C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases.

Unlabelled: Loss-of-function mutants are fundamental resources for gene function studies. However, it is difficult to generate viable and heritable knockout mutants for essential genes. Here, we show that targeted editing of the C-terminal sequence of the embryo lethal gene () results in weak mutants.

Immunochemotherapy for primary central nervous system lymphoma with rituximab, methotrexate, cytarabine and dexamethasone: Retrospective analysis of 18 cases.

The incidence of primary central nervous system lymphoma (PCNSL) has increased in the last two decades and the clinical research regarding the treatment for PCNSL patients has also increased. However, the optimal induction chemotherapy has not been fully established. In the present retrospective study, the aim was to analyze the outcome in PCNSL patients treated with the combination of rituximab, methotrexate (MTX), cytarabine (Ara-C) and dexamethasone (R-MAD).

[A preliminary study of prognostic value of thrombocytopenia in patients with primary myelodysplastic syndromes].

Objective: To investigate the prognostic value of thrombocytopenia in patients with primary myelodysplastic syndromes (MDS).

Methods: Four hundred and nineteen primary MDS patients were retrospectively analyzed. Kaplan-Meier method, Log-rank test and COX regression model were used to evaluate factors that influence the prognosis.

[Clinical features and survival analysis in primary myelodysplastic syndromes patients with immunological abnormalities].

Objective: To analyze the clinical features and survival time in primary myelodysplastic syndromes (MDS) patients accompanied with immunological abnormalities.

Methods: The clinical information, laboratory findings and survival time in 194 untreated primary MDS patients with complete immunological laboratory tests or a past history of autoimmune disease were analyzed retrospectively.

Results: There were 37/194 cases (19.