Functionalized gelatin/poly(l-lactide-co-ε-caprolactone) fibrous membrane promotes scarless wound healing by modulating inflammation and reducing fibrosis.

In cases of deep skin defects, spontaneous tissue regeneration and excessive collagen deposition can result in the formation of proliferative scarring. Salvianolic acid B (SAB) demonstrates promising applications in the treatment of fibrotic diseases, including scarring. This study prepared biodegradable gelatin (Gel) and poly(lactic-co-ε-caprolactone) (PLCL) nanofibrous membranes using electrospinning technology and functionalized them with salvianolic acid B to create fibrous membrane dressings with anti-scarring properties.

Biological characterization of novel O157:H7 phages and their bacteriostatic effects in milk and pork.

Foodborne bacteria, particularly () O157:H7, are significant contributors to foodborne illnesses, with antibiotic overuse exacerbating the issue through the emergence of multidrug-resistant strains. This study investigated the potential of phages in food safety, examining their biological traits and bacteriostatic properties. Two phages (vB_EcoP_SD2, vB_EcoP_SD6) of O157:H7 were isolated from slaughterhouse sewage and characterized for morphology, genomic composition, phage phylogenetic tree, optimal multiplicity of infection (MOI), one-step growth curve, thermal and pH stability and antibacterial efficacy.

Multienzyme-Activity Sulfur Quantum Dot Nanozyme-Mediated Cascade Reactions in Whole-Stage Symptomatic Therapy of Infected Bone Defects.

Integrating the therapeutic efficacy of early bacterial clearance, midstage inflammatory remission, and late-stage effective tissue healing is considered a pivotal challenge in symptomatic treatment of infected bone defects (IBDs). Herein, a microenvironment-adaptive nanoplatform based on a sulfur quantum dot (SQD) nanozyme was proposed for whole-stage symptomatic therapy of IBDs by mediating the sequence of enzyme cascade reactions. The SQD nanozyme prepared by a size-engineering modification strategy exhibits enhanced multienzyme activity compared to conventional micrometer- and nanometer-sized sulfur particles.

Top-down curing to construct self-retaining and moisture-pumping double-layered dressing with enhanced antibacterial, hemostatic, and wound healing performances.

Continuous microenvironment modulation is an ongoing challenge in wound dressing, which includes excessive exudate absorption, oxygen delivery, bacterial inhibition and angiogenesis. Herein, we developed an construction strategy to fabricate a self-retaining double-layered wound dressing, where the top layer precursor was composed of Ca-containing polyvinyl butyral (PVB) solution dispersed with hydroxypropyl methylcellulose (HPMC) particles, and the bottom one consisted of sodium alginate (Alg) solution blended with Ag-doped mesoporous bioactive glass powders (Ag-MBG). When in use, both precursors were simultaneously squeezed out from the twin nozzles connected to the individual chambers of a twin-chambered syringe, whereby Ca in the top layer rapidly migrated downwards to crosslink Alg in the bottom layer, leading to the formation of an Alg/Ag-MBG (AA) functional hydrogel for filling an irregular wound.

Equine Herpesvirus-1 Induced Respiratory Disease in Dezhou Donkey Foals: Case Study from China, 2024.

Equine herpesvirus-1 (EHV-1) is a significant pathogen that causes substantial economic losses in the equine industry worldwide, which leads to severe respiratory diseases and abortions in horses. However, reports of EHV-1 infection in donkeys are limited, particularly in China. This case study reported an EHV-1-induced respiratory disease in Dezhou donkey foals in Shandong Province, China, in July 2024.

Bacteriophage LDT325 enhances tolerance by improving antioxidant defense in tea plant [ (L.) O. Kuntze].

Bud blight caused by is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treated tea plants to bud blight was notably greater compared with that of the control group.

Characterization and genomic analysis of Abortusequi phage, vB_SalP_LDDK01, and its biocontrol application in donkey meat.

Abortusequi ( Abortusequi) is the primary cause of abortions in equine animals, and can cause serious foodborne illness. Thus, effective biocontrol strategies are needed to decontaminate and control the emergence of foodborne diseases. In recent years, phages have been used as a new strategy for modulating foodborne pathogens and food safety.

EGCG-Modified Bioactive Core-Shell Fibers Modulate Oxidative Stress to Synergistically Promote Vascularized Bone Regeneration.

Oxidative stress induced by reactive oxygen species (ROS) can adversely affect tissue repair, whereas endowing biomaterials with antioxidant activity can improve the in vivo microenvironment, thereby promoting angiogenesis and osteogenesis. Accordingly, this study utilized epigallocatechin-3-gallate (EGCG), a material known for its reducing properties, oxidative self-polymerization capability, and strong binding characteristics, to modify a bioactive core-shell fibrous membrane (10RP-PG). Compared to the 10RP-PG fibrous membrane, the EGCG-modified fibrous membrane (E/10RP-PG) exhibited superior hydrophilicity, excellent cell adhesion, and compatibility.

Integrated analysis of proteome and transcriptome revealed changes in multiple signaling pathways involved in immunity in the northern snakehead () during infection.

The northern snakehead () is a valuable aquaculture species across certain Asian countries, contributing significantly to economic prosperity and dietary needs. However, its productivity faces significant challenges, particularly from diseases such as nocardiosis, caused by . To date, the majority of research efforts have focused on describing the observed phenomena related to infection.

HSP60 inhibits DF-1 apoptosis through its mitochondrial signal peptide.

HSP60 is implicated in many biological functions and plays a key role in maintaining oxidative stress and preserving mitochondrial integrity. Our previous study showed that HSP60 inhibits apoptosis. In this study, we further investigated the mechanism of apoptosis inhibition by HSP60.

Protective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation.

Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses and donkeys, which is a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available to effectively treat EqHV-8 infection. Therefore, it is crucial to develop new antiviral approaches to prevent potential pandemics caused by EqHV-8.

The double-layer emulsions loaded with bitter melon (Momordica charantia L.) seed oil protect against dextran sulfate sodium-induced ulcerative colitis in mice.

In this study, a whey protein isolate (WPI)-chitooligosaccharide (COS) stabilized bitter melon (Momordica charantia L.) seed oil emulsions (WC-BSOE) were prepared using the electrostatic layer-by-layer self-assembly technique, and their modulating effects on ulcerative colitis (UC) were investigated in dextran sulfate sodium (DSS)-induced UC mice model. The stability and releasing ability of WC-BSOE under simulated gastrointestinal digestion condition and their acute toxicity were also investigated.

Enhanced polarization and endurance properties of ZrO-based ferroelectric capacitor using HfOinterfacial layer.

Recently discovered ferroelectricity in fluorite-structure ZrOthin film has attracted increasing and intense interest due to its lower crystallization temperature and higher content in nature in comparison to hafnium oxide. Here, the effect of HfOinterfacial layer on the ferroelectric properties of ZrOthin films is investigated systematically by designing four types of interfacial structures. It is revealed that the ferroelectric orthorhombic phase, remanent polarization, and endurance can be improved in ZrOthin film by inserting both a top- and bottom-HfOinterfacial layer.

Nano-laponite encapsulated coaxial fiber scaffold promotes endochondral osteogenesis.

Osteoinductive supplements without side effects stand out from the growth factors and drugs widely used in bone tissue engineering. Lithium magnesium sodium silicate hydrate (laponite) nanoflake is a promising bioactive component for bone regeneration, attributed to its inherent biosafety and effective osteoinductivity. Up to now, the osteogenic potential and mechanisms of laponite-encapsulated fibrous membranes remain largely unexplored.

Composite hydrogels with antioxidant and robust adhesive properties for the prevention of radiation-induced dermatitis.

Radiotherapy is a pivotal means of cancer treatment, but it often leads to radiation dermatitis, a skin injury caused by radiation-induced excess reactive oxygen species (ROS). Scavenging free radicals in the course of radiation therapy will be an effective means to prevent radiation dermatitis. This study demonstrates a novel double network hydrogel doped with MoS nanosheets for the prevention of radiation-induced dermatitis.

Enzyme-Activated Biomimetic Vesicles Confining Mineralization for Bone Maturation.

Inspired by the crucial role of matrix vesicles (MVs), a series of biomimetic vesicles (BVs) fabricated by calcium glycerophosphate (CaGP) modified polyurethane were designed to mediate the mineralization through enzyme activation for bone therapy. In this study, alkaline phosphatase (ALP) was harbored in the porous BVs by adsorption (Ad-BVs) or entrapment (En-BVs). High encapsulation of ALP on En-BVs was effectively self-activating by calcium ions of CaGP-modified PU that specifically hydrolyzed the organophosphorus (CaGP) to inorganic phosphate, thus promoting the formation of the highly oriented bone-like apatite .

Blebbistatin as a novel antiviral agent targeting equid herpesvirus type 8.

Introduction: Equid herpesvirus type 8 (EqHV-8) poses a significant threat to equine health, leading to miscarriages and respiratory diseases in horses and donkeys, and results in substantial economic losses in the donkey industry. Currently, there are no effective drugs or vaccines available for EqHV-8 infection control.

Methods: In this study, we investigated the and antiviral efficacy of Blebbistatin, a myosin II ATPase inhibitor, against EqHV-8.

In situ comparison of osteogenic effects of polymer-based scaffolds with different degradability by integrated scaffold model.

Polymer-based scaffolds with different degradability have been investigated to screen the matrix whose degradation rate is more closely matched with the bone regeneration rate. However, these comparisons are inclined to be compromised by the animal individual differences. In this study, we constructed an integrated scaffold model comprising four parts with different degradability and bioactivity to achieve an in situ comparison of bone regeneration ability of different scaffolds.

A Novel Strategy for Fabrication of Polyamide 66/Nanohydroxyapatite Composite Bone Repair Scaffolds by Low-Temperature Three-Dimensional Printing.

Due to the decomposition temperature of Polyamide 66 (PA66) in the environment is close to its thermoforming temperature, it is difficult to construct porous scaffolds of PA66/nanohydroxyapatite (PA66/HAp) by fused deposition modeling (FDM) three-dimensional (3D) printing. In this study, we demonstrated for the first time a method for 3D printing PA66/HAp composites at room temperature, prepared PA66/HAp printing ink using a mixed solvent of formic acid/dichloromethane (FA/DCM), and constructed a series of composite scaffolds with varying HAp content. This printing system can print composite materials with a high HAp content of 60 wt %, which is close to the mineral content in natural bone.

Rutin prevents EqHV-8 induced infection and oxidative stress via Nrf2/HO-1 signaling pathway.

Introduction: The Nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway has been extensively studied for its role in regulating antioxidant and antiviral responses. The Equid herpesvirus type 8 (EqHV-8) poses a significant threat to the equine industry, primarily manifesting as respiratory disease, abortions, and neurological disorders in horses and donkeys. Oxidative stress is considered a key factor associated with pathogenesis of EqHV-8 infection.