Publications by authors named "Yuan-xiang Chen"
[Prokaryotic expression of HPV 16L1 and optimization of expression conditions].
Zhejiang Da Xue Xue Bao Yi Xue Ban
July 2010
Objective: To construct the HPV16 L1 prokaryotic expression plasmid and to optimize its expression.
Methods: A pair of primers was designed according to plasmid sequences of pGEX-KG and the HPV16L1 genes published by GeneBank. The DNA fragment of 1500 bp was amplified by PCR from the HPV recombinant plasmid with HPV16L1 gene, then cloned into pGEX-KG and transformed into the host E.