Generation of a human iPSC line CIPi004-A from a patient with neurofibromatosis type 1 and epilepsy harboring a heterozygous mutation in NF1 gene.

The NF1 gene is related to neurofibromatosis type 1 (NF1), which is an autosomal dominant disorder associated with multisystem involvement and epilepsy susceptibility. A human induced pluripotent stem cell (iPSC) line was derived from a pediatric patient with NF1 and epilepsy, harboring a heterozygous NF1 gene mutation. The iPSC line exhibits high levels of pluripotency markers, maintains the NF1 gene mutation, and demonstrates the capacity to undergo differentiation potential in vitro into three germ layers.

Down-expression pattern of Ku70 and p53 coexisted in colorectal cancer.

To address the relationship of altered expression of double-strand break repair proteins Ku70 and p53 in clinical colorectal cancer (CRC), we examined the expression pattern of Ku70 and p53 by using fluorescent immunohistochemistry and real-time PCR assays in CRC and pericancerous samples from 152 Chinese patients. The results showed that down-expression pattern of both Ku70 and p53 coexisted in the CRC samples with significant correlating rate (R (2) = 0.9103; P < 0.

Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients.

Background: Double-strand DNA breaks (DSBs) are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM) in colorectal cancer (CRC), we examined the expression levels and patterns of Ku70 and ATM in CRC samples.

TYMS serves as a prognostic indicator to predict the lymph node metastasis in Chinese patients with colorectal cancer.

Objective: The current study is to evaluate the effect of thymidylate synthase (TYMS) on lymph node metastasis (LNM) in Chinese colorectal cancer (CRC) patients, and develop potential LNM-associated biomarkers for CRC.

Design And Methods: Differences in TYMS gene expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed using quantitative real-time PCR analysis in 100 Chinese colorectal cancer patients. The relationship between clinicopathological parameters and prognosis of candidate biomarkers was also examined in the experiment.