TOP2A Promotes Proliferation, Migration, and Inflammatory Response in M5-Treated Keratinocytes by Binding CTBP1 to Activate Wnt/β-Catenin Signaling.

Psoriasis is a chronic cutaneous disease, affecting a significant portion of the global population. Topoisomerase II alpha (TOP2A) is upregulated in psoriasis samples, but the precise molecular mechanism remains unclear. We aimed to clarify the biological contribution of TOP2A in psoriasis progression.

Lymphocyte inhibition is compromised in mesenchymal stem cells from psoriatic skin.

Background: Psoriasis is a chronic inflammatory skin disorder associated with a host of immune abnormalities. Mesenchymal stem cells (MSCs) have immunosuppressive properties and, in earlier studies, we found that the bone marrow MSCs of patients with psoriasis exhibit abnormal cytokine secretion. Since MSCs can be isolated from skin, we hypothesized that the biological characteristics of MSCs in psoriatic skin lesions might reflect the pathogenesis of psoriasis.

Abnormalities in cytokine secretion from mesenchymal stem cells in psoriatic skin lesions.

Background: Psoriasis is a chronic inflammatory and proliferative skin disease associated with immune abnormalities. In our preliminary studies, it was found that bone marrow mesenchymal stem cells (BMSCs) were abnormal in patients with psoriasis. Mesenchymal stem cells (MSCs) are not only present in the bone marrow, but can also be separated from the skin.

Differential gene expression in peripheral blood T cells from patients with psoriasis, lichen planus, and atopic dermatitis.

Background: Psoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.

Objective: We used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.

Methods: After peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.

DNA methylation of dermal MSCs in psoriasis: identification of epigenetically dysregulated genes.

Background: Mesenchymal stem cells (MSCs) are likely involved in pathological processes of immune-related diseases, including psoriasis, because of their immunoregulatory and pro-angiogenic effects. DNA methylation plays an essential role in regulating gene expression and maintaining cell function.

Objective: This study aimed to investigate the gene methylation profile of dermal MSCs from patients with psoriasis.

Narrowband ultraviolet B interferes with gene expression in the peripheral blood T cells of patients with psoriasis.

Background: Psoriasis pathogenesis and development are closely related to abnormal T cell activity. Narrowband ultraviolet B (NB-UVB) treatment markedly improves skin lesions in psoriasis.

Objectives: To investigate differential gene expression in psoriasis and to understand the possible mechanisms of NB-UVB therapy for psoriasis.