[Study on diagnosis of schistosomiasis by ELISA using periodate-treated soluble egg antigen].

Objective: To improve SEA-ELISA, an immunodiagnostic assay for schistosomiasis.

Methods: Soluble egg antigen (SEA) of Schistosoma japonicum was treated with sodium periodate (SP) in order to oxidate its glycosylated epitopes. ELISA using the treated SEA was then performed to detect specific antibodies to SEA in the sera of schistosomiasis patients.

[Fractionation and identification of Schistosoma japonicum adult worm 67kD protein].

Objective: To isolate the specific protein bands which react to sera from rabbits infected with Schistosoma japonicum (Sj) and those immunized with Sj adult worm antigens (AWA) for immunodiagnosis of schistosomiasis.

Methods: AWA was analysed using SDS-PAGE and immunoblotting, and 67 kD protein was fractionated by electrophoretic chromatography.

Results: 67 kD protein which reacted to rabbit sera infected with Sj and immunized with AWA was obtained after the fractionation of AWA through electrophoretic chromatography.