Effect of endogenous insulin-like growth factor and stem cell factor on diabetic colonic dysmotility.

Aim: To investigate whether the reduction of stem cell factor (SCF) is mediated by decreased endogenous insulin-like growth factor (IGF)-1 in diabetic rat colon smooth muscle.

Methods: Sixteen Sprague-Dawley rats were randomly divided into two groups: control group and streptozotocin-induced diabetic group. After 8 wk of streptozotocin administration, colonic motility function and contractility of circular muscle strips were measured.

[Insulin-like growth factor 1 regulates expression of stem cell factor through ERKMAPK signaling pathway in gastric smooth muscle cell].

Objective: To explore the effect and the intracellular signal transduction pathway of insulin-like growth factor 1 (IGF-1) on the expression of stem cell factor (SCF) in gastric smooth muscle cells (SMC).

Methods: Gastric SMC from SD rats were cultured by enzymolysis and identified by α-actin immunofluorescence methods. Western blot and quantitative reverse transcription-polymerase chain reaction were used to examine the expression of SCF in gastric SMC:(1) The level of SCF after gastric SMC were cultured with IGF-1.

T300A polymorphism of ATG16L1 and susceptibility to inflammatory bowel diseases: a meta-analysis.

Aim: To evaluate the association of the autophagy-related 16-like 1 (ATG16L1) T300A polymorphism (rs2241880) with predisposition to inflammatory bowel diseases (IBD) by means of meta-analysis.

Methods: Publications addressing the relationship between rs2241880/T300A polymorphism of ATG16L1 and Crohn's disease (CD) and ulcerative colitis (UC) were selected from the MEDLINE and EMBASE databases. To make direct comparisons between the data collected in these studies, the individual authors were contacted when necessary to generate a standardized set of data from these studies.