Evaluation of Female Recipient Infertility and Donor Spermatogonial Purification for Germ Cell Transplantation in .

Since the advent of germ cell transplantation (GCT), it has been widely used in shortening the fish breeding cycle, sex-controlled breeding and the protection of rare and endangered fish. In this study, the effectiveness of female sterile recipient preparation and donor stem cell isolation and purification were comprehensively evaluated for spermatogonial stem cell transplantation (SSCT) in . The best way to prepare sterile recipients was found to be giving three-year-old fish four intraovarian injections of busulfan (20 mg/kg body weight) combined with exposure to a high temperature (28 °C) after the spawning season compared with the two other ways, which induced apoptosis of most of the endogenous germ cells, resulting in shrinkage of the spawning plate and enlargement of the ovarian lumen.

Characterization and function of Japanese flounder (Paralichthys olivaceus) slc2a6 in response to lymphocystis disease virus infection.

Slc2a6 is a member of the slc2 family (solute carrier 2 family) and previous reports have indicated its involvement in the inflammatory response. Slc2a6 is regulated by the NF-ĸB signaling pathway. This study investigated the differential expression of slc2a6 in the early embryonic development of Japanese flounder, revealing that the early gastrula stage had the highest level of slc2a6 expression.

A New Cell Line Derived from the Spleen of the Japanese Flounder () and Its Application in Viral Study.

A new cell line Japanese flounder spleen (JFSP) derived from the spleen of Japanese flounder () was established and characterized in this study. The JFSP cells grew rapidly at 29 °C, and the optimum fetal bovine serum concentration in the L-15 medium was 15%. Cells were subcultured for more than 80 passages.

Enhanced chiral sensing in achiral nanostructures with linearly polarized light.

Chiral plasmonic nanostructures can generate large superchiral near fields owing to their intrinsic chirality, leveraging applications for molecule chirality sensing. However, the large structural chirality of chiral nanostructures poses the risk of overshadowing molecular chiral signals, hampering the practical application of chiral nanostructures. Herein, we propose an achiral nanorod that shows no structural chirality and presents strong superchiral near-fields with linearly polarized incidence.

Nanophotonic devices based on magneto-optical materials: recent developments and applications.

Interaction between light and magnetism in magneto-optical (MO) nanophotonic devices has been actively studied in the past few years. The recent development of MO all-dielectric resonators and metasurfaces has led to the emergence of various novel MO phenomena that were not observed in their bulk counterparts. For example, a large s-polarized transverse MO Kerr effect can be observed at magnetic resonance wavelength, which cannot exist in the bare MO films.

Applying copulas to predict the multivariate reduction effect of best management practices.

Best management practices (BMPs) have been widely applied to mitigate non-point source (NPS) pollution in agricultural watersheds. However, a prediction of the multivariate reduction effect of NPS pollutants by BMPs considering its stochastic nature has not been conducted. A new modeling approach combining a hydrological model and copulas was proposed to predict the multivariate effect of BMPs fully considering the stochastic characteristics of BMPs effects and the dependence structure between them.

Down-regulation of lncRNA NEAT1 regulated by miR-194-5p/DNMT3A facilitates acute myeloid leukemia.

Objective: miR-194-5p and NEAT1 have been reported to be associated with multiple malignancies, but their roles in acute myeloid leukemia (AML) remains not fully understood.

Methods: Bone marrow samples were collected for monocyte separation. qRT-PCR assay was performed to investigate the expression patterns of NEAT1 and miR-194-5p in AML.

Source identification and impact of landscape pattern on riverine nitrogen pollution in a typical urbanized watershed, Beijing, China.

This study explored the sources of nitrate and the impact of landscape pattern on nitrogen pollution in the highly urbanized Beiyun River Watershed, China during 2016 by applying a dual stable isotope approach (δN-NOand δO-NO) combined with multiple statistical analyses. The sources of riverine nitrate principally originated from manure and sewage, nitrification of soil nitrogen, fertilizer nitrification, and atmospheric deposition. A Bayesian model was used to estimate the source contributions and results showed that manure and sewage were the major contributors to river nitrate with combined proportions of 77.

Natural Killer Group 2A Expressed on Both Peripheral CD3CD56NK Cells and CD3CD8T Cells Plays a Pivotal Negative Regulatory Role in the Progression of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.

To explore the role of surface receptors natural killer group 2A (NKG2A) and natural killer group 2D (NKG2D) on CD3CD8T cells and CD3CD56NK cells in the progression of hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF), we measured the expression of NKG2A and NKG2D on the surface of these 2 types of circulating cells by flow cytometry in 3 groups. One group consists of 36 patients with chronic hepatitis B (CHB), another one consists of 22 patients with HBV-related ACLF, and the last one has 12 normal controls (NC). The experimental result indicated that there was no significant difference in the proportion of CD3CD8T cells in total lymphocytes between the 3 groups.

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System.

To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.

Direct competitive chemiluminescence immunoassays based on gold-coated magnetic particles for detection of chloramphenicol.

Direct competitive chemiluminescence immunoassays (CLIA) based on gold-coated magnetic nanospheres (Au-MNPs) were developed for rapid analysis of chloramphenicol (CAP). The Au-MNPs were modified with carboxyl groups and amino groups by 11-mercaptoundecanoic acid (MUA) and cysteamine respectively, and then were respectively conjugated with CAP base and CAP succinate via an activating reaction using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). NSP-DMAE-NHS, a new and effective luminescence reagent, was employed to label anti-CAP antibody (mAb) as a tracer in direct CLIA for CAP detection using a 'homemade' luminescent measurement system that was set up with a photomultiplier tube (PMT) and a photon counting unit linked to a computer.

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes.

A method of immobilizing clenbuterol (CLEN) on the sensor chip for spectral surface plasmon resonance imaging (SPRi) was experimentally investigated. The bioprobes on the sensor chip were prepared by immobilizing bovine serum albumin (BSA) protein and conjugating CLEN molecules to BSA, which provides more active points and free orientations for specific binding. The calibration curve showed that the wavelength resonance shift decreased as the concentration of CLEN analyte increased, consistent with the inhibition principle.

Serum IL-21 levels associated with chronic hepatitis B and hepatitis B-related liver failure.

The aim of the present study was to investigate the role of interleukin (IL)-21 in chronic hepatitis B virus (HBV) infection. IL-21 stimulates T and B cell responses and plays a role in the control of chronic viral infections. Serum IL-21 levels were measured by enzyme immunoassay in 109 patients with chronic HBV infection at various clinical stages, as well as in 19 healthy controls (HCs).

Elevated serum IL-21 levels in hantavirus-infected patients correlate with the severity of the disease.

Hemorrhagic fever with renal syndrome (HFRS) is an acute viral infection caused by Hantavirus (HTV). Capillary leakage is one of the hallmarks of HTV infection. The mechanisms underlying the pathogenesis of HFRS are not completely understood.

Restoring the Treg cell to Th17 cell ratio may alleviate HBV-related acute-on-chronic liver failure.

Aim: To investigate the role of T helper 17 cells (Th17) and regulatory T cells (Treg) in hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF).

Methods: We enrolled 79 patients with HBV infection into the study, 50 patients with HBV-related ACLF and 29 patients with chronic hepatitis B (CHB), from the First Affiliated Hospital of Medical College from January 2009 to June 2012. The ACLF patients were diagnosed according to the criteria recommended by The 19(th) Conference of the Asian Pacific Association for the Study of the Liver in 2009.

Detection of HLA-B*27 gene using a spectral plasmon resonance imaging system.

Detection of HLA-B*27 gene is clinically important due to its strong association with diseases, such as ankylosing spondylitis. Nucleic acid-based biosensors represent a promising clinical tool for gene diagnosis. Surface plasmon resonance (SPR) can be used to monitor DNA-DNA hybridization in real-time and without any prior labeling step.

Identification of cyclophilin A as a potential prognostic factor for clear-cell renal cell carcinoma by comparative proteomic analysis.

The high recurrence and improved survival rate of clear-cell renal cell carcinoma (ccRCC) demand for continuous effort to search for novel prognostic factors. Herein a comparative proteomics approach based on two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify differentially expressed proteins between ccRCC cell line RLC-310 and normal renal cell line HK-2. Of the 31 proteins identified, Cyclophilin A (CypA) was a newly identified differentially expressed protein in ccRCC cell line.

Characterization of genes associated with different phenotypes of human bladder cancer cells.

To identify genes associated with morphological phenotypes of human bladder transitional cell carcinoma, we used suppression subtractive hybridization (SSH) to create a subtractive cDNA library of two established cell lines, BLZ-211 and BLS-211, derived from a patient with transitional cell carcinoma of the bladder, then to screen for differentially expressed genes. Real-time reverse transcription-polymerase chain reaction was used to further confirm the selected differentially expressed genes. Forward and reverse subtractive cDNA libraries yielded 168 and 305 putative clones, and among them more than 90% contained the inserts.

[Differential expression of immune-associated genes in two subcloned cell lines from a same human bladder cancer].

Aim: To screen and identify differentially expressed genes in subcloned lines from a same human bladder transitional cell carcinoma (TCC).

Methods: Two bladder TCC cell lines (BLX and BLS-211) with different phenotypes but same origin were used to screen for differentially expressed genes by suppression subtractive hybridization (SSH).

Results: 9 over-expressed genes in BLX and 15 in BLS-211 cells were obtained, respectively.

[Screening and identification of heterogeneous phenotype-associated genes in human bladder cancer].

Objective: To screen and identify heterogeneous phenotype-associated genes of human bladder transitional cell carcinoma.

Methods: Subtractive cDNA libraries was established by means of suppression subtractive hybridization (SSH) on the basis of two human bladder transitional cell carcinoma cell lines (BLZ-211 and BLS-211) derived from the same patient, which had similar changes in chromosomes but different cell phenotypes (in terms of cell shape, susceptibility to 5-Fu and tumorigenic capacity). The positive clones in the library were selected for screening differentially expressed gene fragments by sequence analysis and blasting, and Northern blotting was performed to confirm the differentially expressed genes.

[Liposome-mediated human CD40 gene transfection and human umbilical vein endothelial ECV-304 cells].

Objective: To construct an eukaryotic expression vector containing human CD40 gene for its efficient, continuous and stable expression in human umbilical vein endothelial ECV-304 cells.

Methods: The recombinant plasmid pUCD40 was digested with endonucleases to obtain human CD40 gene fragment, which was cloned into pCDNA3.1 vector to construct recombinant eukaryotic expression vector pCDNA3.

[Establishment and characterization of a new squamous cell carcinoma cell line CS1213 from the human uterine cervix].

Objective: To establish a human cervical carcinoma cell line.

Methods: A primary culture was initiated from malignant tissue collected by dissection of cervical biopsy specimens. Characterizing cells in culture which included morphological observation, biological and karyotypic analysis, experimental tumorigenesis and the expression of p53, bcl-2 and Ki67 genes was carried out.

[Study on expression of cell cycle-related genes in subclonal cell lines of human cervical carcinoma].

Objective: To explore the role of expression of cell cycle-related genes in cervical carcinoma cell lines.

Methods: A series of expression microarray analysis of two homologous cervical carcinoma subclonal cell lines were initiated by cDNA microarray which represent a set of 234 human cell cycle-related genes.

Results: In normal medium, the percent of G(1) phase in CS03 cells was higher than in CS07 cells dramatically, but the percent of S phase in CS07 cells was more than in CS03 cells.