Publications by authors named "Yuanli Gao"

Synthetic genetic circuits program the cellular input-output relationships to execute customized functions. However, efforts to scale up these circuits have been hampered by the limited number of reliable regulatory mechanisms with high programmability, performance, predictability and orthogonality. Here we report a class of split-intron-enabled trans-splicing riboregulators (SENTRs) based on de novo designed external guide sequences.

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  • A study evaluated a new long-lasting pain relief medication called QP001, compared to a placebo, to determine its effectiveness and safety for patients after abdominal surgery.
  • Conducted across 23 centers, 255 patients were administered either QP001 or placebo, and the results showed QP001 significantly reduced pain intensity for up to 24 hours post-surgery.
  • The trial indicated that QP001 also reduced the need for morphine and increased patient satisfaction, with no significant difference in adverse events compared to placebo.
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  • This study investigated the effectiveness and safety of a new pain medication, QP001, for managing postoperative pain in patients after orthopaedic surgery in China.
  • In a clinical trial with 198 participants, those taking QP001 consumed significantly less morphine for pain relief compared to those receiving a placebo, both at 24 hours and 48 hours post-surgery.
  • QP001 not only reduced the need for opioids but also had fewer adverse drug reactions, indicating it could be a promising alternative for pain management after surgery.
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RNA sensing in vivo evaluates past or ongoing endogenous RNA disturbances, which is crucial for identifying cell types and states and diagnosing diseases. Recently, the CRISPR-driven genetic circuits have offered promising solutions to burgeoning challenges in RNA sensing. This review delves into the cutting-edge developments of CRISPR-powered RNA sensors in vivo, reclassifying these RNA sensors into four categories based on their working mechanisms, including programmable reassembly of split single-guide RNA (sgRNA), RNA-triggered RNA processing and protein cleavage, miRNA-triggered RNA interference (RNAi), and strand displacement reactions.

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Synthetic biology aims to engineer biological systems for customized tasks through the bottom-up assembly of fundamental building blocks, which requires high-quality libraries of reliable, modular, and standardized genetic parts. To establish sets of parts that work well together, synthetic biologists created standardized part libraries in which every component is analyzed in the same metrics and context. Here we present a state-of-the-art review of the currently available part libraries for designing biocircuits and their gene expression regulation paradigms at transcriptional, translational, and post-translational levels in .

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Parasite-specific CD4 Th1 cell responses are the predominant immune effector for controlling malaria infection; however, the underlying regulatory mechanisms remain largely unknown. This study demonstrated that ATG5 deficiency in myeloid cells can significantly inhibit the growth of rodent blood-stage malarial parasites by selectively enhancing parasite-specific CD4 Th1 cell responses. This effect was independent of ATG5-mediated canonical and non-canonical autophagy.

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As synthetic biology permeates society, the signal processing circuits in engineered living systems must be customized to meet practical demands. Towards this mission, novel regulatory mechanisms and genetic circuits with unprecedented complexity have been implemented over the past decade. These regulatory mechanisms, such as transcription and translation control, could be integrated into hybrid circuits termed "multi-level circuits".

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Temperature affects the growth and yield of yam ( Thunb.), and calcium-dependent protein kinases (CDPKs) play an important role in the plant stress response. However, there has been a lack of system analyses of yam's gene family.

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  • A new medicine called QP001 was tested to help manage pain after abdominal surgery.
  • The study involved patients who either got QP001 or a fake medicine (placebo) before their surgery to see how much pain relief they had.
  • Results showed that patients who received QP001 needed less morphine for pain compared to those who got the placebo, and there were no big safety concerns.
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Malaria infections are persistent as frequent recrudescence of the disease may occur following the acute infection stage, but the different immune responses that control the acute and recrudescence stages are still largely unknown. Using single-cell RNA sequencing (scRNA-seq), we showed that the number of Th1 and plasma cells in the spleen was significantly reduced during the recurrence stage compared to the acute stage of () infection. Additionally, the ability of both CD4 T cell responses and B cells to control recurrence was significantly reduced compared to their roles in the control of acute infection.

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BACKGROUND Showing an informational anesthesia video can reduce the preoperative anxiety of parturients undergoing elective cesarean section (CS). However, the best method for presenting such videos remains unclear, and whether such videos can reduce the anxiety level of women during the entire perioperative period for CS (including preoperative and postoperative) has not been studied yet. MATERIAL AND METHODS This study was a single-center prospective randomized trial.

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A sol-gel technique was applied for the preparation of water-compatible molecularly imprinted polymer (MIP) for solid phase microextraction (SPME) using diazinon as template and polyethylene glycol as functional monomer. The MIP-coated fiber demonstrated much better selectivity to diazinon and its structural analogs in aqueous cucumber sample than in distilled water, indicating its potential in real samples. Thanks to its specific adsorption as well as rough and porous surface, the coating revealed rather larger extraction capability than the non-imprinted polymer and commercial fibers.

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Multiple headspace solid-phase microextraction (HS-SPME) using a novel fiber coated with anilino-methyl triethoxy silicane-methacrylic acid/terminated silicone oil has been introduced as a useful pretreatment technique coupled to gas chromatography-flame ionization detector for the detection of ethyl carbamate in pickles. Anilino-methyl triethoxy silicane and methacrylic acid are put into use simultaneously with the aim to increase the hydrogen interaction strength between ethyl carbamate and the coating. In addition, the new fiber exhibits high thermal stability, good reproducibility, and long lifetime.

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This study presents the potential of multiple headspace solid-phase microextraction (multiple HS-SPME) for the quantification of analytes in solid samples. Multiple HS-SPME shares the same advantages as SPME. It also enables a complete recovery of the target compound and therefore the matrix effect, which commonly appears in SPME-based analysis, is avoided.

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Lipopolysaccharide (LPS)-induced inflammatory factors production by the cerebral cortical glial cells in two sides of the murine brain are different. To determine if microglial cells, a subset of glial cells, are involved in asymmetric production, interleukin-6 (IL-6), interleukin-1β (IL-1β) and nitric oxide (NO) responses to LPS by microglial cells in the right and left cerebral cortices were examined. Primary microglial cells were isolated from BALB/C neonatal mice, treated with LPS (10 µg ml(-1) ) for 24 h and examined for IL-6, IL-1β and NO production.

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Background & Objectives: The distribution of brain interleukin-6 (IL-6) may be asymmetrical both in cortex and hippocampus. While the brain asymmetry has been extensively investigated, the cellular origin of asymmetrical cytokine induction in the cortex has not been addressed. It was hypothesized that the immune function of glia cell to the inflammatory insults is asymmetrically distributed in the two brain hemispheres.

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Patients with an influenza virus infection can be complicated by acute encephalopathy and encephalitis. To investigate the immune reactions involved in the neurocomplication, mouse microglia and astrocytes were isolated, infected with human H1N1 and avian H5N1 influenza viruses, and examined for their immune responses. We observed homogeneously distributed viral receptors, sialic acid (SA)-alpha2,3-Galactose (Gal) and SA-alpha2,6-Gal, on microglia and astrocytes.

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