Purification and characterization of a naringinase from Aspergillus aculeatus JMUdb058.

A naringinase from Aspergillus aculeatus JMUdb058 was purified, identified, and characterized. This naringinase had a molecular mass (MW) of 348 kDa and contained four subunits with MWs of 100, 95, 84, and 69 kDa. Mass spectrometric analysis revealed that the three larger subunits were β-D-glucosidases and that the smallest subunit was an α-L-rhamnosidase.

Study on sero-diagnosis of nasopharyngeal carcinoma using a dual antibody test against recombinant Epstein-Barr virus antigens.

Background & Objective: The aim of this study was to optimize a dual-antibody assay for sero-diagnosis of nasopharyngeal carcinoma(NPC) by evaluating 4 Epstein-Barr virus(EBV) antigen-based enzyme-linked immunosorbent assays(ELISAs).

Methods: The serum samples of 57 pretreated NPC patients and 58 apparently healthy adults in Guangzhou were collected. The levels of anti-EBV antibody in the sera were tested by 4 ELISAs, which were developed using fusion proteins of glutathione transferase and EBV specific recombinant antigens, namely, EBNA1-IgA, EBNA1-IgG, Zta-IgA, and Zta-IgG.