Functional Role of a Novel Long Noncoding RNA in Esophageal Squamous Cell Carcinoma Progression and Metastasis.

Emerging studies demonstrate that long noncoding RNAs (lncRNA) participate in the regulation of various cancers. In the current study, a novel has been identified and explored in esophageal squamous cell carcinoma (ESCC). To discover a new regulatory circuitry in which RNAs crosstalk with each other, the transcriptome of lncRNA-miRNA-mRNA from ESCC and adjacent nonmalignant specimens were analyzed using multiple microarrays and diverse bioinformatics platforms.

[Study on lignans of Polygonum capitatum].

Objective: To study the lignan of Polygonum capitatum.

Methods: The compounds were isolated and purified by using of SiO2, Sephadex LH-20 column chromatography and pre-HPLC. The structures of the compounds were determined on the basis of their physical properties and the spectral data.

Altered mRNA expressions of sialyltransferases in human gastric cancer tissues.

Aberrant sialylation and altered sialyltransferase (ST) expressions are frequently found in many types of cancer. In this study, we examined the expressions of α 2,3-linked sialic acid residues and STs mRNA in human gastric cancer tissues. The relationship between the levels of α 2,3-linked sialic acid residues and the expression of STs mRNA was also analyzed.

Cardioprotective effect of 'methylamine irisolidone', a new compound, in hypoxia/reoxygenation injury in cultured rat cardiac myocytes.

'Methylamine irisolidone' (=5,7-dihydroxy-6-methoxy-3-(4-methoxyphenyl)-8-[(methylamino)methyl]-4H-[1]benzopyran-4-one), a new compound, is a structurally modified kakkalide with good water solubility. In this study, we investigated its effect on hypoxia/reoxygenation (H/R) injury in cultured rat cardiac myocytes. The results showed that methylamine irisolidone could significantly inhibit lactate dehydrogenase (LDH) release, enhance the mitochondrial membrane potential, decrease intracellular calcium (Ca(2+)) associated with the attenuation of reactive oxygen species (ROS) generation, reduce contents of malondialdehyde (MDA), and increase the activity of superoxide dismutase (SOD) after H/R in a dose-dependent manner.

Protective effects of Salvia plebeia compound homoplantaginin on hepatocyte injury.

Salvia plebeia R. Br is a traditional Chinese herb which has been considered as an inflammatory mediator used for treatment of many infectious diseases including hepatitis. Previously, the compound homoplantaginin was isolated in our group.

[Study on C21 steroidal glycosides of Cynanchum atratum by LC-MS].

High performance liquid chromatography coupled with on-line electrospray tandem mass spectrometry (HPLC/ESI-MS/MS) was used to identify C21 steroidal glycosides in the roots of Cynanchum atratum. The structures of C21 steroidal glycosides were deduced from mass fragments features in positive and negative mode. The constituents of C.

Twelve pregnane glycosides from Cynanchum atratum.

Eleven new 14,15-seco-pregnane-type steroidal glycosides, cynanosides P1-P5, Q1-Q3, R1-R3, and a novel 12,13-seco-14,18-nor-pregnane-type steroidal glycoside, cynanoside S, were isolated from the roots of Cynanchum atratum, together with four known compounds, atratoside C, sublanceoside E3, chekiangensoside C and cynatroside B. Their structures were determined on the basis of spectroscopic analysis and chemical evidence.

A new homoisoflavan from Caesalpinia sappan.

A new homoisoflavan, 7,3',4'-trihydroxy-3-benzyl-2H-chromene (1), was isolated from the dried heartwood of Caesalpinia sappan L., together with seven known phenolic compounds. The structure of the new compound (1) was determined on the basis of spectroscopic analysis.

A major triterpenoid saponin from Gypsophila oldhamiana.

A new saponin, gypoldoside A (1), was isolated from the roots of Gypsophila oldhamiana. On the basis of in-depth NMR-spectroscopic and mass-spectrometric analysis, in combination with chemical evidence, its structure was established as 3-O-{beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-beta-D-glucuronopyranosyl}quillaic acid 28-[alpha-L-arabinopyranosyl-(1-->2)-alpha-L-arabinopyranosyl-(1-->3)-beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-fucopyranosyl] ester. Compound 1 was found to be highly active against three different human cancer cell lines, with IC50 values in the low micromolar range.