NHC-BH-CH: synthesis, characterization and electrochemical properties of the N-heterocyclic-carbene-stabilized fullerene-borane complex.

NHC-[60]fullereborane (NHC-BH-CH) has been synthesized, isolated in pure form, and characterized. The reaction of C with NHC-borane produced NHC-BH-CH as the first air-stable and separatable fullerene-borane compound. The formation of a N-heterocyclic carbene stabilized this borane complex of C, and the structure of the complex was unambiguously determined throughsingle-crystal X-ray analysis.

Quadrivalent influenza vaccine (Sinovac Biotech) for seasonal influenza prophylaxis.

Introduction: Quadrivalent Influenza Vaccine (Sinovac Biotech) is a quadrivalent split-virion-inactivated influenza vaccine approved in China in June 2020 for individuals ≥3 years of age. It contains 15 µg hemagglutinin per strain including A/H1N1, A/H3N2, B/Victoria, and B/Yamagata, which could potentially improve protection against influenza B viruses.

Areas Covered: In this review, we summarize the development of quadrivalent influenza vaccines in China and foreign countries, and assess the immunogenicity and safety from the phase I and III clinical trials of Quadrivalent Influenza Vaccine in individuals ≥3 years of age.

A comparative analysis of immunogenicity and safety of an enterovirus 71 vaccine between children aged 3-5 years and infants aged 6-35 months.

Background: The Sinovac enterovirus 71 (EV71) vaccine has shown good safety, immunogenicity, and efficacy in infants aged 6-35 months, whom are considered as the priority of the target population. However, 3-5 years old children accounted for approximately 30% of HFMD cases and are also worth our attention.

Methods: A randomized, double-blind, placebo-controlled, batch-to-batch consistency clinical trial enrolling 1400 participants aged 6-59 months was performed.

Encoding and display with stereo split-ring resonator arrays.

In this Letter, we report on encoding and display based on stereo standing U-shaped resonator (SUSR) arrays. The SUSR serves as a perfect absorber at a structure-dependent frequency when the polarization of incident light is parallel to the bottom rim of the SUSR. When the incidence polarization is rotated for 90° (perpendicular to the bottom rim of the SUSR), the SUSR turns to a perfect reflector at broadband frequencies.

Two-year efficacy and immunogenicity of Sinovac Enterovirus 71 vaccine against hand, foot and mouth disease in children.

Objectives: To evaluate the efficacy and immunogenicity of the Sinovac Enterovirus 71 (EV71) vaccine for up to two years.

Methods: We did a follow-up study of our initial randomized trial in 10,077 participants, who were randomized to receive EV71 vaccine or placebo in a 1:1 ratio and followed for 14 months. The extended follow-up study lasted for another 12 months and EV71-associated hand, foot, and mouth disease (HFMD) was the primary endpoint.

Immunogenicity, safety, and immune persistence of a novel inactivated human enterovirus 71 vaccine: a phase II, Randomized, double-blind, placebo-controlled Trial.

Background: Vaccination is considered a top priority for the control of human enterovirus 71 (EV71) infection outbreaks.

Methods: On the basis of phase I trial results, we conducted a double-blind, randomized, controlled trial to evaluate the optimal dose, immunogenicity, safety and immune persistence of the vaccine. A total of 480 healthy infants were randomly assigned to receive 2 injections of 100 U of vaccine, 200 U of vaccine, 400 U of vaccine, or placebo.

[Development on timely first dose of hepatitis B vaccine for infants].

The study on timely birth dose of hepatitis B vaccine for infants were reviewed in the article. The authors recommend that some relevant researches are necessary to be implemented in the areas which the coverage of HepB timely birth dose is at a low level in order to find out the influencing factors and intervention strategies to protect more children against hepatitis B virus infection as soon as possible.

[Making optimal operation for a BNR process: modeling prediction and experimental verification].

Based on the process model of a BNR system (BCFS), the effects of operational parameters on the effluent quality were predicted by modeling, and were testified simultaneously by a lab-scale experiment, from which almost the same results in the modeling and the experiment were obtained. This means that modeling can be realizably applied to make the optimal operation schemes regardless of pilot-scale and/or full-scale experiments. Both the modeling and the experiment demonstrated that the bio-P removal performance was not influenced by the biomass amount in the anaerobic tank when the returned ratio (rA ) reached 1.

[Cloning and efficient prokaryotic expression of soluble stage-specific antigen cC1 from Cysticercus cellulosae].

Objective: To clone the coding gene of the stage-specific antigen cC1 from Cysticercus cellulosae and express high levels of soluble cC1 in E.coli.

Methods: The cC1 gene was amplified from Cysticercus cellulosae by RT-PCR and cloned into pMD18-T vector, followed by subcloning into the prokaryotic expression plasmid pET28a.

[Effect of hepatitis health promotion project in schools of Beijing and Gansu].

Objective: To evaluate effect of health promotion project of element school students in Beijing and Gansu.

Methods: Evaluation field was conducted in 6 schools of hepatitis health promotion project. They were selected randomly from Beijing and Gansu, questionnaires were given to students and teachers for evaluating the awareness to hepatitis transmission and prevention, telephone interview was given to student's parents for awareness to hepatitis knowledge.

[Experimental study on the effect of COD/P ratios and phosphate recovery on a BNR system].

Based on a BNR system--BCFS, the effect of COD/P and phosphate recovery on the BCFS process was experimentally investigated. The results indicate that a higher P-removal efficiency (87%) and a good P-effluent quality (< or = 0.8 mg x L(-1)) could be maintained at COD/P ratios higher than 24.

[Study on the probability of hepatitis B virus infection at public service places].

Objective: To provide data for the control and prevention of hepatitis B and HBV surface antigen (HBsAg) status among the appliances and practitioners working in the public service places.

Methods: 63 beauty parlors, barber shops and bathing centers selected under stratified randomization sampling method and 682 workers were investigated through questionnaire. HBsAg from th e appliances of the public service places and employee was detected by RIA.

[Transformation of schistosomulae by electroporation and transient expression of the enhanced green fluorescent protein (EGFP) gene].

Objective: To explore the possibility of heterogeneous gene to express in juvenile Schistosoma japonicum and the application of electroporation in transformation of schistosomulae.

Methods: The plasmids of pEGFP-C1 were introduced into mechanically transformed schistosomula with electroporation. The presence, transcription and translation of the transgene in electroporated schistosomula were confirmed by PCR, RT-PCR and Western blotting analysis respectively using the genomic DNA, total RNA and protein extracted and isolated from schistosomula cultured in vitro for 48 hours.