Aim: To determine insulin and its related substances in insulin powder for inhalation by reversed phase high performance liquid chromatography method.
Methods: The initial mobile phase was solution A (0.1% trifluoroacetic acid-acetonitril 70:30) and changed to solution B (0.
Aim: To observe the hypoglycemic efficacy of pulmonary delivery of insulin in dry powder aerosol form.
Methods: Insulin dry powder, made of insulin and other proper materials, was insufflated in rat lung from an incision in the throat. Meanwhile, insulin injection was administered to other rats.