CRISPR/Cas9-Mediated Disruption of the Gene and Its Impact on Growth, Development, and Penicillin Production in .

is a filamentous fungus of great biotechnological importance due to its role as an industrial producer of the antibiotic penicillin. However, despite its significance, our understanding of the regulatory mechanisms governing biological processes in this fungus is still limited. In fungi, zinc finger proteins containing a Zn(II)Cys domain are particularly interesting regulators.

Pr Affects the Production of Roquefortine C, Mycophenolic Acid, and Andrastin A in , but It Has Little Impact on Asexual Development.

The regulation of fungal specialized metabolism is a complex process involving various regulators. Among these regulators, LaeA, a methyltransferase protein originally discovered in spp., plays a crucial role.