An effective immobilization of β-glucosidases by partly cross-linking enzyme aggregates.

Enzyme immobilization provides ideal operating conditions for enzymes stabilization and sustainable recycling. In this work, as a kind of clay material, montmorillonite (MTL) was chosen for immobilizing the β-glucosidase extracted from . The immobilized β-glucosidase via partly cross-linking enzyme aggregates (pCLEAs) formed by self-catalysis provided biocatalysts with satisfactory thermal and pH stability.

Adsorption of phenol from water by N-butylimidazolium functionalized strongly basic anion exchange resin.

N-butylimidazolium functionalized strongly basic anion exchange resin with Cl(-) anion (MCl) was prepared by anchoring N-butylimidazole onto chloromethylated macroporous styrene-divinylbenzene (St-DVB) copolymer. The adsorption performances of phenol on MCl were studied using the batch technique at acidic and alkaline pH. The studies showed that phenol can be effectively removed at both acidic and alkaline pH.

[Biodegradation of [omim][PF6]].

Acclimatized the activated sludge with [omim][PF6] which was used as the C, N, P resources for microbe, the biodegradation ratio of [omim][PF6] with different inoculants which were acclimatized or not was investigated. And the influence of appending an ester in the side chain of the imidazolium cation on the biodegradation of [omim][PF6] was discussed in the same conditions. The biodegradation pathways of [omim]+ were analyzed.

Malignant angiomyolipoma in the liver: a case report with pathological and molecular analysis.

Malignant angiomyolipoma (AML) of the liver is rare. We report a case of AML with malignant transformation and metastases. A 30-year-old man had developed giant hepatic masses.

Separation of ternary systems of hydrophilic ionic liquid with miscible organic compounds by RPLC with refractive index detection.

A rapid and simple analytical method was developed for the simultaneous and quantitative determination and separation of hydrophilic imidazolium ionic liquids (ILs) (1-butyl-3-methylimidazolium chloride, [C(4)mim]Cl; 1-hexyl-3-methylimidazolium chloride, [C(6)mim]Cl; 1-octyl-3-methylimidazolium chloride, [C(8)mim]Cl; 1-allyl-3-methylimidazolium chloride, [Amim]Cl; or 1-allyl-3-methylimidazolium bromide, [Amim]Br) with miscible ethyl acetate and EtOH and their mixtures using reverse phase liquid chromatography coupled with refractive index detection (RPLC-RI). The influence of 60 to 100% (volume percentage) methanol in the mobile phase on the IL systems ([C(4)mim]Cl, [C(6)mim]Cl, [C(8)mim]Cl, [Amim]Br, or [Amim]Cl)-ethyl acetate-EtOH was investigated. The optimum mobile phase for the system [C(8)mim]Cl-ethyl acetate-EtOH, [C(4)mim]Cl-ethyl acetate-EtOH, [Amim]Br-ethyl acetate-EtOH and [Amim]Cl-ethyl acetate-EtOH was methanol/water (60:40, v/v), and methanol/water (70:30, v/v) for [C(6)mim]Cl-ethyl acetate-EtOH.

Synthesis and evaluation of Gd-DTPA-labeled arabinogalactans as potential MRI contrast agents.

Arabinogalactan derivatives conjugated with gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) by ethylenediamine (Gd-DTPA-CMAG-A2) or hexylamine (Gd-DTPA-CMAG-A6) have been synthesized and characterized by means of Fourier transform infrared spectra (FTIR), 13C nuclear magnetic resonance (13C NMR), size exclusion chromatography (SEC), and inductively coupled plasma atomic emission spectrometry (ICP-AES). Relaxivity studies showed that arabinogalactan-bound complexes possessed higher relaxation effectiveness compared with the clinically used Gd-DTPA, and the influence of the spacer arm lengths on the T1 relaxivities was studied. Their stability was investigated by competition study with Ca2+, EDTA, and DTPA.