High-throughput screening method for glycolipids based on substrate modification and their efficient biomanufacturing.

The development of a high-throughput screening (HTS) method is crucial for boosting microbial synthesis. However, in non-model strains, genetic editing-based HTS is often not feasible. Here, a substrate-modified HTS strategy using Starmerella bombicola PL0120, a sophorolipids (SLs)-producing strain, was designed.

4D-Printed Magnetic Responsive Bilayer Hydrogel.

Despite its widespread application in targeted drug delivery, soft robotics, and smart screens, magnetic hydrogel still faces challenges from lagging mechanical performance to sluggish response times. In this paper, a methodology of in situ generation of magnetic hydrogel based on 3D printing of poly-N-isopropylacrylamide (PNIPAM) is presented. A temperature-responsive PNIPAM hydrogel was prepared by 3D printing, and FeO magnetic particles were generated in situ within the PNIPAM network to generate the magnetic hydrogel.

Bivalent SMAC mimetic APG-1387 reduces HIV reservoirs and limits viral rebound in humanized mice.

Latent viral reservoirs (VRs) represent a main barrier to HIV cure. Thus, developing new approaches that can purge and eliminate VRs paves the path toward achieving an HIV-1 cure. APG-1387, a bivalent SMAC mimetic (SM), efficiently reactivates latent HIV expression in T cell line models and enhances active caspase 3 expression, a condition that typically leads to apoptosis.

Spatial-temporal characteristics and the importance of environmental factors in relation to algal blooms in coastal seas.

In the prediction of algal blooms with the artificial intelligence (AI) technology, the proper selection of the indices is crucial to the quality of the results. Although eutrophication and climate change have been identified as the main contributors to algal blooms, limited knowledge of the relationships among environmental factors hinders the accurate and correct AI prediction. To explore the importance of environmental factors in the occurrence of algal blooms in coastal seas and facilitate the selection of inputs for the prediction model, this study examines observed water quality data and analyzes their relationship using machine learning methods.

A Multi-task Neural Network for Image Recognition in Magnetically Controlled Capsule Endoscopy.

Background And Aims: Physicians are required to spend a significant amount of reading time of magnetically controlled capsule endoscopy. However, current deep learning models are limited to completing a single recognition task and cannot replicate the diagnostic process of a physician. This study aims to construct a multi-task model that can simultaneously recognize gastric anatomical sites and gastric lesions.

Variations in water exchange in the sub-areas of a bay following large-scale land reclamation.

Semi-enclosed bays often have weak water exchange capacities, leading to frequent environmental pollution, particularly localized pollution. This study examines the local effects of changes in local factors on water circulation within Bohai Bay after land reclamation. To address the limitations of previous methods in measuring sub-regional water exchange, we introduce the concept of Local Average Influence Time (LAIT) to qualitatively and quantitatively analyze the impact of land reclamation on water exchange between sub-regions in semi-enclosed bays.

Polystyrene microplastics as carriers for nano-hydroxyapatite particles: Impact of surface functionalization and mechanistic insights.

The potential of microplastics (MPs) to act as carriers for contaminants or engineered nanomaterials is of rising concern. However, directly determining the vector effect of polystyrene (PS) MPs towards nano-hydroxyapatite (nHAP) particles, a typical nano phosphorus fertilizer and soil remediation material, has been rarely studied. In this study, the interaction of differentially surface functionalized PS MPs with nHAP were investigated through batch experiments under different solution chemistry conditions.

CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii.

Komagataella phaffii, a nonconventional yeast, is increasingly attractive to researchers owing to its posttranslational modification ability, strict methanol regulatory mechanism, and lack of Crabtree effect. Although CRISPR-based gene editing systems have been established in K. phaffii, there are still some inadequacies compared to the model organism Saccharomyces cerevisiae.

Developmental role of macrophages modeled in human pluripotent stem cell-derived intestinal tissue.

Macrophages populate the embryo early in gestation, but their role in development is not well defined. In particular, specification and function of macrophages in intestinal development remain little explored. To study this event in the human developmental context, we derived and combined human intestinal organoid and macrophages from pluripotent stem cells.

Quantifying global colonization pressures of alien vertebrates from wildlife trade.

The global trade in live wildlife elevates the risk of biological invasions by increasing colonization pressure (the number of alien species introduced to an area). Yet, our understanding of species traded as aliens remains limited. We created a comprehensive global database on live terrestrial vertebrate trade and use it to investigate the number of traded alien species, and correlates of establishment richness for aliens.

Effects of Fermentation on Bioactivity and the Composition of Polyphenols Contained in Polyphenol-Rich Foods: A Review.

Polyphenols, as common components with various functional activities in plants, have become a research hotspot. However, researchers have found that the bioavailability and bioactivity of plant polyphenols is generally low because they are usually in the form of tannins, anthocyanins and glycosides. Polyphenol-rich fermented foods (PFFs) are reported to have better bioavailability and bioactivity than polyphenol-rich foods, because polyphenols are used as substrates during food fermentation and are hydrolyzed into smaller phenolic compounds (such as quercetin, kaempferol, gallic acid, ellagic acid, etc.

knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors.

Introduction: Natural Killer (NK) cells hold the potential to shift cell therapy from a complex autologous option to a universal off-the-shelf one. Although NK cells have demonstrated efficacy and safety in the treatment of leukemia, the limited efficacy of NK cell-based immunotherapies against solid tumors still represents a major hurdle. In the immunosuppressive tumor microenvironment (TME), inhibitory interactions between cancer and immune cells impair antitumoral immunity.

Interaction of glycine with Li in the (HO) (n = 0-8) clusters.

Context: We investigated the interaction between glycine and Li in water environment based on the Gly·Li(HO) (n = 0-8) cluster. Our study shows that for Gly·Li, Li binds to both carbonyl oxygen and amino nitrogen to form a bidentate structure, and the first three water molecules preferentially interact with Li. For n = 0-5, the complexes of Gly·Li(HO) exist in neutral form, and when the water number reached 6, the complex can coexist in neutral and zwitterionic form, then zwitterionic structures are dominant for n = 7, 8.

Immobilized glucoamylase based on ZIF-8: Preparation, response surface optimization, characterization.

The glucoamylase@ZIF-8 was prepared using ZIF-8 material as the carrier in this study. The preparation process was optimized by response surface methodology, and the stability of glucoamylase@ZIF-8 was determined. The material was characterized by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy.

Estimation of nutrient (N and P) fluxes into Newark Bay, USA.

This study was conducted in northern New Jersey, USA, to estimate the nutrient fluxes from the Passaic River, the Hackensack River and other sources into Newark Bay and the nutrient residence time in Newark Bay. Bi-weekly total inorganic nitrogen (TIN) and orthophosphate concentration data in the Passaic River, the Hackensack River, and Newark Bay for over 15 years (2004-2019) were collected along with daily river discharge data from the public database. The annual TIN and orthophosphate (ortho-P) loading from the Passaic River ranged from 915 × 10 kg y to 251 × 10 kg y and 94 × 10 kg yto 372 × 10 kg y, respectively.

Convolutional neural network-based segmentation network applied to image recognition of angiodysplasias lesion under capsule endoscopy.

Background: Small intestinal vascular malformations (angiodysplasias) are common causes of small intestinal bleeding. While capsule endoscopy has become the primary diagnostic method for angiodysplasia, manual reading of the entire gastrointestinal tract is time-consuming and requires a heavy workload, which affects the accuracy of diagnosis.

Aim: To evaluate whether artificial intelligence can assist the diagnosis and increase the detection rate of angiodysplasias in the small intestine, achieve automatic disease detection, and shorten the capsule endoscopy (CE) reading time.

Generation of functional human T cell development in NOD/SCID/IL2rγ humanized mice without using fetal tissue: Application as a model of HIV infection and persistence.

Humanization of mice with functional T cells currently relies on co-implantation of hematopoietic stem cells from fetal liver and autologous fetal thymic tissue (so-called BLT mouse model). Here, we show that NOD/SCID/IL2rγ mice humanized with cord blood- derived CD34 cells and implanted with allogeneic pediatric thymic tissues excised during cardiac surgeries (CCST) represent an alternative to BLT mice. CCST mice displayed a strong immune reconstitution, with functional T cells originating from CD34 progenitor cells.

Preparation and characterization of glucoamylase microcapsules prepared by W/O/W type complex coacervation freeze drying.

Glucoamylase was often used in the brewing industry but was unstable to several environmental factors and reacted quickly to produce fermentable sugar, which limited its applications. Microencapsulation could effectively overcome the drawbacks. This study evaluated the feasibility of the preparation of glucoamylase microcapsules (GM) using W/O/W complex coacervation-freeze-drying method.

Autologous humanized mouse models of iPSC-derived tumors enable characterization and modulation of cancer-immune cell interactions.

Modeling the tumor-immune cell interactions in humanized mice is complex and limits drug development. Here, we generated easily accessible tumor models by transforming either primary skin fibroblasts or induced pluripotent stem cell-derived cell lines injected in immune-deficient mice reconstituted with human autologous immune cells. Our results showed that fibroblastic, hepatic, or neural tumors were all efficiently infiltrated and partially or totally rejected by autologous immune cells in humanized mice.

Assessing the contribution of odor-active compounds in icewine considering odor mixture-induced interactions through gas chromatography-olfactometry and Olfactoscan.

The sensory impact of odor-active compounds on icewine aroma could be influenced by perceptual interactions with other odor-active compounds. The aim of this study was to establish an approach to evaluate the contribution of odor-active compounds found in icewine considering mixture-induced perceptual interactions. By comparing the impact of key odorants detected in icewine following a gas chromatography-olfactometry approach with an Olfactoscan-based methodology using a background odor of icewine, 69 odor zones were detected, and their related compounds were further identified.

Sedimentary organic carbon and nutrient distributions in an endorheic lake in semiarid area of the Mongolian Plateau.

Due to the lack of outlets, inflowing pollutants are often deposited in an endorheic lake, posing potential pressure on the environment. With climate change, extreme weather is expected to be more frequent and will contribute to the release of carbon and nutrients buried in the lakebeds. However, the distribution of sedimentary organic carbon and nutrients and the mechanisms that control the distribution are not fully understood, despite their significance to environmental development in endorheic lakes being widely recognized.

Trained immunity induced by in vivo peptide-based STAT6 inhibition prevents ragweed allergy in mice.

Background: Trained immunity is the ability of the innate immune system to form immune memory responses to provide support the formation of appropriate adaptive responses. Allergic airways disease (AAD) is a maladapted immune response to allergens, initiated and maintained by the type 2 (T2) inflammatory pathway. It is predicated by the elaboration of cytokines IL-4 and IL-13 and follows activation of the STAT6 transcription factor.

Penisarins A and B, Sesquiterpene Coumarins Isolated from an Endophytic sp.

Penisarins A () and B (), sesquiterpene coumarins with an unusual tricyclic sesquiterpene system, were isolated from endophytic sp. KMU18029. Their structures were elucidated on the basis of spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism calculations.