Phage-driven coevolution reveals trade-off between antibiotic and phage resistance in .

Phage therapy faces challenges against multidrug-resistant (MDR) due to rapid phage-resistant mutant emergence. Understanding the intricate interplay between antibiotics and phages is essential for shaping evolution and advancing phage therapy. In this study, MDR () 2089b coevolved with phage JNwz02 for 30 passages (60 days), then the effect of coevolution on the trade-off between phage and antibiotic resistance in bacteria was investigated.

Characterization of a broad-spectrum endolysin rLysJNwz and its utility against Salmonella in foods.

Salmonella is a common foodborne pathogen worldwide. The use of bacteriophage-encoded endolysins as antimicrobial agents is a promising approach for controlling pathogenic contamination. In this context, a recombinant endolysin named rLysJNwz, consisting of a single domain falling with the L-alanogyl-D-glutamate peptidase-like family, was cloned, expressed, and characterized.

The clinical significance and function of EGFR mutation in TKI treatments of NSCLC patients.

Background: EGFR mutations widely exists in NSCLC patients, which are involved in cancer development.

Objective: The function of EGFR mutations in the resistance to TKI treatments of NSCLC was evaluated to provide theoretical support for the clinical management of NSCLC patients.

Methods: A total of 150 NSCLC patients including 118 patients with EGFR mutation and 32 without, were included in this study.

A Web-Based Prediction Model for Cancer-Specific Survival of Elderly Patients With Hypopharyngeal Squamous Cell Carcinomas: A Population-Based Study.

Hypopharyngeal squamous cell carcinomas (HPSCC) is one of the causes of death in elderly patients, an accurate prediction of survival can effectively improve the prognosis of patients. However, there is no accurate assessment of the survival prognosis of elderly patients with HPSCC. The purpose of this study is to establish a nomogram to predict the cancer-specific survival (CSS) of elderly patients with HPSCC.

Optical feedback interferometry with 40 km transmission distance based on a distributed feedback fiber laser.

This Letter proposes an all-fiber self-mixing interferometric method based on laser feedback technology with long transmission distance, which has advantages of high sensitivity and compact structure. We theoretically and experimentally verify that the measurement distance of the self-mixing sensor is not limited to the coherence length of the solitary laser used. In the experiment, the velocity of a non-cooperative target was successfully measured with 40 km transmission distance.

Relaxation characteristics for efficient fluorescence lifetime detection of gain media in the fiber laser.

By using different active materials as gain media, we demonstrate a system aimed to measure the fluorescence lifetime of gain media in the fiber laser by utilizing the correlation between the laser's relaxation oscillation characteristics and the fluorescence lifetime of the active materials. As the advantages of extremely compact configuration, non-destructive and real time of the system, the fluorescence lifetime of gain media composed of materials of erbium-ytterbium-doped or erbium-doped have been measured, whose results are 20.2 µs and 1.

Significant Benefits of Afatinib and Apatinib in a Refractory Advanced NSCLC Patient Resistant to Osimertinib: A Case Report.

EGFR-tyrosine kinase inhibitors (TKIs) have revolutionized the treatment for NSCLC. However, acquired drug resistance often occurs after treatment with EGFR-TKIs. T790M and C797S mutations are the most common resistance mechanism in patients who failed from first- and third- generation EGFR TKI treatments, respectively.

Coherent laser detection of the femtowatt-level frequency-shifted optical feedback based on a DFB fiber laser.

The theoretical basis and experimental realization of an all-fiber self-mixing laser Doppler velocimetry based on frequency-shifted feedback in a distributed feedback (DFB) fiber laser are presented, which employs a pair of fiber-coupled acousto-optic modulators to adjust the modulation intensity and frequency of the laser self-mixing effect. Moreover, the minimum optical feedback intensity for the velocity signal successfully measured by the interferometer is 5.12 fW, corresponding to 0.

All-fiber laser feedback interferometer using a DBR fiber laser for effective sub-picometer displacement measurement.

A fiber autodyne laser feedback displacement sensor based on the effect of the frequency shift is demonstrated. The all-fiber structure enables our system to be easily employed in diverse complex and narrow scenes. By virtue of adopting an ultra-high sensitivity distributed Bragg reflector (DBR) fiber laser as the laser source and the frequency-shift technology to avoid the phenomenon that the measured signal of the low frequency is submerged in the noise, the measurement of the sub-picometer displacement under weak feedback condition has been achieved, which shows a great potential in the field of micro-vibration measurement.

All-fiber self-mixing laser Doppler velocimetry with much less than 0.1 pW optical feedback based on adjustable gain.

The all-fiber self-mixing laser Doppler velocimetry with adjustable gain is experimentally investigated based on a distributed Bragg reflector fiber laser. In the measurement system, the modulation gain of the injected light in the laser cavity is adjusted by a pair of fiber-coupled acousto-optic modulators (AOMs) in the external cavity, which can change the intensity and frequency of the self-mixing modulation effect. Simultaneously, the minimum feedback intensity from the target to the laser for successful measurements is 0.

Comparison of the Prognostic Value of Systemic Inflammation Response Markers in Small Cell Lung Cancer Patients.

Increasing evidences support that systemic inflammation-based prognostic scores, modified Glasgow Prognostic Score (mGPS), C-reactive Protein/Albumin (CRP/ALB), Albumin/Globulin (AGR), Prognostic Nutritional Index (PNI) and Advanced Lung cancer Inflammation index (ALI), are key determinants of patients' outcome in solid tumors. However, in small cell lung cancer (SCLC), there have been no direct comparisons of them. Thus, the aim of this study was to compare the prognostic value of these markers in SCLC, and select a most appropriative one.

Treatment of rats with Jiangzhi Capsule improves liquid fructose-induced fatty liver: modulation of hepatic expression of SREBP-1c and DGAT-2.

Background: Jiangzhi Capsule is an Australian listed patented traditional Chinese medicine and has been used for management of lipid abnormalities over the past 10 years. To obtain a better understanding regarding Jiangzhi Capsule, the present study investigated the effects and underlying mechanisms of Jiangzhi Capsule on chronic fructose overconsumption-induced lipid abnormalities.

Methods: Male rats were treated with liquid fructose in their drinking water over 14 weeks.