lncRNA GAS5 regulates myocardial infarction by targeting the miR-525-5p/CALM2 axis.

Long noncoding RNAs (lncRNAs) play critical roles in the pathogenesis of cardiovascular diseases, especially in myocardial infarction (MI). However, the underlying molecular mechanism of how lncRNA involves and affect MI still remains unclear. This study aimed to investigate the expression of lncRNA growth arrest-specific transcript 5 (GAS5) and its effects on myocardial cells' proliferation, cell cycle, and apoptosis.

Relationship between abnormal vagus nerve tension and basal ganglia cerebral infarction induced paroxysmal atrial fibrillation.

Objective: To investigate the relationship between basal ganglia cerebral infarction and paroxysmal atrial fibrillation (PAF) caused by abnormal vagus nerve tension.

Methods: A total of 1483 cases of elder patients with cerebral infarction who received head CT or MRI examination during the period were enrolled, including 830 male and 613 female, with the average age as 78 years. These cases were divided into basal infarction ganglia group (n = 1045) and non-basal ganglia infarction group (n = 438) according to the anatomic site of cerebral infarction.

Prevention of Atrial Fibrillation by Using Sarcoplasmic Reticulum Calcium ATPase Pump Overexpression in a Rabbit Model of Rapid Atrial Pacing.

BACKGROUND Recent research suggests that abnormal Ca2+ handling plays a role in the occurrence and maintenance of atrial fibrillation (AF). Therefore, Ca2+ release and ingestion depend on properties of the ryanodine receptor (RyR) and sarcoplasmic reticulum Ca2+ATPase2a (SERCA2a). This study aimed to detect whether SERCA2a gene overexpression has a preventive effect on atrial fibrillation caused by rapid pacing right atrium.

The expression and functional evidence for voltage-dependent potassium channel Kv1.3 in lymphocytes during aging in spontaneously hypertensive rats.

Aims: Our previous studies showed that expression and functional profile of voltage-dependent potassium channels Kv1.3 were increased in lymphocytes of spontaneously hypertensive rats (SHR) compared to normotensive rats, suggesting a crucial role for lymphocyte Kv1.3 in the development of hypertension.

Glucagon-like peptide-1 protects against cardiac microvascular endothelial cells injured by high glucose.

Objective: To investigate the protective effect of glucagon-like peptid-1 (GLP-1) against cardiac microvascular endothelial cell (CMECs) injured by high glucose.

Methods: CMECs were isolated and cultured. Superoxide assay kit and dihydroethidine (DHE) staining were used to assess oxidative stress.

Low-level vagosympathetic trunk stimulation inhibits atrial fibrillation in a rabbit model of obstructive sleep apnea.

Background: Atrial fibrillation (AF) is highly associated with obstructive sleep apnea (OSA) in which AF is triggered by hyperactivity of the cardiac autonomic nervous system. Previous studies showed that low-level vagosympathetic trunk stimulation (LLVS), at voltages not slowing sinus rate or AV conduction, inhibits AF by suppressing the cardiac autonomic nervous system.

Objective: The purpose of this study was to investigate whether LLVS delivered at the right vagosympathetic trunk suppresses AF in a rabbit model of OSA.

The effects of subclinical hypothyroidism on serum lipid level and TLR4 expression of monocyte in peripheral blood of rats.

Objective: To observe effect of subclinical hypothyroidism (SCH) on serum lipid level and expression of toll-like receptor 4 (TLR4) in rats' peripheral blood mononuclear cells (PBMC).

Methods: Fifty Wistar female rats were divided into three groups: normal control (NC group; n=10), sham group (n=10), and L-T-4 (L-thyroxine) group (n=30, with thyroidectomy, fed with rich-calcium water after operation. 5 weeks later, abdominal subcutaneous injection of L-T-4: 0.

[The changes of cardioelectrical activity of rat with myocardial infarction receiving sarcoplasmic reticulum Ca(2+)-ATPase gene modified bone marrow stem cell transplantation by microelectrode array technology].

Objective: Therapy effects and cardiac electrical activity comparison of bone marrow stem cells (BMSCs) transplantation and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) gene modified BMSCs transplantation after acute myocardial infarction (AMI) in rats.

Methods: Rats with AMI were divided into 4 groups (n = 30) randomly: normal group (n = 6), saline group (control group, n = 8), BMSCs transplantation group (BMSCs group, n = 8) and SERCA2a gene modified BMSCs transplantation group (BMSCs + rAd.SERCA2a group, n = 8).

[Metal stress-induced arrhythmia and thoracic spinal cord 1-5 nerve remodeling and myocardial electrophysiological remodeling in rats].

Objective: The study aimed to investigate the relationship between arrhythmia occurrence and nerve remodeling of thoracic spinal cord 1-5 nerves as well as myocardial electrophysiological remodeling in a metal stress rat model.

Methods: Thirty SD rats (weight 180-250 g) were randomly divided into control group (n = 10), stress group (n = 10) and fluoxetine group (n = 10, 10 mg/kg i.p.

[Efficacy of sequential ablation of sinus atrial node fat pad and atrial ventricular node fat pad on inducibility of atrial fibrillation evoked by vagus trunk stimulation].

Objective: To explore the efficacy of sequential ablation of epicardial fat pad on inducibility of atrial fibrillation (AF) evoked by stimulating vagus trunk.

Methods: Eighteen adult mongrel dogs were randomly divided into 2 groups (n = 9 each): Group A underwent pre-ablation of sinus-atrial node fad pad (SANFP) and subsequent ablation of atria-ventricular node fad pad (AVNFP). Group B underwent pre-ablation of AVNFP and subsequent ablation of SANFP.

[Impact of magnetic field exposure on cardiac autonomic tone and inducibility of atrial fibrillation in dogs].

Objective: To observe the maximal heart rate changes, atrioventricular (A-V) conduction block and atrial fibrillation (AF) inducibility in dogs with vagosympathetic trunk exposed to electromagnetic fields (EMFs).

Methods: The vagosympathetic trunk of adult dogs was separated and exposed to EMFs 0.043 kHz (2.

[Value of aVR lead four steps algorithm on differential diagnosis of wide QRS complex tachycardia].

Objective: The aVR lead four steps is a new algorithm for differential diagnosis of wide QRS complex tachycardia (WCT). The study explores the clinical value of this new algorithm on differential diagnosis of WCT.

Methods: Application of aVR lead four steps to analysis the electrocardiogram of patients with WCT proved by electrophysiological study.

[Nerve remodeling in a canine model of atrial fibrillation induced by 48 hours right atrial pacing].

Objective: To evaluate the nerve remodeling induced by 48 hours right atrial pacing in a canine model.

Methods: Rapid right atrial pacing (600 beats/min) was performed in 6 mongrel dogs of either sex for 48 hours to induce sustained atrial fibrillation (AF). Six dogs without pacing served as controls.

[Microelectrode arrays mapped tissue field action potential duration changes of thoracic spinal cord 1 - 5 nerves and heart in chronic stress rat model].

Objective: To investigate chronic stress induced tissue action potential and pathological changes of thoracic spinal cord 1 - 5 nerves and heart in SD rats.

Methods: SD rats (weighing 180 - 250 g) were randomly divided into depressive group and control group (n = 10 each). Depressive model (unpredicted chronic mild stress) was established according to Gronli's protocol.

Investigation of the alterations in cellular electrophysiology underlying ventricular arrhythmia in dogs with the multiple organ dysfunction syndrome.

Background: Multiple organ dysfunction syndrome (MODS)-specific cellular electrophysiological changes have so far not been reported and it seemed unlikely that they were related to arrhythmogenesis.

Methods And Results: Twelve dogs, weight 12 +/- 2 kg, were divided into a control group (n = 6) and an MODS group (n = 6). MODS lasting for 72 h was induced by the 'two-hit' method in 6 dogs.

[Cardiac field potentials and activation sequence in Langendorff perfused heart, cardiac tissue slices and cultured ventricular myocytes recorded by microelectrode arrays system.].

Objective: To observe field potentials (FPs) and activation sequence at Langendorff perfused guinea pigs heart, SD rat cardiac tissue strips perfused by Tyrode's solution and cultured ventricular cardiomyocytes of suckling mice by microeletrode arrays (MEA) technique.

Method: FPs and activation sequence were recorded from perfused heart, cardiac tissue strips (5 mm x 5 mm) and cultured ventricular cardiomyocytes by MEA.

Results: (1) FPs could be recorded in hearts perfused for 30 to 90 min with a heart rate 90 - 120 beats/min.