[Induction of mouse embryonic stem cells forming odontoblast-like cells by co-culture with pulp fibroblast].

Purpose: To investigate the methods of induction of mouse embryonic stem cells to differentiate into odontoblast-like cells by co-culture with pulp fibroblast.

Methods: By suspension culture, embryonic stem cells were induced to form embryoid bodies. Then the cells from embryoid bodies were co-cultured with pulp fibroblast in Transwell system for differentiation toward odontoblast-like cells.

[Primary culture and identification of mouse odontoblast-like cells].

Purpose: To culture primary mouse odontoblast and to provide a base for study on inducing ES cells to odontoblast.

Methods: Lower incisor germs were removed from 1-week-old mouse. The dental papillae were isolated in microscope, and the dental papillae cells were dispersed using 0.