sp. nov., a novel bacterium isolated from the culture of a dinoflagellate .

A Gram-negative, rod-shaped and light pink-pigmented bacterial strain, designated Amp-Y-6, was isolated from the culture of a dinoflagellate CCMP1314. It can produce bacteriochlorophyll . The 16S rRNA gene of strain Amp-Y-6 had the highest sequence similarity with CCMM001 of 98.

A Unique Combination of Mn and Aluminum Adjuvant Acted the Synergistic Effect.

Introduction: The development of combinatorial adjuvants is a promising strategy to boost vaccination efficiency. Accumulating evidence indicates that manganese exerts strong immunocompetence and will become an enormous potential adjuvant. Here, we described a novel combination of Mn plus aluminum hydroxide (AH) adjuvant that significantly exhibited the synergistic immune effect.

The cooperation between orf virus and leads to intractable lesions in skin infection.

A large amount of evidence shows that different kinds of microorganisms can jointly cope with environmental pressures including cell hosts. For example, in many cases, it has been found that secondary or mixed infection of animals caused by ORFV (an epitheliophilic Parapoxvirus) and bacteria (such as Staphylococcus aureus or Streptococcus) shows a mutual aid mode that indirectly leads to the deterioration of the disease. However, the lack of research on the co-pathogenic mechanism, including how to hijack and destroy the cell host in the pathological microenvironment, has hindered the in-depth understanding of the pathogenic process and consequences of this complex infection and the development of clinical treatment methods.

Transient breathing dynamics during extinction of dissipative solitons in mode-locked fiber lasers.

The utilization of the dispersive Fourier transformation approach has enabled comprehensive observation of the birth process of dissipative solitons in fiber lasers. However, there is still a dearth of deep understanding regarding the extinction process of dissipative solitons. In this study, we have utilized a combination of experimental and numerical techniques to thoroughly examine the breathing dynamics of dissipative solitons during the extinction process in an Er-doped mode-locked fiber laser.

PEI-PLGA nanoparticles significantly enhanced the immunogenicity of IsdB proteins from Staphylococcus aureus.

Introduction: Staphylococcus aureus seriously threatens human and animal health. IsdB of the iron surface determinant B protein (IsdB) from S. aureus exhibits the strong immunogenicity, but its immunoprotective effect is still to be further promoted.

Low-loss skimming waveguides with controllable mode leakage for on-chip saturable absorbers.

Emerging 3D photonic circuits would greatly benefit from the ability to integrate skimming waveguides with low loss and controllable inscription depth into photonic circuits. These waveguides allow for the interaction of guiding light directly with external modulation signals and enable programmable photonic circuits. Here, we report the fabrication of a novel photonic-lattice-like skimming waveguide (PLLSW) using femtosecond laser writing.

Dichromatic "Breather Molecules" in a Mode-Locked Fiber Laser.

Bound states of solitons ("molecules") occur in various settings, playing an important role in the operation of fiber lasers, optical emulation, encoding, and communications. Soliton interactions are generally related to breathing dynamics in nonlinear dissipative systems, and maintain potential applications in spectroscopy. In the present work, dichromatic breather molecules (DBMs) are created in a synchronized mode-locked fiber laser.

Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice.

Background: Staphylococcus aureus is an opportunistic pathogen responsible for various infections with diverse clinical presentation and severity. The α-hemolysin is a major virulence factor in the pathogenesis of S. aureus infections.

ORFV can carry TRAP gene expression via intracellular CRISPR/Cas9 gene editing technology.

Orf is an acute and highly contracted human and animal infection caused by orf virus (ORFV), which mainly affects sheep, goats, and other species. Clinically, opportunistic or conditional pathogens such as Staphylococcus aureus (S. aureus) are often detected in cases of orf, which greatly increases the risk of disease progression and clinical death.

Peroxiredoxin 5 protects HepG2 cells from ethyl β-carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways.

Peroxiredoxin 5 (PRDX5) is the member of Prxs family, widely reported to be involved in various types of cell death. We previously found that knockdown increases the susceptibility of cell death upon oxidative stress treatment. Ethyl β-carboline-3-carboxylate (β-CCE), an alkaloid extracted from , has been reported to play a role in neuronal disease, but its anti-cancer potential on liver cancers remains unknown.

Ethyl β-Carboline-3-Carboxylate Increases Cervical Cancer Cell Apoptosis Through ROS-p38 MAPK Signaling Pathway.

Background/aim: Ethyl β-carboline-3-carboxylate (β-CCE) is one of the effective ingredients of Picrasma quassioides (P. quassioides). As a β-carboline alkaloid, it can antagonize the pharmacological effects of benzodiazepines by regulating neurotransmitter secretion through receptors, thus affecting anxiety and physiology.

The OH system: A panorama view of the PPV-host interaction.

Poxviruses are a family of specialized cytoplasm-parasitic DNA viruses that replicate and assembly in virus factory. In Parapoxvirus (PPV) genus, with the orf virus (ORFV) as a representative species of this genus, their behaviors are significantly different from that of Orthopoxvirus, and the plots of viral practical solutions for evading host immunity are intricate and fascinating, particularly to anti-host and host's antiviral mechanisms. In order to protect the virus factory from immune elimination caused by infection, PPVs attempt to interfere with multiple stress levels of host, mainly by modulating innate immunity response (IIR) and adaptive immunity response (AIR).

Als3-Th-cell-epitopes plus the novel combined adjuvants of CpG, MDP, and FIA synergistically enhanced the immune response of recombinant TRAP derived from Staphylococcus aureus in mice.

Introduction: Staphylococcus aureus (S. aureus) is a gram-positive opportunistic pathogen, there are currently no high effective vaccine against S. aureus in humans and animals, the development of an efficient vaccine remains an important challenge to prevent S.

IL-17A Secreted by Th17 Cells Is Essential for the Host against Infections.

is an important bacterial pathogen and causative agent of diseases including neonatal sepsis and meningitis, as well as infections in healthy adults and pregnant women. Although antibiotic treatments effectively relieve symptoms, the emergence and transmission of multidrug-resistant strains indicate the need for an effective immunotherapy. Effector T helper (Th) 17 cells are a relatively newly discovered subpopulation of helper CD4 T lymphocytes, and which, by expressing interleukin (IL)-17A, play crucial roles in host defenses against a variety of pathogens, including bacteria and viruses.

Immunogenicity of multi-epitope vaccines composed of epitopes from Streptococcus dysgalactiae GapC.

Glyceraldehyde-3-phosphate dehydrogenase C (GapC) of Streptococcus dysgalactiae (S. dysgalactiae) is a highly conserved surface protein that can induce a protective immune response against S. dysgalactiae infection.

Full-field real-time characterization of creeping solitons dynamics in a mode-locked fiber laser.

Creeping solitons, which belong to the class of pulsating solitons, can be meaningful for fundamental physics owing to their fruitful nonlinear dynamics. Their characteristics in mode-locked lasers have been studied theoretically, but it is difficult to experimentally observe evolution dynamics in real time. Here, we have experimentally observed the temporal and spectral evolution dynamics of creeping solitons in a passively mode-locked fiber laser by employing time-lens and dispersive Fourier transform technique.

Laboratory Diagnosis of a NZ7-like Orf Virus Infection and Pathogen Genetic Characterization, Particularly in the Gene.

Orf is a widespread contagious epithelial viral disease found particularly in most sheep breeding countries in the world. Recently, an orf virus (ORFV) strain OV-HLJ05 was isolated from an outbreak in northeast China. Three genes of interest including ORFV011 (B2L), ORFV059 (F1L), and ORFV132 (VEGF) of ORFV, were recruited to identify and genetically characterize this newly isolated virus.

Unveiling external motion dynamics of solitons in passively mode-locked fiber lasers.

Real-time measurement of ultrafast pulses together with high temporal resolution and long recording length is an urgent requirement of all optical communication systems and nonlinear science. Here, external motion dynamics of soliton pairs in mode-locking ultrafast fiber lasers can be single-shot characterized with long recording length, by using an asynchronous four-wave-mixing (FWM)-based temporal magnifier (AFTM) system. Recording length of more than one thousand roundtrips can be achieved through the AFTM system.

Microfiber-assisted gigahertz harmonic mode-locking in ultrafast fiber laser.

Optoacoustic interaction can be strongly enhanced in tiny core fibers, and it holds significant potential for stable harmonic mode-locking at gigahertz (GHz) and higher repetition rate. In this Letter, we propose and demonstrate a microfiber-assisted GHz harmonic mode-locking fiber laser, which is achieved by the enhanced optomechanical coupling between cavity modes in microfiber with the waist length of ∼16 and the waist diameter of ∼1.56µ.

Peroxiredoxin I deficiency increases pancreatic β‑cell apoptosis after streptozotocin stimulation via the AKT/GSK3β signaling pathway.

Apoptosis of pancreatic β‑cells is involved in the pathogenesis of type I and II diabetes. Peroxiredoxin I (Prx I) serves an important role in regulating cellular apoptosis; however, the role of Prx I in pancreatic β‑cell apoptosis is not completely understood. In the present study, the role of peroxiredoxin 1 (Prx I) during streptozotocin (STZ)‑induced apoptosis of pancreatic β‑cells was investigated.

Anti-tumor Properties of Extracts in H-Ras Liver Cancer Are Mediated Through ROS-dependent Mitochondrial Dysfunction.

Background: Picrasma quassioides (PQ) is a traditional Asian herbal medicine with anti-tumor properties that can inhibit the viability of HepG2 liver cancer cells. H-Ras is often mutated in liver cancer, however, the effect of PQ treatment on H-Ras mutated liver cancer is unclear. This study aimed to investigate the role of PQ on ROS accumulation and mitochondrial dysfunction in H-ras mutated HepG2 (HepG2) cells.

Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway.

Background/aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P.

The novel combinations of CTB, CpG, and aluminum hydroxide significantly enhanced the immunogenicity of clumping factor A of .

Unlabelled: Here, we prepared the novel combined adjuvants, CTB as intra-molecular adjuvant, CpG and aluminum hydroxide (Alum) to strengthen the immunogenicity of clumping factor A of (). The protein-immunoactive results showed CTB-ClfA elicited the strong immune responses to serum from mice immunized with CTB and ClfA, respectively. The mice immunized with CTB-ClfA plus CpG and Alum adjuvant exhibited significantly stronger CD4 T cell responses for IFN-γ, IL-2, IL-4, and IL-17 and displayed the higher proliferation response of splenic lymphocytes than the control groups, in addition, these mice generated the strongest humoral immune response against ClfA among all groups.

A Putative Efflux Transporter of the ABC Family, YbhFSR, in Functions in Tetracycline Efflux and Na(Li)/H Transport.

ATP-binding cassette transporters are ubiquitous in almost all organisms. The genome is predicted to encode 69 ABC transporters. Eleven of the ABC transporters are presumed to be exporters, of which seven are possible drug export transporters.