Combined computational analysis and cytology show limited depth osteogenic effect on bone defects in negative pressure wound therapy.

The treatment of bone defects remains a clinical challenge. The effect of negative pressure wound therapy (NPWT) on osteogenesis in bone defects has been recognized; however, bone marrow fluid dynamics under negative pressure (NP) remain unknown. In this study, we aimed to examine the marrow fluid mechanics within trabeculae by computational fluid dynamics (CFD), and to verify osteogenic gene expression, osteogenic differentiation to investigate the osteogenic depth under NP.

Do stress fractures induce hypertrophy of the grafted fibula? A report of three cases received free vascularized fibular graft treatment for tibial defects.

The presence of large segmental defects of the diaphyseal bone is challenging for orthopedic surgeons. Free vascularized fibular grafting (FVFG) is considered to be a reliable reconstructive procedure. Stress fractures are a common complication following this surgery, and hypertrophy is the main physiological change of the grafted fibula.

Vascularised greater trochanter bone graft, combined free iliac flap and impaction bone grafting for osteonecrosis of the femoral head.

Purpose: To investigate the curative efficacy of osteonecrosis of the femoral head (ONFH) in a hip-preserving operative approach, by grafting a vascularized greater trochanter flap combined with a free iliac flap, in an attempt to seek an innovative approach for patients who suffered middle to late stage ONFH without total hip arthroplasty (THA) surgery.

Method: Our research included a total of 60 patients (66 hips) who accepted hip-preserving surgery by grafting a vascularized greater trochanter flap combined with a free iliac flap which was tightly filled by hammering because of ONFH (most were Association Research Circulation Osseous (ARCO) stage III patients) from January, 2006 to December, 2010. A Harris Hip Score was obtained during follow-ups, evaluating the clinical efficacy, X-rays were taken regularly for image assessing, and the SF-36 scale was used for estimating quality of life.

[Experimental study of directional differentiation of bone mesenchymal stem cells (BMSCs) to osteoblasts guided by serum containing cistanche deserticola].

Objective: To study directional differentiation of BMSCs guided by Desert living Cistanche (Herba Cistanches) which invigorates the kidney.

Methods: Primary BMSCs were obtained by whole bone marrow culture and subcultured to the fourth generation by trypsin digestion, and than inoculated into two six-well plates at 5 x 10(6) cells per milliliter, all the plates were divided into three groups as blank group, Dexamethasone (DXM) group and Herba Cistanches group, three wells in each group, medium were changed at day 2. The blank group were changed with L-DMEM containing 10% FBS.

[Human osteoblast-like cells OS-732 intervened with alcohol and treated with liuweidihuang pill medicated serum].

Objective: To investigate the mechanism of osteonecrosis of the femoral head caused by alcohol and the therapeutic effects of the method of invigorating the kidney.

Methods: The osteoblast-like cells were intervened with 0.3, 0.

[Treatment for ipsilateral fractures of the femoral neck and shaft].

Objective: To investigate the curative effect of different internal fixation for ipsilateral fractures of the femoral neck and shaft.

Methods: By retrospective study of 27 patients who sustained ipsilateral femoral shaft and neck fractures from June 1993 to March 2004. There were 22 male and 5 female, with an average age of 35 years (range in 14 to 65 years).

[Effects of Shengmai Chenggu capsule on proliferation and vascular endothelial growth factor secretion of vascular endothelial cells].

Objective: To study the effect of the serum of rabbits fed with Shengmai Chenggu capsule (SMCGC), a preparation of traditional Chinese medicine, on the proliferation and vascular endothelial growth factor (VEGF) secretion of cultured ECV304 cells.

Methods: The drug-containing serum from rabbits with intragastric administration of SMCGC at different doses was obtained for treatment of ECV304 cells cultured in vitro, with the cells treated with blank serum and ligustrazine serving as the control. The proliferation of ECV304 cells was detected by MTT assay and VEGF concentration in the culture medium of the cells measured by means of ELISA.