[Validation of candidate immunogenic membrane antigens of human pancreatic cancer screened by proteomics].

Objective: To validate those obtained immunogenic membrane antigens candidate of human pancreatic cancer in the performed research.

Methods: In the pre-studies, serum IgG purified from clinically collected sera of pancreatic cancer patients underwent immunoblot with human pancreatic cancer cell line SW1990 membrane protein, totally obtained 9 positive protein spots. Number 5 and 6 positive dots of immunoblot were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and peptide mass fingerprinting matching.

[Candidate immunogenic membrane antigens of human pancreatic cancer].

Objective: To verify the obtained immunogenic membrane antigens candidate of pancreatic cancer in the performed research.

Methods: Pancreatic cancer cell line SW1990 membrane protein underwent immunoblot with serum IgG purified from clinically collected sera of 66 pancreatic cancer patients. Number 3 and number 8 positive dots of immunoblot were identified by MALDI-TOF mass spectrometry and peptide mass fingerprinting matching.

[Research of immunogenic membrane antigens of pancreatic cancer].

Objective: To screen and obtain the validate immunogenic membrane antigens in pancreatic cancer.

Methods: Pancreatic cancer cell line SW1990 membrane protein was extracted and separated by two-dimensional gel electrophoresis (2-DE). One of the three parallel 2-DE gels underwent Coomassie blue staining while the other two underwent immunoblot.

[Selection of genes related to multidrug resistance of pancreatic ductal adenocarcinoma by microarray analysis].

Objective: To investigate the genes concerning multidrug resistance (MDR) of pancreatic ductal adenocarcinoma with microarray analysis.

Methods: Gene expression profile of pancreatic cancer cell line SW1990 and resistance subline SW1990/5-FU, SW1990/ADM, SW1990/GEM were screened in two independent replicates using oligonucleotide microarray (Affymetrix HG U133 2.0 plus) which contained 38,500 human genes.

Microarray analysis of gene expression profile of multidrug resistance in pancreatic cancer.

Background: Chemotherapy is the most frequently adopted adjuvant therapy of pancreatic ductal adenocarcinoma (PDAC), but the development of drug resistance reduces its effectiveness. Clarification of the mechanism of multidrug resistance (MDR) development in PDAC is needed to improve the therapeutic effect of chemotherapy. This study was aimed to investigate the molecular mechanism of MDR of PDAC and to identify genes associated with MDR development.

[The relationship between the changes of proangiogenic factors serum concentrations and progression of pancreatic carcinoma patients].

Objective: To investigate the relationship between VEGF, bFGF and IGF-1 serum concentration and progression of pancreatic carcinoma.

Methods: Fifty-six patients with pancreatic carcinoma were divided into resectable group (n = 32) and unresectable group (n = 24). Another group was normal group (n = 20).

[The method of inducing and establishing human pancreatic cancer cell sublines with radiation resistance].

Objective: To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance.

Methods: Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1 (Cap), AsPC-1 (ASPC), P3, PANC-1 (Pan-1) and MIAPaCa-2 (MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.

Results: Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation.

[Expression and significance of GCS gene in human pancreatic cancer cell SW1990 and its drug-resistant sublines].

Objective: To study the expression and significance of GCS gene in human pancreatic cancer cell line SW1990 and its drug-resistant sublines.

Methods: SW1990 and its drug-resistant sublines, SW1990/FU, SW1990/ADM and SW1990/GEM were cultured in vitro. CCK-8 (Cell Counting kit-8) was used to detect the drug resistance of the sublines.

[Relationship between apoptosis induced by 2-butylamino-2-demethoxy-hypocrellin B in human pancreatic cancer cells Capan-1 and photosensitization of mitochondria].

Objective: To explore the possible mechanism of apoptosis induced by photodynamic therapy (PDT) in human pancreatic cancer cells Capan-1 with 2-butylamino-2-demethoxy-hypocrellin B (BAHB) as photosensitizer.

Methods: The localization of BAHB in Capan-1 cells was studied, apoptosis was determined by DNA gel electrophoresis after PDT. The mitochondria membrane potential (DYm) and cytochrome C release were observed by laser scan confocal microscopy and Western blotting.

[Drug resistance and activity changes of thioredoxin reductase in pancreatic cancer cells strain SW1990 induced by gemcitabine].

Objective: To establish gemcitabine-resistant pancreatic cancer cell strain and study the role of thioredoxin reductase (TrxR) in drug-resistant process.

Methods: Gemcitabine-resistant pancreatic cancer cell strain SW1990/GZ was induced by increasing drug dosage intermittently, then the changes of its biological features and the activity of TrxR were examined.

Results: Stable drug-resistant SW1990/GZ cell strain was established by culturing with gemcitabine for 9 months.

[Effects of hydrogen peroxide on intracellular free Ca2+ content in rat liver oval cells].

Objective: To study the effects and mechanism of hydrogen peroxide (H2O2) of low concentration on dynamic changes of intracellular free calcium contents ([Ca2+]i) in cultural rat liver oval cells (WB-F344 cells).

Methods: Using Fluo-3/Am as fluorescent indicator of [Ca2+]i and it was measured by laser scanning confocal microscope system.

Results: The results showed that: (1) A rapid transient spiking of [Ca2+]i occurred after the stimulation of H2O2 of low concentration (800 nmol/L).