Sialyllactose is an acidic oligosaccharide that has an immune-protective effect against pathogens and contributes to developing the immune system and intestinal microbes. In this study, a method for the determination of 3'-sialyllactose by high-performance liquid chromatography tandem mass spectrometry was established. The sample was treated with 0.
View Article and Find Full Text PDFA newly discovered natural hybrid, Iris×ampliflora Y.E. Xiao, F.
View Article and Find Full Text PDFIn order to elucidate the influence of T lymphocytes subpopulations on B lymphocytes immune response, in this paper, CD4-1, CD4-2, CD8 T lymphocytes and B lymphocytes responses to thymus-independent (TI) or thymus-dependent (TD) antigen plus immunosuppressant were investigated in flounder (Paralichthys olivaceus). The results showed that in LPS-immunized group, the percentages of CD4-1, CD4-2, CD8β T (PCD4-1 T, PCD4-2 T and PCD8β T) lymphocytes in peripheral blood leucocytes (PBLs) had no significant variations, the percentages of IgM B (PIgM B) lymphocytes and LPS-specific antibodies (LA) significantly increased and peaked at 3 or 4 week post-injection; CsA had no inhibition on both T/B lymphocytes and LA; RaPa only suppressed the PIgM B lymphocytes and LA, and the inhibition maximum (Imax) were about 35% and 20%, respectively. In KLH-immunized group, the PCD4-1, PCD4-2 and PCD8β T lymphocytes significantly increased and peaked at 3 or 5 day, successively the PIgM B lymphocytes and KLH-specific antibodies (KA) significantly increased to the peak at 5 week; the PCD4-1, PCD4-2 T and PIgM B lymphocytes and LA were inhibited significantly by both CsA and RaPa, and the Imax on them were 13%-33%, 11%-25%, 19%-34%, 22%-26%, respectively, while the PCD8β T lymphocytes showed no significant suppression.
View Article and Find Full Text PDFPremise Of The Study: Declining orchid populations have made it necessary to prioritize the study of population structure and genetic diversity for species including (Orchidaceae).
Methods And Results: A biotin-streptavidin capture method was used to construct a microsatellite library for . A total of 15 polymorphic nuclear microsatellite loci were isolated and characterized using 47 individuals from two natural populations in China.
In acquired immunity, T lymphocytes regulate the immune responses of B lymphocytes, including the IgM B lymphocyte counts and antibody production. In this paper, Cyclosporine A (CsA) and Rapamycin (RaPa) were used, and their inhibition on T lymphocytes and immune responses of B lymphocytes in flounder (Paralichthys olivaceus) were investigated. Flounder was injected with Keyhole Limpet Hemocyanin (KLH), a mixture of KLH and CsA (KLH + CsA), or a mixture of KLH and RaPa (KLH + RaPa).
View Article and Find Full Text PDFPremise Of The Study: Microsatellite primers were developed in Iris ensata (Iridaceae) to provide polymorphic markers for further studies into population genetics. •
Methods And Results: Thirteen polymorphic microsatellite loci were isolated from I. ensata.
The study with in-door experiment showed that the germination and seedling rates of Potamogeton crispus were increased under light but without matrix. Matrix promoted root growth, but light could not. The internode's length was obviously longer under dark than under light.
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