Chiral Cobalt Nanocluster-Driven Chemiluminescent System for the Facile Discrimination of Carnitine Enantiomers with a Hydrogel-Assisted Strategy.

Facile chiral discrimination without relying on chromatographic techniques has long been a huge challenge due to the minimal differences in the physicochemical properties of enantiomers. Polysaccharide hydrogels with natural chiral selectivity can be promising materials for constructing chiral discrimination platforms. In this study, l-cysteine-induced cobalt nanoclusters (LC-CoNCs) were prepared and utilized as chiral nanozymes for promoting the chemiluminescent (CL) signal of a luminol-HO system.

Exploring the Efficacy of pH-Responsive Vancomycin/Ag/ZIF-8 Nanoparticles Modified with Hyaluronic Acid for Enhanced Antibacterial Therapy and Wound Healing.

This study introduces a novel type of metal-organic framework material, specifically zeolitic imidazolate framework-8 (ZIF-8), that is integrated with silver nanoparticles and further enhanced by incorporating vancomycin (Van) to create a composite named as Van/Ag/ZIF-8. This composite demonstrates a potent and smart antimicrobial effect due to its ability for releasing silver ions and Van in a controlled manner, especially in the microacidic conditions surrounding bacterial cells. Furthermore, we used hyaluronic acid to modify Van/Ag/ZIF-8, resulting in a composite denoted as Van/Ag/ZIF-8@HA.

Robust Luminescent Pyrene-Based Metal-Organic Framework Hydrogel as a pH-Responsive Fluorescence Emitter for Sensitive Immunoassay of Cardiac Troponin I.

Despite many luminescent advantages including outstanding absorption coefficient and high quantum yield, pyrene and its derivatives have been suffering from a dramatic aggregation-caused quenching (ACQ) effect. Although the dramatic ACQ effect of pyrene-based fluorophores has been restrained in pyrene-doped metal-organic frameworks (MOFs), the low loading of fluorescent (FL) units substantially impedes the improved luminescent behaviors. Herein, pyrene-based MOFs hydrogel was synthesized with a high loading of pyrene as the unique organic linker blocks instead of a dopant in MOFs.

Multivalent Pt/TiCTx Nanocomposite-Based Immunochromatographic Sensor for Colorimetric/Temperature/Pressure Trimodal Detection.

Multimodal immunochromatographic sensors (ICSs) have acquired extensive attention since they not only provide reliable results by comparing the different output signals but also flexibly respond to various application environments. Herein, an ICS with triple signal outputs including colorimetry, temperature, and pressure was developed for sensitive detection of chlorothalonil. The multivalent Pt/TiCTx nanoparticles as signal tags were facilely synthesized by loading PtNPs onto single-layer TiCTx nanosheets with high surface area.

Hybrid Mn Atomic Clusters/Single-Dispersed Atoms with Dual Antioxidant Activities for a Chemiluminescent Immunoassay.

Single-dispersed atoms (SDAs) as catalysts have drawn extensive attention due to their ultimate atom utilization efficiency and desirable catalytic capability. Atomic clusters (ACs) with potential multiple enzyme-like activities also display great practicability in catalysis-based biosensing. In this work, hybrid Mn ACs/SDAs were implanted in the frameworks of defect-engineered MIL 101(Cr) modulated by excess acetic acid, with a high loading capability of 13.

Screening Probiotics for Anti- and Investigating the Effect of Probiotics on Patients with Infection.

Probiotics are natural microbial agents with beneficial properties such as bacteriostatic and anti-infective properties. Q21, Q25 and QA85, were isolated from the Chinese specialty fermented food "Jiangshui" and proved to be highly resistant to ( < 0.0001).

Functional fractions of Astragalus polysaccharides as a potential prebiotic to alleviate ulcerative colitis.

Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine that is significantly influenced by an imbalance in the gut microbiota. Astragalus membranaceus, particularly its polysaccharide components, has shown therapeutic potential for the treatment of UC, although the specific active constituents and their mechanistic pathways remain to be fully elucidated. In this study, we investigated two molecular weight fractions of Astragalus polysaccharides (APS), APS1 (Mw < 10 kDa) and APS2 (10 kDa < Mw < 50 kDa), isolated by ultrafiltration, focusing on their prebiotic effects, effects on UC, and the underlying mechanism.

Dual-Mechanism-Driven Ratiometric Electrochemiluminescent Biosensor for Methicillin-Resistant .

Design of a ratiometric method is a promising pathway to improve the sensitivity and reliability of electrochemiluminescent (ECL) assay, for which the signals produced at two distinct potentials change reversely as it is applied to the target analyte. Herein, a biosensor for ECL assay of methicillin-resistant (MRSA) was constructed by immobilizing porcine IgG for capturing MRSA onto an electrode that was precoated with β-cyclodextrin-conjugated luminol nanoparticles (β-CD-Lu NPs) as an anodic luminophore. MOF PCN 224 loaded with an atomically distributed Zn element (PCN 224/Zn) was conjugated with phage recombinant cellular-binding domain (CBD) to act as a cathodic luminophore for tracing MRSA.

Preparation of Co dual atomic site catalysts loaded on defect-engineered MOFs material with superb chemiluminescent enhancement effect for sensitive detection of bacteria.

Background: Dual atomic site catalysts (DASCs) have aroused extensive interest in analytical chemistry on account of the superb catalytic activity caused by the highly-exposed active centers and synergistic effect of adjacent active centers. The reported protocols for preparing DASCs usually involve harsh conditions such as acid/base etching and high-temperature calcination, leading to unfavorable water dispersity and restricted application. It is crucial to develop DASCs with satisfactory water dispersity, improved stability, and mild preparation procedures to facilitate their application as signal probes in analytical chemistry.

Paper-based analytical device integrated with bacteriophage tail fiber protein for bacteria detection and antimicrobial susceptibility test.

Severe antimicrobial resistance calls for developing rapid, sensitive and affordable methodological platform for clinical diagnosis of bacterial infection. Herein, a paper-based analytical device (PAD) for fluorescent (FL) detection and antimicrobial susceptibility test (AST) of Pseudomonas aeruginosa (P. aeruginosa) was fabricated by defining 96 hydrophilic microzones on a filter paper using a handheld stamp dipped with liquid wax.

PEGylated Ni Single-Atom Catalysts as Ultrasensitive Electrochemiluminescent Probes with Favorable Aqueous Dispersibility for Assaying Drug-Resistant Pathogens.

Ni single-atom catalysts (SACs) were synthesized by high-temperature calcination of nickel ions and 1,10-phenanthroline on carbon black as a carrier. Benefiting from the ultrahigh atom utilization efficiency, Ni SACs can significantly accelerate decay of dissolved oxygen to generate abundant reactive oxygen species through an oxygen reduction reaction occurring on cathodes. The generated reactive oxygen species can vastly enhance the electrochemiluminescent (ECL) signal of luminol without participation of exogenous co-reactants.

Small ubiquitin-related modifier-fused bacteriophage tail fiber protein with favorable aqueous solubility for lateral flow assay of Pseudomonas aeruginosa.

Rapid and sensitive assay of pathogenic bacteria is critical for minimizing the risk of infectious diseases. Inspired by the interaction between bacteriophages and host bacteria, we obtained a gene sequence of tail fiber protein (TFP) from Pseudomonas aeruginosa (P. aeruginosa) bacteriophage.

An electrochemical immunosensor coupling a bamboo-like carbon nanostructure substrate with toluidine blue-functionalized Cu(ii)-MOFs as signal probes for a C-reactive protein assay.

In this paper, a novel sandwich immunosensor based on a toluidine blue (Tb) loaded metal organic framework (Cu(ii)-HKUST-1/Tb) as the signal element and a nitrogen-doped 3D carbon nanostructure as the electrode substrate was constructed for the detection of C-reactive protein (CRP). Tb as an electrochemically active agent usually forms a polymer by aggregation in the solvent, causing a poor electrochemical response. Therefore, in order to overcome this obstacle, Cu(ii)-HKUST-1 with a porous nanostructure and large specific surface area as a carrier could adsorb a large number of Tb molecules on its surface to improve its electrochemical performance.

Electrochemical detection of C-reactive protein using functionalized iridium nanoparticles/graphene oxide as a tag.

C-reactive protein (CRP) has become a recognized indicator of inflammation. CRP concentration in serum is an important indicator for monitoring early heart damage, and it is also a newly discovered coronary heart disease-associated inflammatory factor. A conductive nano-hybrid material composed of Au NPs and ionic liquid functionalized molybdenum disulfide (Au NPs/IL-MoS) was prepared and utilized to immobilize primary CRP antibodies.

An Enzyme-Linked Immunosorbent Assay for C-Reactive Protein Based on Gold Nanoparticles@Metal Porphyrin Porous Compound.

In this paper, an enzyme-linked immunosorbent assay (ELISA) using gold nanoparticles/metal porphyrin porous compound (Au NPs@PAF-40-Fe) as a marker was developed. Quantitative detection of C-reactive protein (CRP) was achieved by UV-visible spectrophotometry under optimal conditions. The sensor has a good linear relationship in the range of 0.

2D-porphrinic covalent organic framework-based aptasensor with enhanced photoelectrochemical response for the detection of C-reactive protein.

In this study, a novel photoelectrochemical (PEC) aptasensor based on two-dimensional (2D) porphyrinic covalent organic frameworks (p-COFs) for the label-free detection of C-reactive protein (CRP) is presented. The obtained p-COFs possess high conductivity and an improved stability due to strong and rigid covalent linkages. The introduction of p-COFs hinder the recombination of electrons and holes, decreasing their band gap (E), thereby which improved the photocurrent conversion efficiency.

The Preparation of C-Reactive Protein Immunosensor Based on Nano-Mimetic Enzyme Co₃O₄.

In this work, a nano-mimetic enzyme Co3O4 nanoparticles with catalytic effect on the reduction of H2O2, was synthesized and used to label C-reactive protein antibody. Au nanoparticles (Au NPs) were loaded on COF-TpPa-1 to form Au NPs@COF-TpPa-1 with good biocompatibility and adsorption ability. A sandwich C-reactive protein immunosensor was prepared using Au NPs @COF-TpPa-1 as the immobilization matrix.