Publications by authors named "Yuege Huang"
Article Synopsis
- Baculovirus is a virus that infects insects and is used in labs and industries for protein production and gene therapy, but its ability to enter mammalian cells is not fully understood.
- A study used a CRISPR screening method to find host factors that help baculovirus infect human cells, identifying EXTL3 and NPC1 as key proteins involved in this process.
- EXTL3 aids in virus attachment through heparan sulfate biosynthesis, while NPC1 facilitates viral entry by promoting membrane fusion and escape from endosomes; disruption of NPC1 in mice significantly reduced baculovirus transduction in their liver.
In humans, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can cause medical complications across various tissues and organs. Despite the advances to understanding the pathogenesis of SARS-CoV-2, its tissue tropism and interactions with host cells have not been fully understood. Existing clinical data have revealed disordered calcium and phosphorus metabolism in Coronavirus Disease 2019 (COVID-19) patients, suggesting possible infection or damage in the human skeleton system by SARS-CoV-2.
View Article and Find Full Text PDFBackground: Rhinoviruses (RVs) cause more than half of common colds and, in some cases, more severe diseases. Functional genomics analyses of RVs using siRNA or genome-wide CRISPR screen uncovered a limited set of host factors, few of which have proven clinical relevance.
Results: Herein, we systematically compare genome-wide CRISPR screen and surface protein-focused CRISPR screen, referred to as surfaceome CRISPR screen, for their efficiencies in identifying RV host factors.