Publications by authors named "Yuchuan Zhao"
Background: microRNAs (miRNAs) were recognized as a promising source of diagnostic biomarker. Herein, we aim to evaluate the performance of an ultrasensitive method for detecting serum miRNAs using single molecule arrays (Simoa).
Methods: In this study, candidate miRNAs were trained and tested by RT-qPCR in a cohort of PTB patients.
A Profile of Drug-Resistant Mutations in Isolates from Guangdong Province, China.
Indian J Microbiol
September 2024
Article Synopsis
- - The study focuses on tuberculosis (TB) strains in Guangdong Province, China, examining the distribution, transmission, and drug resistance of Mycobacterium tuberculosis (Mtb) among 20,662 patients from 31 surveillance sites between 2016 and 2018.
- - Researchers conducted minimum inhibitory concentration (MIC) testing on 14 anti-TB drugs and performed whole-genome sequencing on 713 clinical Mtb isolates to analyze drug resistance patterns and genetic mutations.
- - Findings revealed the lineage distribution and drug-resistant gene profiles of Mtb strains, identifying 16 novel mutation loci linked to resistance to multiple drugs, providing valuable insights for TB prevention and control strategies in the region.
Article Synopsis
- Rifampicin (RIF) and multidrug-resistant tuberculosis (TB) pose significant public health challenges, and quicker diagnostic methods are needed compared to traditional testing that takes weeks.
- MeltPro TB and whole-genome sequencing (WGS) were evaluated for their effectiveness in detecting drug resistance to RIF, isoniazid (INH), and other medications in TB patients from Guangdong province.
- MeltPro TB demonstrated similar detection rates to WGS for RIF, INH, and streptomycin resistance, but its effectiveness for ethambutol (EMB) and fluoroquinolone (FQ) resistance was limited, indicating the need for further optimization before wider use.
In reinforced concrete (RC) structures, new-to-old concrete interfaces are widely present due to precast splices, repairs, and construction joints. In this paper, both monolithic and segmental specimens were fabricated with five kinds of water-cement ratios, including ordinary and high-strength concrete. The impressed current-accelerated corrosion test was used, and the degree of reinforcement corrosion was controlled by Faraday's Law.
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- * This study utilized laboratory evolution to identify mutational pathways leading to amikacin resistance, finding that mutations affected susceptibility to other antibiotics such as isoniazid and levofloxacin.
- * Whole-genome sequencing revealed that the A1401G mutation predominates in resistant Mtb strains, which also exhibited distinct transcriptional profiles that suggest a competitive advantage in the presence of aminoglycosides.
Currently, there are no particularly effective biomarkers to distinguish between latent tuberculosis infection (LTBI) and active pulmonary tuberculosis (PTB) and evaluate the outcome of TB treatment. In this study, we have characterized the changes in the serum metabolic profiles caused by (Mtb) infection and standard anti-TB treatment with isoniazid-rifampin-pyrazinamide-ethambutol (HRZE) using GC-MS and LC-MS/MS. Seven metabolites, including 3-oxopalmitic acid, akeboside ste, sulfolithocholic acid, 2-decylfuran (4,8,8-trimethyldecahydro-1,4-methanoazulen-9-yl)methanol, d-(+)-camphor, and 2-methylaminoadenosine, were identified to have significantly higher levels in LTBI and untreated PTB patients (T0) than those in uninfected healthy controls (Un).
View Article and Find Full Text PDFBackground: Obstructive sleep apnea (OSA) is highly prevalent, but frequently undiagnosed. The existing biomarkers of OSA are relatively insensitive and inaccurate. Long non-coding RNAs (lncRNAs) have no protein-coding ability but have a role in regulating gene expression.
View Article and Find Full Text PDFand association with ethambutol resistance in : A multiomics analysis.
Front Cell Infect Microbiol
September 2022
Ethambutol (EMB) is a first-line antituberculosis drug currently being used clinically to treat tuberculosis. Mutations in the embCAB operon are responsible for EMB resistance. However, the discrepancies between genotypic and phenotypic EMB resistance have attracted much attention.
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