Enhanced Two-Photon Fluorescence and Fluorescence Imaging of Novel Probe for Calcium Ion by Self-Assembly with Conjugated Polymer.

The calcium ion (Ca) isa highly versatile intracellular signal messenger regulating many different cellular functions. It is important to design probes with good fluorescence and two-photon (TP) active cross-sections (Φ) to explore the concentration distribution of Ca. In this manuscript, a novel TP fluorescence calcium probe (BAPTAVP) with positive charges, based on the classical Ca indicator of BAPTA (1,2-bis(2-aminophenoxy)-ethane--tetra acetic acid), and a conjugated polymer (PCBMB) with negative charges were designed and synthesized.