scVGATAE: A Variational Graph Attentional Autoencoder Model for Clustering Single-Cell RNA-seq Data.

Single-cell RNA sequencing (scRNA-seq) is now a successful technology for identifying cell heterogeneity, revealing new cell subpopulations, and predicting developmental trajectories. A crucial component in scRNA-seq is the precise identification of cell subsets. Although many unsupervised clustering methods have been developed for clustering cell subpopulations, the performance of these methods is prone to be affected by dropout, high dimensionality, and technical noise.

Single image deraining via wide rectangular regional blocks and dual attention complementary enhancement network.

Rain is a common weather phenomenon, and the challenge of removing rain streaks from a single image is crucial due to its detrimental impact on image quality and the extraction of valuable background information. Existing methods commonly rely on specific assumptions regarding rain models, which restricts their ability to accommodate a wide range of real-world scenarios. To overcome this limitation, these methods often require complex optimization techniques or stepwise refinement strategies.

A Novel Method for Multi-subject fMRI Data Analysis: Independent Component Analysis with Clustering Embedded (ICA-CE).

The analysis of multi-subject functional magnetic resonance imaging (fMRI) data and the extraction of accurate brain functional networks (FNs) are of great importance. However, traditional independent component analysis (ICA) methods perform analysis on multi-subject fMRI data under the condition of known or assumed classes of subjects, which may decrease its ability to extract accurate individual brain FNs. Although a previous method named clusterwise ICA (C-ICA) clusters subjects and obtains shared FNs in group-level for each class, its clustering performance on complex data is not ideal.

Fate of ofloxacin in rural wastewater treatment facility: Removal performance, pathways and microbial characteristics.

Ofloxacin (OFL) with high biological activity and antimicrobial degradation is a kind of the typical high concentration and environmental risk antibiotics in rural sewage. In this paper, a combined rural sewage treatment facility based on anaerobic baffled reactor and integrated constructed wetlands was built and the removal performance, pathway and mechanism for OFL and conventional pollutants were evaluated. Results showed that the OFL and TN removal efficiency achieved 91.

Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus.

Avian hepatitis E virus (HEV) is the main etiologic pathogen of chicken big liver and spleen disease which is widely prevalent in China in recent years. However, due to the lack of a highly effective culture system in vitro, a genetically engineered subunit vaccine is the main direction of vaccine development. In this study, ORF3 genes of VaHEV strain from laying hens and YT-aHEV strain from broilers were amplified, respectively, and ORF3 protein was successfully expressed by prokaryotic expression system.

Fate of antibiotic resistance genes in cultivation substrate and its association with bacterial communities throughout commercial production of Agaricus bisporus.

Animal manure is an important raw material for Agaricus bisporus production; however, it is also a reservoir for antibiotic residues, antibiotic resistance genes (ARGs), and antibiotic-resistant bacteria. Little is known about the influence of the commercial cultivation of A. bisporus on the dynamics of ARGs and the underlying mechanisms that cause their variations.

Fast magnesiation kinetics in α-AgS nanostructures enabled by an in situ generated silver matrix.

Here we first present a novel cathode material of α-Ag2S for rechargeable Mg batteries. Due to the unique cation displacement reaction mechanism and the in situ generated uniformly distributed silver matrix, α-Ag2S nanostructures deliver remarkably improved magnesiation kinetics compared to conventional transition metal sulfides.

Detection of methoxylated and hydroxylated polychlorinated biphenyls in sewage sludge in China with evidence for their microbial transformation.

The concentrations of methoxylated polychlorinated biphenyls (MeO-PCBs) and hydroxylated polychlorinated biphenyls (OH-PCBs) were measured in the sewage sludge samples collected from twelve wastewater treatment plants in China. Two MeO-PCB congeners, including 3'-MeO-CB-65 and 4'-MeO-CB-101, were detected in three sludge with mean concentrations of 0.58 and 0.