Publications by authors named "Yuanlong Shuai"
The telomerase complex consists of a protein component (TERT), which has reverse transcriptase activity, and an RNA component (TERC), which serves as a template for telomere synthesis. Evidence is rapidly accumulating regarding the non-canonical functions of these components in both normal or diseased cells. An oligonucleotide-based drug, the first telomerase inhibitor, secured FDA approval in June 2024.
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- CRISPRmap is a new method for optical pooled genetic screening that allows researchers to study spatial phenotypes without destroying cells, focusing on how cells respond to CRISPR modifications.
- This method integrates guide-identifying barcodes with advanced imaging techniques like immunofluorescence and RNA detection to improve the efficiency of reading the genetic information.
- In a study using breast cancer cells, CRISPRmap helped identify potentially harmful gene mutations associated with DNA damage repair, providing insights into their impact on treatment responses.
Pooled genetic screens are powerful tools to study gene function in a high-throughput manner. Typically, sequencing-based screens require cell lysis, which limits the examination of critical phenotypes such as cell morphology, protein subcellular localization, and cell-cell/tissue interactions. In contrast, emerging optical pooled screening methods enable the investigation of these spatial phenotypes in response to targeted CRISPR perturbations.
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