Publications by authors named "Yuanzhang Sun"
Article Synopsis
- The combination of the Embden-Meyerhof pathway (EMP) and the 2-C-methyl-D-erythritol 4-phosphate pathway (MEP) in E. coli faces challenges due to an uneven production of pyruvate and glyceraldehyde-3-phosphate (G3P).
- Experiments showed that the Entner-Doudoroff Pathway (EDP) is the most effective for isoprene production compared to other glycolytic pathways, as it produces pyruvate and G3P at the same time, resulting in isoprene yields over three and six times higher than those from the EMP.
- The study highlights that combining EDP with the Pentose Phosphate Pathway (PPP)
In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain.
View Article and Find Full Text PDFBiosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway.
Appl Microbiol Biotechnol
June 2011
Isoprene is an aviation fuel of high quality and an important polymer building block in the synthetic chemistry industry. In light of high oil prices, sustained availability, and environmental concerns, isoprene from renewable materials is contemplated as a substitute for petroleum-based product. Escherichia coli with advantages over other wild microorganisms, is considered as a powerful host for biofuels and chemicals.
View Article and Find Full Text PDF