Detecting epithelial-mesenchymal transition signaling molecules in cervical epithelial cells aids in the early diagnosis of cervical lesions.

Background: This study aimed to investigate the potential utility of Epithelial-mesenchymal transition (EMT) signaling cell detection in the early diagnosis of cervical lesions.

Methods: Enrichment of cervical epithelial cells was carried out using a calibrated membrane with 8-μm diameter pores. RNA-in situ hybridization (RNA-ISH) was employed to detect and characterize EMT cells utilizing specific EMT markers.

User-Perceived Capacity: Theory, Computation, and Achievable Policies.

User-perceived throughput is a novel performance metric attracting a considerable amount of recent attention because it characterizes the quality of the experience in mobile multimedia services. For instance, it gives a data rate of video streaming with which a user will not experience any lag or outage in watching video clips. However, its performance limit remains open.

Significance of circulating tumor cells detection in tumor diagnosis and monitoring.

To detect circulating tumor cells (CTCs) in the peripheral blood of patients with tumor, and to analyze the significance of CTC detection in tumor diagnosis and monitoring. In the present study, peripheral blood was collected from 125 patients with tumor, and CTCs were isolated and identified. Differences in CTC number and subtype detection were analyzed for different tumor diseases and stages.

Star Polymer-Based Nanodelivery System for Pesticides: Enhanced Broad-Spectrum Toxicity and Selective Toxicity.

The application of nanotechnology in agriculture can break through many traditional problems of synthetic pesticides, especially for increasing bioactivity and reducing application amount. However, the safety and selective toxicity of nanocarrier-loaded pesticides should be clarified toward natural predators. In this context, an efficient spirotetramat nanodelivery system was successfully constructed based on a star polymer (SPc).

The Effect of Antibiotics Treatment on the Maternal Immune Response and Gut Microbiome in Pregnant and Non-Pregnant Mice.

The gut microbiota are involved in adaptations of the maternal immune response to pregnancy. We therefore hypothesized that inducing gut dysbiosis during pregnancy alters the maternal immune response. Thus, pregnant mice received antibiotics from day 9 to day 16 to disturb the maternal gut microbiome.

Identification of Distinct Immune Cell Subsets Associated With Asymptomatic Infection, Disease Severity, and Viral Persistence in COVID-19 Patients.

The cell-mediated protective and pathogenic immune responses to SARS-CoV-2 infection remain largely elusive. Here we identified 76 distinct cell subsets in the PBMC samples that were associated with various clinical presentations of COVID-19 using scRNA-seq technology coupled with a deep and comprehensive analysis of unique cell surface markers and differentially expressed genes. We revealed that (TRAV1-2CD8)MAIT cells and (NCAM1CD160)NK cells significantly enriched in the asymptomatic subjects whereas (LAG3CD160CD8)NKT cells increased in the symptomatic patients.

Selective Chemical Labeling and Sequencing of 5-Hydroxymethylcytosine in DNA at Single-Base Resolution.

5-Hydroxymethylcytosine (5hmC), the oxidative product of 5-methylcytosine (5mC) catalyzed by ten-eleven translocation enzymes, plays an important role in many biological processes as an epigenetic mediator. Prior studies have shown that 5hmC can be selectively labeled with chemically modified glucose moieties and enriched using click chemistry with biotin affinity approaches. Besides, DNA deaminases of the AID/APOBEC family can discriminate modified 5hmC bases from cytosine (C) or 5mC.

Microbiota Induced Changes in the Immune Response in Pregnant Mice.

Pregnancy is associated with adaptations of the immune response and with changes in the gutmicrobiota. We hypothesized the gut microbiota are involved in inducing (part of) the immunological adaptations during pregnancy. To test this hypothesis, we collected feces from pregnant conventional mice before and during pregnancy (days 7, 14, and 18) and microbiota were measured using 16S RNA sequencing.

Integrated transcriptome, proteome and physiology analysis of Epinephelus coioides after exposure to copper nanoparticles or copper sulfate.

Copper nanoparticles (Cu-NPs) are components in numerous commercial products, but little is known about the mechanisms of their toxicity to marine fish. Here, we investigated physiology, transcriptome and proteome in Epinephelus coioides after exposure to Cu as Cu-NPs or copper sulfate (CuSO). Aggregation, oxidation and dissolution of Cu-NPs occurred after suspension in seawater within 24 h.

Transcriptome Analysis of Liangshan Pig Muscle Development at the Growth Curve Inflection Point and Asymptotic Stages Using Digital Gene Expression Profiling.

Animal growth curves can provide essential information for animal breeders to optimize feeding and management strategies. However, the genetic mechanism underlying the phenotypic differentiation between the inflection point and asymptotic stages of the growth curve is not well characterized. Here, we employed Liangshan pigs in stages of growth at the inflection point (under inflection point: UIP) and the two asymptotic stages (before the inflection point: BIP, after the inflection point: AIP) as models to survey global gene expression in the longissimus dorsi muscle using digital gene expression (DGE) tag profiling.