Effects of acrylonitrile on apoptosis of rat cerebral nerve cells.

The brain, especially the hippocampus, is sensitive to damage caused by anoxic chemicals. In this study, we established a rat model of acrylonitrile poisoning with administration by gavage, aiming to determine the influence of acrylonitrile on rat cerebral nerve cells. Transmission electron microscopy observation and TdT-mediated dUTP nick-end labelling (TUNEL) staining were used to explore preliminarily the apoptotic changes of cerebral nerve cells.

The value of APACHE II in predicting mortality after paraquat poisoning in Chinese and Korean population: A systematic review and meta-analysis.

Background: The Acute Physiology and Chronic Health Evaluation II (APACHE II) score is used to determine disease severity and predict outcomes in critically ill patients. However, the prognostic significance of APACHE after acute paraquat (PQ) poisoning remains unclear. The meta-analysis was aimed to study the value of APACHE II in predicting mortality in PQ-exposed Chinese and Korean patients.

The Cytotoxic Effect of the Benzene Metabolite Hydroquinone is Mediated by the Modulation of MDR1 Expression via the NF-κB Signaling Pathway.

Background/aims: Benzene is a toxic chemical whose leukemogenic effects have been studied for decades. The mechanisms of benzene-induced toxicity and leukemogenicity are not fully understood, although the involvement of several pathways has been suggested, including oxidative stress, DNA damage, cell cycle regulation and programmed cell death. In the present study, we investigated the effect of hydroquinone (HQ), a major benzene metabolite, on the viability of bone marrow derived mesenchymal stem cells (BMSCs) and explored the underlying mechanisms.

Effects of low concentrations of benzene exposure on levels of platelet-associated antibodies and platelet parameters.

Objective: To evaluate the impact of exposure to low concentrations of benzene on the platelet-associated antibodies and platelet parameters.

Methods: We carried out an analysis on 121 benzene-exposed workers and 110 healthy workers whose blood samples were collected and the levels of platelet-associated antibodies and platelet parameters were assessed. Benzene emissions were monitored over 5 years.

[The effects of acrylonitrile on T lymphocyte subsets and expression of toll-like receptor 4 in rats].

Objective: To explore the effects of acrylonitrile on T lymphocyte subsets, expression of toll-like receptor 4 and related cytokines in rats.

Methods: Sixty-four Sprague-Dawley rats were randomly divided into 4 female groups and 4 male groups, and there were 8 rats in each group. Rats in each group were respectively given a single dose of 0, 5, 10 and 20 mg/kg acrylonitrile by gavage, once a day, 5 days a week, for 13 weeks.

[Occupational acute dimethylformamide poisoning: an analysis of 16 cases].

Objective: To analyze the clinical features and diagnostic points of occupational acute dimethylformamide (DMF) poisoning and to explore the mechanism of occupational acute DMF poisoning.

Methods: A comprehensive analysis was performed on the clinical data of 16 cases of occupational acute DMF poisoning, including symptoms, signs, and laboratory testing results.

Results: The main clinical features of occupational acute DMF poisoning were digestive system impairments, especially abdominalgia.

[The effects of benzene poisoning on expression of multidrug resistance 1 gene and P-glycoprotein in bone marrow mononuclear cells of C57BL/6 mice].

Objective: To investigate the effects of benzene poisoning on the expression of multidrug resistance 1 (MDR1) gene and P-glycoprotein (P-gp) in the bone marrow mononuclear cells (BMMNCs) of C57BL/6 mice.

Methods: C57BL/6 mice were randomly divided into control group (n = 24), low-dose group (n = 24), medium-dose group (n = 24), and high-dose group (n = 24) to receive corn oil, 25 mg/kg benzene, 50 mg/kg benzene, or 100 mg/kg benzene by gavage, once daily, 5 days/weeks, for 4 weeks. The mice were sacrificed on day 12, 26, or 29 of poisoning.

[High-resolution melting: a analysis for genotyping of MDR1 C3435T in benzene-exposed workers].

Objective: Using high resolution melting (HRM) to analysis MDR1 C3435T in people exposed to benzene.

Methods: Restriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them.

Results: By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got.

[Influence of MDR1 gene C3435T on peripheral white blood cell counts in workers exposed to benzene].

Objective: To explore the effects of MDR1 C3435T on the peripheral white blood cell counts in workers exposed to benzene.

Methods: One hundred and twenty-one benzene-exposed workers and 110 healthy controls without benzene exposure were enrolled in this study. White blood cell counts influenced by the polymorphism of MDR1 gene were analyzed.

[Application of micronucleus test of buccal mucosal cells in assessing the genetic damage of workers exposed to acrylonitrile].

Objective: To investigate the application of micronucleus test of buccal mucosal cells in monitoring the genetic effect of acrylonitrile in the population exposed to the acrylonitrile.

Methods: Forty-one healthy male workers in a chemical factory in Shanghai were selected as the low concentration acrylonitrile exposed group while forty-seven healthy male workers in an acrylonitrile factory in Shanghai were selected as the intermediate concentration acrylonitrile exposed group. At the same time, thirty-one male workers who had no toxicant exposure and lived in the same community were selected as the control group.

[Dynamic alterations of rat metabolic enzymes activities and liver damage in rat exposed to vinyl chloride monomer].

Objective: Study the alterations of metabolic enzyme activities and liver functions induced by vinyl chloride monomer(VCM) and to explore the relationship between metabolic enzyme activities and liver functions.

Methods: Animals were administered VCM for 12 weeks in doses of 0, 5, 10, 20 mg/kg (i.p.

[Study on mitochondrial DNA damage in peripheral blood nucleate cells of the workers exposed to acrylonitrile].

Objective: To study the potential aging effect on workers exposed to acrylonitrile (ACN).

Methods: The deletion rates of mitochondrial DNA (mtDNA) in peripheral blood nucleate cells of 47 exposed workers and 47 non-exposed workers (as control), as well as 12 old people and 12 young people were measured with polymerase chain reaction (PCR).

Results: The positive rates of mtDNA deletion in peripheral blood nucleate cells were 17.