Heterodimers of Aromadendrane Sesquiterpenoid with Benzoquinone from the Chinese Liverwort .

Mylnudones A-G (-), unprecedented 1,10--aromadendrane-benzoquinone-type heterodimers, and a highly rearranged aromadendrane-type sesquiterpenoid (), along with four known analogs (-), were isolated from the liverwort . Compounds - and , bearing tricyclo[6.2.

Combining NMR-Based Metabolic Profiling and Genome Mining for the Accelerated Discovery of Archangiumide, an Allenic Macrolide from the Myxobacterium SDU8.

An unprecedented 19-membered allenic macrolide archangiumide () was discovered from the myxobacterium SDU8 by integrating NMR-based metabolic profiling and genome mining. Its biosynthesis pathway was proposed based on the architectural analysis of the encoding -AT PKS genes and validated by isotope labeling. The methodology of combing 2D NMR-based metabolic profiling and bioinformatics-aided structure prediction, as exemplified by this study, is anticipated to improve discovery efficiency of a broader range of microbial "dark matter".

Two New Quinazoline Derivatives from the Moss Endophytic Fungus Aspergillus sp. and Their Anti-inflammatory Activity.

Two new quinazoline derivatives versicomides E (1) and F (2), and 10 known compounds (3-12) were isolated from the moss endophytic fungus Aspergillus sp. Their structures were determined on the basis of extensive spectroscopic data analysis and ECD calculations. Among them, the compound 7 (6-hydroxy-3-methoxyviridicatin) was first reported as a natural product.

Asperunguisins A-F, Cytotoxic Asperane Sesterterpenoids from the Endolichenic Fungus Aspergillus unguis.

Six new asperane-type sesterterpenoids, asperunguisins A-F (1-6), were isolated from the endolichenic fungus Aspergillus unguis, together with a known analogue, aspergilloxide (7); these are rare asperane-type sesterterpenoids, characterized by a unique hydroxylated 7/6/6/5 tetracyclic system. The structures of asperunguisins A-F (1-6) were elucidated on the basis of spectroscopic methods (NMR and HRESIMS), X-ray single-crystal diffraction analysis, ECD calculations, and biogenetic considerations. Asperunguisin C (3) showed cytotoxicity against the human cancer cell line A549 with an IC value of 6.

Secondary metabolites from the endolichenic fungus .

The isolation of the cytotoxic fractions from the endolichenic fungus yielded six new metabolites, including five polyketides (ophiofuranones A (1) and B (2), with unusual furopyran-3,4-dione-fused heterocyclic skeletons, ophiochromanone (3), ophiolactone (4), and ophioisocoumarin (5)), one sesquiterpenoid ophiokorrin (10), and nine known compounds. Their structures were established on the basis of the analysis of HRESIMS and NMR spectroscopic data. ECD calculations, GIAO NMR shift calculations and single-crystal X-ray diffraction were employed for the stereo-structure determination.

Diterpenoids from the Chinese liverwort Frullania hamatiloba and their Nrf2 inducing activities.

Six previously undescribed labdane diterpenoids, frullanians A-F, along with five known diterpenoids, were isolated from the Chinese liverwort Frullania hamatiloba Stephani. Their structures were determined using NMR data, electronic circular dichroism (ECD) calculations as well as the single crystal X-ray diffraction measurement. NAD(P)H: QR (quinone reductase) assay demonstrated that frullanian D and four known compounds displayed antioxidant effect mediated via Nrf2 (Nuclear factor-erythroid 2-related factor 2) induction.

Cytotoxic p-Terphenyls from the Endolichenic Fungus Floricola striata.

Eleven new p-terphenyls, floricolins K-U (1-11), together with 13 biosynthetically related known compounds (12-24) were isolated from an endolichenic fungus, Floricola striata. Their structures were elucidated by extensive spectroscopic analyses and single-crystal X-ray diffraction measurements. The newly isolated p-terphenyls inhibited the growth of A2780, MCF-7, and A549 cell lines.

Two new α-pyrone derivatives from an endolichenic fungus Tolypocladium sp.

Two new α-pyrone derivatives, tolypocladones A (1) and B (2), together with five known compounds were isolated from an endolichenic fungus Tolypocladium sp. (4259a). The structures of all the compounds were determined by analysis of their MS and NMR data.

p-Terphenyl Derivatives from the Endolichenic Fungus Floricola striata.

Ten new p-terphenyl derivatives, floricolins A-J (1-10), together with six known compounds (11-16), were isolated from the extract of the endolichenic fungus Floricola striata. Chemical structures of these compounds were elucidated using spectroscopic data (HRESIMS and NMR). Among them, 9 and 10 were enantiomeric mixtures, and their configurations were established by single-crystal X-ray diffraction analysis using Cu Kα radiation.

[Chemical Constituents from Rhizome of Valeriana jatamansi].

Objective: To study the chemical constituents from the rhizome of Valeriana jatamansi.

Methods: The chemical constituents were separated and purified by silica gel, medium pressure column chromatography, and preparative HPLC. Their structures were determined by physicochemical properties and spectral data.

A new diketopiperazine heterodimer from an endophytic fungus Aspergillus niger.

One new diketopiperazine heterodimer, asperazine A (1), and eight known compounds, asperazine (2), cyclo(d-Phe-l-Trp) (3), cyclo(l-Trp-l-Trp) (4), 4-(hydroxymethyl)-5,6-dihydro-pyran-2-one (5), walterolactone A (6), and campyrones A-C (7-9), were isolated from an endophytic fungus Aspergillus niger. Their structures were determined unequivocally on the basis of extensive spectroscopic data analysis. This is the first report of the presence of compound 3 as a natural product.

[Epidemiological survey of congenital heart disease among people aged from 4 to 18 in Haidong area of Qinghai province].

Objective: To investigate the epidemiological characteristics of congenital heart disease (COHD) among 4 to 17 years old children in Haidong area of Qinghai province.

Methods: All 97 718 children were surveyed with the following 3 steps: prescreening, countershock and confirmation with color Doppler. The distribution patterns were analyzed by national groups, ages and genders respectively.