In the present study, both electron spin resonance (ESR) and chemical detection confirmed that lutein [extracted from alfalfa (Medicago sativa L.)], the most abundant xanthophyll in thylakoids of chloroplasts, could serve as an antioxidant to scavenge reactive oxygen species (ROS) in vitro. Lutein exhibited a greater capacity for scavenging hydroxyl (OH) and superoxide (O) radicals than β-carotene at the same concentration, whereas the opposite trend was observed in the capacity for scavenging singlet oxygen (O).
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