A Chemiluminescent Hydrogen Sulfide Probe with Multimodal Validation for Inflammation Diagnosis in Vivo.

Probes capable of simultaneously detecting and differentiating biothiols is crucial yet challenging in optical chemical sensing. In this study, we design a multimodal probe, CL-NBD, through integrating chemiluminescence (high sensitivity and signal-to-noise ratio), absorption (visual discrimination by the naked eye), and fluorescence to distinguish various biothiol species. CL-NBD exhibits distinct signal response modes for hydrogen sulfide (HS), cysteine/homocysteine (Cys/Hcy), and glutathione.