Low cost, portable, 3D printable tissue precision slicer.

Slicing tissue samples into thin pieces is commonly used in histology analysis and more recently for organotypic culture when tissue samples are sliced alive. Currently available devices for slicing tissue samples are either designed for fixed tissue samples at low cryogenic temperatures (, Cryostats), or bulky and expensive (, vibratome), preventing them from routine lab usage. Here we report a cost-effective device designed to section live tissues for subsequent culture.

The Electroencephalogram (EEG) Study for Estimating Endurance Sports Performance Base on Eigenvalues Extraction Method.

Objectives: Brain-behavior connections are a new means to evaluate sports performance. This electroencephalogram (EEG) study aims to estimate endurance exercise performance by investigating eigenvalue trends and comparing their sensitivity and linearity.

Methods: Twenty-three cross-country skiers completed endurance cycling tasks.

Cell type specification and diversity in subpallial organoids.

Neural organoids have emerged as valuable tools for studying the developing brain, sparking enthusiasm and driving their adoption in disease modeling, drug screening, and investigating fetal neural development. The increasing popularity of neural organoids as models has led to a wide range of methodologies aimed at continuous improvement and refinement. Consequently, research groups often improve and reconfigure protocols to create region-specific organoids, resulting in diverse phenotypes, including variations in morphology, gene expression, and cell populations.

The recent advances of MnFeO-based nanoparticles in environmental application: A review.

The manganese ferrite (MnFeO)-based nanoparticles showed a substantial potential to remediate the various pollutants in environmental application due to low cost, simple magnetic separation and high removal capacity. Herein, the functionalization of various MnFeO-based nanoparticles was briefly summarized; Then the recent advances concerning the removal of pollutants (i.e.

Recent advances of application of bentonite-based composites in the environmental remediation.

Bentonite-based composites have been widely utilized in the removal of various pollutants due to low cost, environmentally friendly, ease-to-operate, whereas the recent advances concerning the application of bentonite-based composites in environmental remediation were not available. Herein, the modification (i.e.

Graphene-integrated mesh electronics with converged multifunctionality for tracking multimodal excitation-contraction dynamics in cardiac microtissues.

Cardiac microtissues provide a promising platform for disease modeling and developmental studies, which require the close monitoring of the multimodal excitation-contraction dynamics. However, no existing assessing tool can track these multimodal dynamics across the live tissue. We develop a tissue-like mesh bioelectronic system to track these multimodal dynamics.

Patterning ganglionic eminences in developing human brain organoids using a morphogen-gradient-inducing device.

In early neurodevelopment, the central nervous system is established through the coordination of various neural organizers directing tissue patterning and cell differentiation. Better recapitulation of morphogen gradient production and signaling will be crucial for establishing improved developmental models of the brain in vitro. Here, we developed a method by assembling polydimethylsiloxane devices capable of generating a sustained chemical gradient to produce patterned brain organoids, which we termed morphogen-gradient-induced brain organoids (MIBOs).

Rapid cell isolation in breastmilk in a non-clinical setting by a deterministic lateral displacement device and selective water and fat absorption.

Breastmilk is a reliable source of biomarker-containing, sloughed breast cells that have the potential to give valuable health insights to new mothers. Furthermore, known DNA-based markers for pregnancy-associated breast cancer are chemically stable and can be safely stored on a commercially available FTA® Elute Micro (EM) card, which can subsequently be mailed to a testing facility for the cost of a stamp. In theory, this archiving process can be performed by nonprofessionals in very low-resource settings as it simply requires placing a drop of breastmilk on an EM card.

Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes.

Cells continuously experience and respond to different physical forces that are used to regulate their physiology and functions. Our ability to measure these mechanical cues is essential for understanding the bases of various mechanosensing and mechanotransduction processes. While multiple strategies have been developed to study mechanical forces within two-dimensional (2D) cell culture monolayers, the force measurement at cell-cell junctions in real three-dimensional (3D) cell models is still pretty rare.

Directional Cell Migration Guided by a Strain Gradient.

Strain gradients widely exist in development and physiological activities. The directional movement of cells is essential for proper cell localization, and directional cell migration in responses to gradients of chemicals, rigidity, density, and topography of extracellular matrices have been well-established. However; it is unclear whether strain gradients imposed on cells are sufficient to drive directional cell migration.

A method of gas sensor drift compensation based on intrinsic characteristics of response curve.

Sensor drift, which is an inevitable and challenging problem in gas sensing, seriously affects the detection performance of sensor. In this study, a new sensor drift compensation method, which is based on intrinsic characteristic of sensory response, is proposed. The dataset of gas sensor for two types of gas with a period of 36 months are collected and two features (one is steady-state feature, another is transient feature) are extracted.

Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids.

De novo mutations and copy number deletions in NRXN1 (2p16.3) pose a significant risk for schizophrenia (SCZ). It is unclear how NRXN1 deletions impact cortical development in a cell type-specific manner and disease background modulates these phenotypes.

Spectroscopic and modeling investigation of U(VI) removal mechanism on nanoscale zero-valent iron/clay composites.

Nanoscale zero-valent iron (nZVI)-based composites have been widely utilized in environmental cleanup due to their low cost, high adsorption performance and strong redox activity. Herein, removal mechanism of U(VI) on nZVI/clay composites was demonstrated by batch, XPS and modeling techniques. The batch experiments showed that nZVI/clay composites exhibited the high removal capacity (88.

Clinicopathological and CT features of tumor spread through air space in invasive lung adenocarcinoma.

Objective: Tumor spread through air spaces (STAS) has recently been reported as a novel invasive pattern in lung adenocarcinoma. Thus, this study aimed to investigate the clinicopathological and radiological features in invasive lung adenocarcinoma with tumor STAS.

Methods: Data of 503 invasive lung adenocarcinoma patients who underwent surgery between 1 January 2015 and 31 December 2021 were collected.

Application of Surface Electromyography in Exercise Fatigue: A Review.

Exercise fatigue is a common physiological phenomenon in human activities. The occurrence of exercise fatigue can reduce human power output and exercise performance, and increased the risk of sports injuries. As physiological signals that are closely related to human activities, surface electromyography (sEMG) signals have been widely used in exercise fatigue assessment.

Bioinspired two-in-one nanotransistor sensor for the simultaneous measurements of electrical and mechanical cellular responses.

The excitation-contraction dynamics in cardiac tissue are the most important physiological parameters for assessing developmental state. We demonstrate integrated nanoelectronic sensors capable of simultaneously probing electrical and mechanical cellular responses. The sensor is configured from a three-dimensional nanotransistor with its conduction channel protruding out of the plane.

Boosting photocatalytic efficiency of MoS/CdS by modulating morphology.

CdS-based composites as the highly efficient photocatalyst have been extensively investigated in recent years due to the suitable band gap and high photocatalytic efficiency. In this study, the effect of various factors (pH, U(VI) concentration, contents, and types of photocatalyst) on photocatalytic reduction of U(VI) by MoS/CdS composite was investigated. The optimized experimental conditions (e.

Surface functionalization of poly(dimethylsiloxane) substrates facilitates culture of pre-implantation mouse embryos by blocking non-selective adsorption.

Poly(dimethylsiloxane) (PDMS) is widely used in biomedical settings such as microfluidics for its optical transparency, castability, gas permeability and relative biocompatibility. While PDMS devices with certain modifications or treatments have been used for mammalian pre-implantation embryo culture, it is unclear why native PDMS leads to significant embryo death. In this study, we employ Nile Red as a model hydrophobic small molecule to demonstrate that significant hydrophobic sequestration occurs on native PDMS substrates even with a bovine serum albumin-containing KSOM pre-equilibration.

Condensation tendency and planar isotropic actin gradient induce radial alignment in confined monolayers.

A monolayer of highly motile cells can establish long-range orientational order, which can be explained by hydrodynamic theory of active gels and fluids. However, it is less clear how cell shape changes and rearrangement are governed when the monolayer is in mechanical equilibrium states when cell motility diminishes. In this work, we report that rat embryonic fibroblasts (REF), when confined in circular mesoscale patterns on rigid substrates, can transition from the spindle shapes to more compact morphologies.

Quantifying tensile forces at cell-cell junctions with a DNA-based fluorescent probe.

Cells are physically contacting with each other. Direct and precise quantification of forces at cell-cell junctions is still challenging. Herein, we have developed a DNA-based ratiometric fluorescent probe, termed DNAMeter, to quantify intercellular tensile forces.

Quantitative and Multiplexed Fluorescence Lifetime Imaging of Intercellular Tensile Forces.

Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications. However, the precise measurement of intercellular forces is still quite challenging, especially considering the complex environment at cell-cell junctions. In this study, we report a fluorescence lifetime-based approach to image and quantify intercellular molecular tensions.