysis.

Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome Activation of Autophagy.

Oridonin (Ori) is a diterpene compound that has multiple biological properties. Here, our study was conducted to observe the therapeutic effect of Ori on depression as well as to uncover the mechanism. Lipopolysaccharide (LPS)-induced depression models were established both in C57BL/6 mice and primary astrocytes, which were treated with Ori, autophagy agonist Rapamycin (Rap) and autophagy inhibitor 3-Methyladenine (3-MA).

[Spatiotemporal variations of ecosystem service values and landscape ecological risk and their correlation in forest area of Loess Plateau, China:A case study of Ziwuling region.].

Although the awareness of ecosystem services provided by natural landscape is increa-sing, few studies integrate ecosystem services value (ESV) into ecological risk management and its control. With Ziwuling region as an example, we carried out the gridding resampling of landscape pattern types in the forest area of Ziwuling region in 1980, 1990, 2000, 2010 and 2017. We quantified the ESV and landscape ecological risk and their spatiotemporal variations, based on the 2.

Expression of NOD1 and downstream factors in placenta, fetal membrane and plasma from pregnancies with premature rupture of membranes and their significance.

Objective: To investigate the expression and significance of NOD1 (nucleotide oligomerization domain 1) and its downstream factors in placenta, fetal membrane and plasma of pregnancies with premature rupture of membranes (PROM).

Methods: 60 cases of PROM pregnancies were recruited, including 30 cases of preterm premature rupture of membranes (< 37 weeks) and 30 cases of mature premature rupture of membranes (≥ 37 weeks); 30 healthy pregnancies in the same period were selected as a control group (gestational weeks ≥ 37 weeks). Western blot was used to detect the expression of NOD1, receptor interacting protein 2 (RIP2) and nuclear factor-κB (NF-κB) in placenta and fetal membrane tissues of the three groups.

Genome-Wide Identification and Characterization of Long Noncoding RNAs of Brown to White Adipose Tissue Transformation in Goats.

Long noncoding RNAs (lncRNAs) play an important role in the thermogenesis and energy storage of brown adipose tissue (BAT). However, knowledge of the cellular transition from BAT to white adipose tissue (WAT) and the potential role of lncRNAs in goat adipose tissue remains largely unknown. In this study, we analyzed the transformation from BAT to WAT using histological and uncoupling protein 1 () gene analyses.

Glycogen synthase kinase 3β (GSK3β) regulates the expression of MyHC2a in goat skeletal muscle satellite cells (SMSCs).

Glycogen synthase kinase beta (GSK3β) plays an important role in skeletal muscle growth, regeneration, and repair. However, the mechanism of GSK3β regulating MyHC2a expression is currently not clear. In this study, GSK3β inhibition promoted skeletal muscle satellite cells (SMSCs) differentiation and increased expression of MyoD, MyoG, MyHC1, and MyHC2a genes.

Association of Mannose-binding Lectin Polymorphisms with Tuberculosis Susceptibility among Chinese.

Tuberculosis (TB) is caused by infection of Mycobacterium tuberculosis. Host genetic variability is an important determinant of the risk of developing TB in humans. Although the association between MBL2 polymorphisms and TB has been studied in various populations, the results are controversial.

p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.

PAK1 inhibitors are known to markedly improve social and cognitive function in several animal models of brain disorders, including autism, but the underlying mechanisms remain elusive. We show here that disruption of PAK1 in mice suppresses inhibitory neurotransmission through an increase in tonic, but not phasic, secretion of endocannabinoids (eCB). Consistently, we found elevated levels of anandamide (AEA), but not 2-arachidonoylglycerol (2-AG) following PAK1 disruption.

Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization.

Neuroligin (NLG) 1 is important for synapse development and function, but the underlying mechanisms remain unclear. It is known that at least some aspects of NLG1 function are independent of the presynaptic neurexin, suggesting that the C-terminal domain (CTD) of NLG1 may be sufficient for synaptic regulation. In addition, NLG1 is subjected to activity-dependent proteolytic cleavage, generating a cytosolic CTD fragment, but the significance of this process remains unknown.

Exceedingly expanded retroauricular flaps for microtia reconstruction.

Background: The authors propose here a new strategy to obtain exceedingly expanded retroauricular mastoid skin for sufficient coverage of the three-dimensional autogenous costal cartilage framework generally used in auricular reconstruction surgery. From February 2000 to September 2009, 42 microtia reconstructions were performed using this new strategy.

Methods: Auricular reconstruction was performed in three surgical stages.

5-Hydr-oxy-1-(3-hydr-oxy-2-naphtho-yl)-3,5-dimethyl-2-pyrazoline.

In the title mol-ecule, C(16)H(16)N(2)O(3), intra-molecular O-H⋯O hydrogen bonds influence the mol-ecular conformation. Inter-molecular O-H⋯O hydrogen bonds [O⋯O = 2.922 (2) Å] link the mol-ecules into centrosymmetric dimers.

N,N'-disalicyloylhydrazine.

The approximately planar mol-ecule of the title compound, C(14)H(12)N(2)O(4), is centrosymmetric and has an E configuration with respect to the N-N bond. This compound adopts the ketoamine form with C=O and C-N distances of 1.233 (3) and 1.