[The study of expression of BRMS1 gene protein and the expression of BRMS1 gene promotor area methylation in supraglottic laryngeal carcinoma and its clinical significance].

Objective: To investigate the expression of breast cancer metastasis suppressor 1 (BRMS1) gene protein and the expression of BRMS1 gene promotor area methylation in supraglottic cancer and to evaluate its clinical significance.

Method: The expression of BRMS1 protein and BRMS1 gene promotor area methylation were examined by using Western blotting method and methylation-specific polymerase chain reaction(MSP) method in 70 cases of supraglottic cancer tissues and 60 cases of their surrounding laryngeal normal mucosa tissues (LNT) and 44 cases of cervical lymph node metastasis of supraglottic cancer.

Result: Western blot results indicate that BRMS1 protein expression is declined expression level in supraglottic cancer tissue than the expression of BRMS1 protein in LNT of supraglottic cancer.

[Application of epiglottis with sternohyoid muscle in the surgery for laryngeal cancer].

Objective: To explore and evaluate the application of epiglottis with sternohyoid muscle in the surgery for laryngeal cancer.

Methods: Two fifty patients with laryngeal cancer were treated by partial laryngectomy and the laryngeal defects were reconstructed by epiglottic flap and sternohyoid muscle fascia flap. The staging of tumors: T2N0M0 23 cases, T3N1M0 14 cases, T3N2M0 13 cases.

[Expression and significance of nuclear factor kappaB in laryngeal carcinoma].

Objective: To explore the expression and significance of NF-kappaB in laryngeal carcinoma.

Method: The protein expression of NF-kappaB p65 in laryngeal carcinoma tissue and normal laryngeal tissue was examined by Western blot; NF-kappaB DNA binding activity was examined by electrophoretic motility shift assay (EMSA) in 20 laryngeal carcinoma tissue and 12 normal laryngeal tissue.

Result: The levels of NF-kappaB p65 protein and NF-kappaB DNA binding activity in the laryngeal carcinoma tissue were higher than those in normal laryngeal tissue.

[A study on the application of Nickle-Titanium alloys stent for prevention and treatment tracheostomal stenosis after total laryngectomy].

Objective: To evaluate the effect of Nickle-Titanium alloys annular stent to prevent and treatment tracheostomal stenosis after total laryngectomy.

Method: The stent was used following total laryngectomy and after total laryngectomy.

Result: Long stent was implanted in 2 cases following total laryngectomy and in 1 cases after total laryngectomy, Which resulted in widening stenosis and relieving obstruction of airway.

[Experimental and clinical study on the chemosensitivity assay in vitro for head and neck tumor by AgNOR methods].

Objective: To evaluate in vitro chemosensitivity of head and neck tumor to eight kinds of antineoplastic drugs by AgNOR technique.

Method: Fifty-nine cases' fresh specimens were obtained from head and neck tumors. We severed tumor tissue with simple mechanical separation method.