Co-metabolism of Norfloxacin by Chlorella pyrenoidosa: Carbon source effects, biotransformation mechanisms, and key driving genes.

Co-metabolism with appropriate carbon sources has been demonstrated to effectively enhance the removal of ubiquitous recalcitrant micropollutant by microalgae. However, the specific impacts of carbon sources on the co-metabolism of antibiotics by microalgae remain insufficiently explored. In this study, transcriptomics, gene network analysis, extracellular polymeric substances (EPS), and enzymatic activity involved in co-metabolic pathways of norfloxacin (NFX), were systematically evaluated to investigate the underlying biological mechanisms involved in NFX co-metabolism by Chlorella pyrenoidosa.

Environmental contamination and risks of organic UV filters: Source, discharge, analytical methods and implications for ecological and human health.

Organic Ultraviolet Filters (OUVFs), commonly used in sunscreens, cosmetics and industrial products to prevent ultraviolet radiation damage, are increasingly detected in the environment due to their widespread use and persistence. This has raised concerns over their toxicity and environmental impact, leading to the classification of OUVF 2-(2H-Benzotriazol-2-yl)-4,6-ditertpentylphenol (UV-328) as a persistent organic pollutant under the Stockholm Convention in 2023. In this review, current knowledge on the usage, discharge and environmental contamination of OUVFs is briefly discussed.

TIRPnet: Risk prediction of traditional Chinese medicine ingredients based on a deep neural network.

Ethnopharmacological Relevance: Traditional Chinese medicine (TCM) has a history of over 3000 years of medical practice. Due to the complex ingredients and unclear pharmacological mechanism of TCM, it is very difficult to predict its risks. With the increase in the number and severity of spontaneous reports of adverse drug reactions (ADRs) of TCM, its safety has received widespread attention.

Comparison of the Radiographic and Clinical Outcome Changes in Patients With Spinal Deformity Before and After Stretching DanceExercise: A Systematic Review and Meta-Analysis.

Objective: This study aimed to compare radiographic and clinical outcomes of scoliosis and thoracic hyperkyphosis before and after stretching-based exercises.

Methods: Embase, PubMed, Cochrane Library, Web of Science, and Scopus databases were comprehensively searched for relevant studies from their inception to June 2022. Radiographic outcomes, including the Cobb angle of the main curve and thoracic kyphosis, and clinical outcomes, including the angle of trunk rotation (ATR), chest expansion, Numeric Rating Scale (NRS), and Scoliosis Research Society-22 Patient Questionnaire (SRS-22), were extracted.

The relationship of α-hydroxybutyrate dehydrogenase with 1-year outcomes in patients with intracerebral hemorrhage: A retrospective study.

Background And Aims: Cardiac enzymes are recognized as a valuable tool for predicting the prognosis of various cardiovascular diseases. The prognostic value of alpha-hydroxybutyrate dehydrogenase (α-HBDH) in patients with intracerebral hemorrhage (ICH) was ambiguous and not evaluated.

Methods: Two hundred and thirteen Chinese patients with ICH participated in the study from December 2018 to December 2019.

GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in .

is a widely distributed marine bacterium that is a threat to the aquaculture industry as well as human health. Evidence has revealed critical roles for small RNAs (sRNAs) in bacterial physiology and cellular processes by modulating gene expression post-transcriptionally. GcvB is one of the most conserved sRNAs that is regarded as the master regulator of amino acid uptake and metabolism in a wide range of Gram-negative bacteria.

Development and validation of a simultaneous method for the analysis of benzothiazoles and organic ultraviolet filters in various environmental matrices by GC-MS/MS.

The presence of benzothiazoles (BTHs) and organic ultraviolet filters (UV filters) in aquatic ecosystems has emerged as a significant environmental issue, requiring urgent and efficient determination methods. A new, rapid, and sensitive determination method using gas chromatography triple quadrupole mass spectrometer (GC-MS/MS) was developed for the simultaneous extraction and analysis of 10 commonly used BTHs and 10 organic UV filters in surface water, wastewater, sediment, and sludge. For aqueous samples, solid-phase extraction (SPE) method was employed with optimizing of SPE cartridge type, pH, and elution solvent.

Predictive value of the apolipoprotein B/A1 ratio in intracerebral hemorrhage outcomes.

Background And Aims: The apolipoprotein B (apoB)/apolipoprotein A1 (apoA1) ratio is a key indicator in predicting future cardiovascular outcomes. However, it is still unclear whether the ratio of apoB/apoA1 is a better predictor of the outcomes after intracerebral hemorrhage (ICH). Therefore, we aimed to assess the relationships between the ratio of apoB/apoA1 and functional outcomes, all-cause mortality, and stroke recurrence in ICH patients.

CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in .

, like other species, is a widely distributed marine bacterium that is able to outcompete other species in variable niches where diverse organic matters are supplied. However, it remains unclear how these cells sense and adjust metabolic flux in response to the changing environment. CsrA is a conserved RNA-binding protein that modulates critical cellular processes such as growth ability, central metabolism, virulence, and the stress response in -proteobacteria.

Molecular cloning, expression and functional analysis of NF-kB1 p105 from sea cucumber Holothuria leucospilota.

The nuclear factor-κB (NF-κB) family is evolutionary conserved and plays key roles in the regulation of numerous basic cellular processes. In this study, a sea cucumber Holothuria leucospilota NF-κB1 p105 named HLp105 was first obtained. The full-length cDNA of HLp105 is 6564 bp long, with a 219 bp 5' untranslated region (UTR), a 2979 bp 3' UTR, and a 3366 bp open reading frame (ORF) encoding for 1121 amino acids with a deduced molecular weight of 123.

Risk Factors of Recurrent Ischemic Events after Acute Noncardiogenic Ischemic Stroke.

Background: A considerable proportion of acute noncardiogenic ischemic stroke patients continue to experience recurrent ischemic events after standard therapy.

Aim: We aimed to identify risk factors for recurrent ischemic event prediction at an early stage.

Methods: 286 non-cardioembolic ischemic stroke patients with the onset of symptoms within 24 hours were enrolled.

Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells.

Curcumin is a key polyphenolic curcuminoid extracted from the root of turmeric rhizome Curcuma longa Linn, which is a frequently used Chinese herb for the treatment of cancer. The aim of the present study was to investigate the mechanism of the inhibitory effects of curcumin on nude mice with lung cancer A549 cell subsets side population (SP) and non-SP (NSP) cells. BALB/c mice were subcutaneously injected with the tumor cells of A549 SP or NSP subsets consisting of 1×10 cells/l (0.

Two-stage fermentation for 2-Ketogluconic acid production by Klebsiella pneumoniae.

2-Ketogluconic acid production by Klebsiella pneumoniae is a pH-dependent process, strictly proceeding under acidic conditions. Unfortunately, cell growth is inhibited by acidic conditions, resulting in low productivity of 2-ketogluconic acid. To overcome this deficiency, a two-stage fermentation strategy was exploited in the current study.

IL-4 induces a suppressive IL-10-producing CD8+ T cell population via a Cdkn2a-dependent mechanism.

CD8(+) T cells play an important role in immune regulation and effective immune responses against tumor cells, viral infection, and intracellular pathogens. In this report, using tiger or 10BiT mice, we defined a population of IL-10-producing CD8(+) T cells that were induced by IL-4. These IL-10(+)CD8(+) T cells possessed a strong inhibitory effect on the CD4(+) T cell proliferation in an IL-10-dependent and cell contact-dependent fashion.

A novel method for signal detection of adverse drug reactions based on proportional reporting ratios.

Objective: The proportional reporting ratio (PRR) is a statistical method for signal detection of adverse drug reactions (ADRs) based on unbalanced proportions. Although effective, this method only takes into account the proportional relation based on target adverse reactions and ignores the relation between a given ADR and the others for the same drug. Therefore, it is necessary to improve the calculation deviation in PRR.