We construct N=1 supersymmetric (SUSY) field theory in 4+2 dimensions compatible with the theoretical framework of two-time (2T) physics and its gauge symmetries. The fields are arranged into 4+2 dimensional chiral and vector supermultiplets, and their interactions are uniquely fixed by SUSY and 2T physics gauge symmetries. In a particular gauge the 4+2 theory reduces to ordinary supersymmetric field theory in 3+1 dimensions without any Kaluza-Klein remnants, but with some additional constraints in 3+1 dimensions of interesting phenomenological relevance.
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