An activity-based sensing fluorogenic probe for monitoring O-methyltransferase in plants.

Activity-based sensing probes are powerful tools for monitoring enzymatic activities in complex biological samples such as cellular and live animals; however, their application in plants remains challenging. Herein, fourteen activity-based fluorescent probes were assayed against Arabidopsis O-methyltransferases (AtOMTs). One probe, 3-BTD, displayed a high selectivity, reactivity, and fluorescence response toward AtOMTs especially the isoform AtCCoAOMT.

Magnetic Covalent Organic Framework for Efficient Solid-Phase Extraction of Uranium for on-Site Determination by Portable X-ray Fluorescence Spectrometry.

Uranium plays a pivotal role in the nuclear industry; however, its inadvertent release has raised concerns regarding health and environmental implications. It is crucial for a prompt warning and accurate tracing of uranium contamination in emergency scenarios. In this study, a novel and simple method was proposed that combines magnetic dispersive solid-phase extraction (MDSPE) with portable X-ray fluorescence spectrometry (XRF) for the on-site sampling and determination of trace uranium in real samples.

Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 DCs.

The induction of a robust CD8 T cell response is critical for the success of an antiviral vaccine. In this study, we incorporated a STING agonist (SA) 2'3'-cGAMP into a previously developed exosome-based CVB3 viral myocarditis vaccine (Exo-VP1) to enhance its ability to induce CD8 T cell responses and immunoprotection. Our results showed that compared to free SA adjuvant, exosome-mediated co-delivery (Exo-VP1) significantly enhanced SA uptake by dendritic cells (DCs) and more potently stimulated DC maturation.

Prevalence and characterization of Staphylococcus aureus and Staphylococcus argenteus isolated from rice and flour products in Guangdong, China.

Staphylococcus aureus and Staphylococcus argenteus have been implicated in food poisoning outbreaks, and have been found in various types of food products according to our previous study. Rice and flour products are popular and widely consumed around the world. However, limited data are available on the microbial safety of S.

Occurrence, Antibiotic Susceptibility, Biofilm Formation and Molecular Characterization of Isolated from Raw Shrimp in China.

The aim of this study was to determine the prevalence and characterization of isolated from 145 shrimp samples from 39 cities in China. The results show that 41 samples (28%) from 24 cities were positive, and most of the positive samples (39/41, 95.1%) were less than 110 MPN/g.

The Association between a Minimum Amount of Physical Activity and Subsequent Muscle Strength and Balance in Older Adults: A Prospective Study.

This study aimed to investigate the association between 15 min of daily moderate-to-vigorous physical activity (MVPA) and subsequent muscle strength and balance in older adults. Data concerning community-dwelling older Taiwanese adults (mean age, 69.5 years) were collected during the baseline period (2018) and at a 12-month follow-up survey (2019).

Exosome-based delivery of VP1 protein conferred enhanced resistance of mice to CVB3-induced viral myocarditis.

Coxsackievirus B3 (CVB3) is an important cause of viral myocarditis with no vaccine available in clinic. Herein we constructed an exosome-based anti-CVB3 vaccine (Exo-VP1), and compared its immunogenicity and immunoprotection with our previously reported recombinant VP1 protein (rVP1) vaccine. We found that compared with the 25 μg rVP1 vaccine, Exo-VP1 vaccine containing only 2 μg VP1 protein induced much stronger CVB3-specific T cell proliferation and CTL responses (with an increase of more than 70% and 40% respectively), and elicited greater splenic Th1/CTL associated cytokines (IFN-γ, TNF-α and IL-12).

The emergence of novel macrolide resistance island in Macrococcus caseolyticus and Staphylococcus aureus of food origin.

Food-derived Staphylococcaceae species with severe antimicrobial resistance, especially Staphylococcus aureus, is a major threat to public health. Macrococcus caseolyticus (M. caseolyticus) is a member of the Staphylococcaceae family which plays a vital role in fermented products and disease causation in animals.

[Advances in plant caffeic acid--methyltransferase].

Monolignin alcohols (type H, type G and type S) are the basic units of lignin and lignans in plants, and their composition differences directly determine the chemical diversity and biological activity of lignin and lignans. Caffeic acid -methyltransferase (COMT) catalyzes the methylation of oxygen atoms on the hydroxyl groups of phenylpropanoids, playing a critical role in the composition of different types of monolignin alcohols, and thus acting as a key enzyme involved in the biosynthesis pathway of lignin and lignans. A previous review published in 2010 mainly introduced the gene characteristics of COMT and its regulatory role in lignin biosynthesis.

Isatis indigotica: from (ethno) botany, biochemistry to synthetic biology.

Isatis indigotica Fort. (Chinese woad) is a species with an ancient and well-documented history as an indigo dye and medicinal plant. It is often confused with Isatis tinctoria L.