[Efficacy of subgingival scaling of the molars with conventional scalers in vitro].

Purpose: To evaluate the efficacy of traditional manual scaling and piezoelectric and magnetostrictive ultrasonic scaling in regard to periodontal pocket depth and power setting.

Methods: Thirty extracted human molars were assigned to receive manual scaling or ultrasonic scaling (both on the full or half power setting) with probing depth of 5 and 7 mm for 2 min. The remained oil materials on the roots were calculated as supplementary data.

[Comparison of subgingival debridement efficacy of air polishing and manual scaling].

Purpose: To assess the efficacy of subgingival air polishing and traditional manual scaling in 3-6 mm pockets.

Methods: Patients with chronic periodontitis who were in the maintenance phase were randomly assigned to receive subgingival air polishing (experimental group) and manual scaling (control group) in 4 teeth with probing depths of 3 to 6 mm. Clinical variables including plaque index (PI), probing depth (PD), probing on bleeding (BOP) and gingival recession (GR) were recorded at baseline, 7 and 30 days post-treatment.

[The effect of vascular endothelial cells on the migration of periodontal ligament cells and gingival fibroblasts].

Purpose: To investigate the effect of human vascular endothelial cells (HUVEC) on the migration of human periodontal ligament cells (HPDLC) and human gingival fibroblasts (HGF) through a model mimicking periodontal wound healing or regeneration.

Methods: HUVEC, HPDLC and HGF were co-cultured using a Transwell system, while HPDLC or HGF was cultured independently as control. Chemotaxis assay was performed using cell culture inserts (0.

[The reliability of the salivary occult blood to test gingival inflammation].

Purpose: To evaluate the reliability of salivary occult blood test(SOBT) for gingival inflammation.

Methods: Forty-five pre- and post-stimulation saliva samples were tested for occult blood, with bleeding on probing (BOP) as an indicator of gingival inflammation. Subjects with BOP%<15% were defined as control group.

[The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts].

Purpose: To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration.

Methods: Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control.

[Expression of IL-6 in cyclosporin A-induced gingival overgrowth].

Purpose: To estimate the role of IL-6 in cyclosporin A(CsA)-induced gingival overgrowth(GO) and collect the evidence of pathomechanism for CsA-induced GO.

Methods: Gingival fibroblasts and epithelial cells were treated with CsA of three different concentrations (600ng/mL, 800ng/mL, and 1000ng/mL) with different time (48h, 72h). After cell stretched preparation, the secretion of IL-6 was analyzed by ELISA while expression of IL-6 was analyzed by immunohistochemistry (ABC).

[Expression of TGF-β1 and PCNA in cyclosporin A-induced gingival overgrowth].

Purpose: To investigate the expression of transforming growth factor-β1(TGF-β1) and proliferating cell nuclear of antigen (PCNA) in cyclosporin A(CsA)-induced gingival overgrowth (GO) tissues.

Methods: After extraction of half of the maxillary molars, 20 male Sprague-Dawley rats were randomized into a CsA-injection group (10mg·kg⁻·d⁻¹) or a normal saline control group. Ten rats per group were sacrificed at the fourth week after injection.

[Evaluation of the remaining periodontal ligament from attachment level].

Purpose: To estimate the total periodontal ligament areas and the remaining periodontal ligament areas from different attachment level of each tooth type, and to determine the relationship between the attachment loss and the prognosis of periodontal diseases.

Methods: Four hundred and twenty extracted teeth (30 of each tooth type of one side of both arches,except for the third molar) were collected.The root surface of each tooth was coated with polyvinyl alcohol to produce a membrane after cleaning.

[The effect of dental plaque in cyclosporin A-induced gingival overgrowth].

Purpose: To evaluate the role of dental plaque in cyclosporin A(CsA)-induced gingival overgrowth.

Methods: After extraction of half of the maxillary molars, twenty male Sprague-Dawley rats were assigned to a CsA-injection group (10mg/k x d) or a control group. Ten rats per group were sacrificed at week 4.

[The effect of initial periodontal therapy on metabolic control in type 2 diabetes mellitus].

Purpose: To investigate the effect of periodontal initial therapy on metabolic control in type 2 diabetes mellitus patients with chronic periodontitis.

Methods: Thirty type 2 diabetic patients with chronic periodontitis were selected as experimental group, 30 patients with chronic periodontitis were selected as control group. Their gingival index(GI), probing depth(PD), clinical attachment level(CAL), fasting plasma glucose(FPG), glycated hemoglobin A1c(HbA1c), total cholesterol(TC) and triglyceride(TG) were evaluated using SAS 6.

[Periodontal wound filling affected by aging in an in vitro model].

Purpose: Using human periodontal ligament cells (PDLC) and gingival fibroblasts (GF) , that were cultured in vitro for a long period of time to simulate the aging cells, we evaluated the influence of aging on periodontal healing in an in vitro wound model.

Methods: Human PDLC and GF were cultured in vitro over to 12 passages as aging cells for experiment, the same type of cells that were cultured under 5 passages as younger for control. The in vitro wound models were mechanically created in subconfluent cultures of this 4 types of cells, by removing a 7 mm wide band of the cell layer respectively.

[Effect of enamel matrix protein on periodontal cells in an in vitro wound healing model].

Purpose: The aim of this study was to evaluate the influence of enamel matrix protein (EMP) on wound filling of periodontal ligament cells (PDLC) and gingival fibroblasts (GF), using an in vitro model of wound healing.

Methods: In vitro wound models were mechanically created in subconfluent cultures of human GF and PDLC, by removing a 7mm wide band of the cell layer respectively. Wounded cultures were then incubated for a time periods up to 2,6,9 days in a media containing 10% fetal bovine serum (FBS), stimulated with EMP (100microg/ml) simultaneously, negative controls were those cultured only with media containing 10% FBS.

[A study on the state of periodontal health of 400 professional adults by random sampling in Shanghai].

Purpose: To investigate and analyze the state of periodontal health of 400 adults at their post and risk factors in Shanghai.

Methods: 400 subjects (249 male, 151 female), aged from 25 to 59, were requested to fill a questionnaire. The probing depth (PD), clinical attachment level (CAL), tooth mobility (TM) and bleeding on probing(BOP) on 6 sites of each indicated tooth were measured and recorded.

[Histological evaluation of domestic poly-DL-lactic acid membranes for periodontal guided tissue regeneration].

Objective: To evaluate the possibility of domestic poly-DL-lactic acid membranes as barriers during periodontal guided tissue regeneration.

Methods: Two-wall intrabony defects were made by artificially removing buccal and distal alveolar bone from the distal roots of the second, third and fourth mandibular premolars as well as the first mandibular molars of dogs. These experiment defects received four membranes (PDLLA, ePTFE, COL, Vicryl ) respectively for GTR, the control treated without membrane.